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Next generation genetically encoded fluorescent sensors for serotonin

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Next generation genetically encoded fluorescent sensors for serotonin. / Kubitschke, Martin; Müller, Monika; Wallhorn, Lutz; Pulin, Mauro; Mittag, Manuel; Pollok, Stefan; Ziebarth, Tim; Bremshey, Svenja; Gerdey, Jill; Claussen, Kristin Carolin; Renken, Kim; Groß, Juliana; Gneiße, Pascal; Meyer, Niklas; Wiegert, J Simon; Reiner, Andreas; Fuhrmann, Martin; Masseck, Olivia Andrea.

In: NAT COMMUN, Vol. 13, No. 1, 7525, 06.12.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kubitschke, M, Müller, M, Wallhorn, L, Pulin, M, Mittag, M, Pollok, S, Ziebarth, T, Bremshey, S, Gerdey, J, Claussen, KC, Renken, K, Groß, J, Gneiße, P, Meyer, N, Wiegert, JS, Reiner, A, Fuhrmann, M & Masseck, OA 2022, 'Next generation genetically encoded fluorescent sensors for serotonin', NAT COMMUN, vol. 13, no. 1, 7525. https://doi.org/10.1038/s41467-022-35200-w

APA

Kubitschke, M., Müller, M., Wallhorn, L., Pulin, M., Mittag, M., Pollok, S., Ziebarth, T., Bremshey, S., Gerdey, J., Claussen, K. C., Renken, K., Groß, J., Gneiße, P., Meyer, N., Wiegert, J. S., Reiner, A., Fuhrmann, M., & Masseck, O. A. (2022). Next generation genetically encoded fluorescent sensors for serotonin. NAT COMMUN, 13(1), [7525]. https://doi.org/10.1038/s41467-022-35200-w

Vancouver

Kubitschke M, Müller M, Wallhorn L, Pulin M, Mittag M, Pollok S et al. Next generation genetically encoded fluorescent sensors for serotonin. NAT COMMUN. 2022 Dec 6;13(1). 7525. https://doi.org/10.1038/s41467-022-35200-w

Bibtex

@article{5deca88bd5924c6ebda7ac4ee35503e1,
title = "Next generation genetically encoded fluorescent sensors for serotonin",
abstract = "We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.",
author = "Martin Kubitschke and Monika M{\"u}ller and Lutz Wallhorn and Mauro Pulin and Manuel Mittag and Stefan Pollok and Tim Ziebarth and Svenja Bremshey and Jill Gerdey and Claussen, {Kristin Carolin} and Kim Renken and Juliana Gro{\ss} and Pascal Gnei{\ss}e and Niklas Meyer and Wiegert, {J Simon} and Andreas Reiner and Martin Fuhrmann and Masseck, {Olivia Andrea}",
note = "{\textcopyright} 2022. The Author(s).",
year = "2022",
month = dec,
day = "6",
doi = "10.1038/s41467-022-35200-w",
language = "English",
volume = "13",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Next generation genetically encoded fluorescent sensors for serotonin

AU - Kubitschke, Martin

AU - Müller, Monika

AU - Wallhorn, Lutz

AU - Pulin, Mauro

AU - Mittag, Manuel

AU - Pollok, Stefan

AU - Ziebarth, Tim

AU - Bremshey, Svenja

AU - Gerdey, Jill

AU - Claussen, Kristin Carolin

AU - Renken, Kim

AU - Groß, Juliana

AU - Gneiße, Pascal

AU - Meyer, Niklas

AU - Wiegert, J Simon

AU - Reiner, Andreas

AU - Fuhrmann, Martin

AU - Masseck, Olivia Andrea

N1 - © 2022. The Author(s).

PY - 2022/12/6

Y1 - 2022/12/6

N2 - We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.

AB - We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging.

U2 - 10.1038/s41467-022-35200-w

DO - 10.1038/s41467-022-35200-w

M3 - SCORING: Journal article

C2 - 36473867

VL - 13

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

M1 - 7525

ER -