Research ExplorerPublicationsNew Guanidine Alkaloids Batzelladines O and P from the Marin...

New Guanidine Alkaloids Batzelladines O and P from the Marine Sponge Monanchora pulchra Induce Apoptosis and Autophagy in Prostate Cancer Cells

Standard

New Guanidine Alkaloids Batzelladines O and P from the Marine Sponge Monanchora pulchra Induce Apoptosis and Autophagy in Prostate Cancer Cells. / Dyshlovoy, Sergey A.; Shubina, Larisa K.; Makarieva, Tatyana N.; Guzii, Alla G.; Hauschild, Jessica; Strewinsky, Nadja; Berdyshev, Dmitrii V.; Kudryashova, Ekaterina K.; Menshov, Alexander S.; Popov, Roman S.; Dmitrenok, Pavel S.; Graefen, Markus; Bokemeyer, Carsten; Amsberg, Gunhild von.

In: MAR DRUGS, Vol. 20, No. 12, 738, 25.11.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Dyshlovoy, SA, Shubina, LK, Makarieva, TN, Guzii, AG, Hauschild, J, Strewinsky, N, Berdyshev, DV, Kudryashova, EK, Menshov, AS, Popov, RS, Dmitrenok, PS, Graefen, M, Bokemeyer, C & Amsberg, GV 2022, 'New Guanidine Alkaloids Batzelladines O and P from the Marine Sponge Monanchora pulchra Induce Apoptosis and Autophagy in Prostate Cancer Cells', MAR DRUGS, vol. 20, no. 12, 738. https://doi.org/10.3390/md20120738

APA

Dyshlovoy, S. A., Shubina, L. K., Makarieva, T. N., Guzii, A. G., Hauschild, J., Strewinsky, N., Berdyshev, D. V., Kudryashova, E. K., Menshov, A. S., Popov, R. S., Dmitrenok, P. S., Graefen, M., Bokemeyer, C., & Amsberg, G. V. (2022). New Guanidine Alkaloids Batzelladines O and P from the Marine Sponge Monanchora pulchra Induce Apoptosis and Autophagy in Prostate Cancer Cells. MAR DRUGS, 20(12), [738]. https://doi.org/10.3390/md20120738

Vancouver

Dyshlovoy SA, Shubina LK, Makarieva TN, Guzii AG, Hauschild J, Strewinsky N et al. New Guanidine Alkaloids Batzelladines O and P from the Marine Sponge Monanchora pulchra Induce Apoptosis and Autophagy in Prostate Cancer Cells. MAR DRUGS. 2022 Nov 25;20(12). 738. https://doi.org/10.3390/md20120738

Bibtex

@article{28c5a1c2be914db3b4d1042d586d6bfd,
title = "New Guanidine Alkaloids Batzelladines O and P from the Marine Sponge Monanchora pulchra Induce Apoptosis and Autophagy in Prostate Cancer Cells",
abstract = "Two new guanidine alkaloids, batzelladines O (1) and P (2), were isolated from the deep-water marine sponge Monanchora pulchra. The structures of these metabolites were determined by NMR spectroscopy, mass spectrometry, and ECD. The isolated compounds exhibited cytotoxic activity in human prostate cancer cells PC3, PC3-DR, and 22Rv1 at low micromolar concentrations and inhibited colony formation and survival of the cancer cells. Batzelladines O (1) and P (2) induced apoptosis, which was detected by Western blotting as caspase-3 and PARP cleavage. Additionally, induction of pro-survival autophagy indicated as upregulation of LC3B-II and suppression of mTOR was observed in the treated cells. In line with this, the combination with autophagy inhibitor 3-methyladenine synergistically increased the cytotoxic activity of batzelladines O (1) and P (2). Both compounds were equally active in docetaxel-sensitive and docetaxel-resistant prostate cancer cells, despite exhibiting a slight p-glycoprotein substrate-like activity. In combination with docetaxel, an additive effect was observed. In conclusion, the isolated new guanidine alkaloids are promising drug candidates for the treatment of taxane-resistant prostate cancer.",
keywords = "Animals, Male, Humans, Guanidine/pharmacology, Docetaxel/pharmacology, Guanidines/pharmacology, Porifera/chemistry, Apoptosis, Cell Line, Tumor, Antineoplastic Agents/pharmacology, Prostatic Neoplasms/drug therapy, Autophagy, Alkaloids/pharmacology",
author = "Dyshlovoy, {Sergey A.} and Shubina, {Larisa K.} and Makarieva, {Tatyana N.} and Guzii, {Alla G.} and Jessica Hauschild and Nadja Strewinsky and Berdyshev, {Dmitrii V.} and Kudryashova, {Ekaterina K.} and Menshov, {Alexander S.} and Popov, {Roman S.} and Dmitrenok, {Pavel S.} and Markus Graefen and Carsten Bokemeyer and Amsberg, {Gunhild von}",
year = "2022",
month = nov,
day = "25",
doi = "10.3390/md20120738",
language = "English",
volume = "20",
journal = "MAR DRUGS",
issn = "1660-3397",
publisher = "MDPI AG",
number = "12",

}

RIS

TY - JOUR

T1 - New Guanidine Alkaloids Batzelladines O and P from the Marine Sponge Monanchora pulchra Induce Apoptosis and Autophagy in Prostate Cancer Cells

AU - Dyshlovoy, Sergey A.

AU - Shubina, Larisa K.

AU - Makarieva, Tatyana N.

AU - Guzii, Alla G.

AU - Hauschild, Jessica

AU - Strewinsky, Nadja

AU - Berdyshev, Dmitrii V.

AU - Kudryashova, Ekaterina K.

AU - Menshov, Alexander S.

AU - Popov, Roman S.

AU - Dmitrenok, Pavel S.

AU - Graefen, Markus

AU - Bokemeyer, Carsten

AU - Amsberg, Gunhild von

PY - 2022/11/25

Y1 - 2022/11/25

N2 - Two new guanidine alkaloids, batzelladines O (1) and P (2), were isolated from the deep-water marine sponge Monanchora pulchra. The structures of these metabolites were determined by NMR spectroscopy, mass spectrometry, and ECD. The isolated compounds exhibited cytotoxic activity in human prostate cancer cells PC3, PC3-DR, and 22Rv1 at low micromolar concentrations and inhibited colony formation and survival of the cancer cells. Batzelladines O (1) and P (2) induced apoptosis, which was detected by Western blotting as caspase-3 and PARP cleavage. Additionally, induction of pro-survival autophagy indicated as upregulation of LC3B-II and suppression of mTOR was observed in the treated cells. In line with this, the combination with autophagy inhibitor 3-methyladenine synergistically increased the cytotoxic activity of batzelladines O (1) and P (2). Both compounds were equally active in docetaxel-sensitive and docetaxel-resistant prostate cancer cells, despite exhibiting a slight p-glycoprotein substrate-like activity. In combination with docetaxel, an additive effect was observed. In conclusion, the isolated new guanidine alkaloids are promising drug candidates for the treatment of taxane-resistant prostate cancer.

AB - Two new guanidine alkaloids, batzelladines O (1) and P (2), were isolated from the deep-water marine sponge Monanchora pulchra. The structures of these metabolites were determined by NMR spectroscopy, mass spectrometry, and ECD. The isolated compounds exhibited cytotoxic activity in human prostate cancer cells PC3, PC3-DR, and 22Rv1 at low micromolar concentrations and inhibited colony formation and survival of the cancer cells. Batzelladines O (1) and P (2) induced apoptosis, which was detected by Western blotting as caspase-3 and PARP cleavage. Additionally, induction of pro-survival autophagy indicated as upregulation of LC3B-II and suppression of mTOR was observed in the treated cells. In line with this, the combination with autophagy inhibitor 3-methyladenine synergistically increased the cytotoxic activity of batzelladines O (1) and P (2). Both compounds were equally active in docetaxel-sensitive and docetaxel-resistant prostate cancer cells, despite exhibiting a slight p-glycoprotein substrate-like activity. In combination with docetaxel, an additive effect was observed. In conclusion, the isolated new guanidine alkaloids are promising drug candidates for the treatment of taxane-resistant prostate cancer.

KW - Animals

KW - Male

KW - Humans

KW - Guanidine/pharmacology

KW - Docetaxel/pharmacology

KW - Guanidines/pharmacology

KW - Porifera/chemistry

KW - Apoptosis

KW - Cell Line, Tumor

KW - Antineoplastic Agents/pharmacology

KW - Prostatic Neoplasms/drug therapy

KW - Autophagy

KW - Alkaloids/pharmacology

U2 - 10.3390/md20120738

DO - 10.3390/md20120738

M3 - SCORING: Journal article

C2 - 36547885

VL - 20

JO - MAR DRUGS

JF - MAR DRUGS

SN - 1660-3397

IS - 12

M1 - 738

ER -