Nestin in human Skin: exclusive expression in intramesenchymal skin compartments and regulation by leptin
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Nestin in human Skin: exclusive expression in intramesenchymal skin compartments and regulation by leptin. / Tiede, Stephan; Kloepper, Jennifer E; Ernst, Nancy; Poeggeler, Burkard; Kruse, Charli; Paus, Ralf.
In: J INVEST DERMATOL, Vol. 129, No. 11, 11.2009, p. 2711-20.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Nestin in human Skin: exclusive expression in intramesenchymal skin compartments and regulation by leptin
AU - Tiede, Stephan
AU - Kloepper, Jennifer E
AU - Ernst, Nancy
AU - Poeggeler, Burkard
AU - Kruse, Charli
AU - Paus, Ralf
PY - 2009/11
Y1 - 2009/11
N2 - Cutaneous nestin+ cells are of substantial interest in regenerative medicine. However, the location of nestin+ cells in situ remains controversial. We therefore sought to determine their location in female human scalp skin, using stringently controlled immunohistochemical techniques, Western blot analysis, and in situ hybridization and complementing those techniques with relative and quantitative reverse transcriptase-PCR of enzymatically digested or laser-capture microdissected human hair follicle (HF) compartments. We show here that the immunoreactivity (IR) patterns obtained with anti-nestin antibodies are highly dependent on the tissue-fixation and immunohistochemical methods used. NESTIN mRNA could not be detected within HF-associated epithelial cells in situ or in RNA extracts of the microdissected HF epithelium. Instead, NESTIN transcripts were found only in intramesenchymal skin compartments. Individual cells showing both, specific nestin IR and NESTIN mRNA were detectable in the connective-tissue sheaths of human HFs, sebaceous and sweat glands. Moreover, stimulation of organ-cultured human scalp skin with the adipokine leptin increased the number of nestin+ cells in these intramesenchymal skin locations, whereas no specific nestin IR could be induced by leptin within the HF epithelium, including the bulge. Therefore, nestin expression at the gene and protein levels in human scalp skin is restricted to the periappendage mesenchyme and can be stimulated by leptin.
AB - Cutaneous nestin+ cells are of substantial interest in regenerative medicine. However, the location of nestin+ cells in situ remains controversial. We therefore sought to determine their location in female human scalp skin, using stringently controlled immunohistochemical techniques, Western blot analysis, and in situ hybridization and complementing those techniques with relative and quantitative reverse transcriptase-PCR of enzymatically digested or laser-capture microdissected human hair follicle (HF) compartments. We show here that the immunoreactivity (IR) patterns obtained with anti-nestin antibodies are highly dependent on the tissue-fixation and immunohistochemical methods used. NESTIN mRNA could not be detected within HF-associated epithelial cells in situ or in RNA extracts of the microdissected HF epithelium. Instead, NESTIN transcripts were found only in intramesenchymal skin compartments. Individual cells showing both, specific nestin IR and NESTIN mRNA were detectable in the connective-tissue sheaths of human HFs, sebaceous and sweat glands. Moreover, stimulation of organ-cultured human scalp skin with the adipokine leptin increased the number of nestin+ cells in these intramesenchymal skin locations, whereas no specific nestin IR could be induced by leptin within the HF epithelium, including the bulge. Therefore, nestin expression at the gene and protein levels in human scalp skin is restricted to the periappendage mesenchyme and can be stimulated by leptin.
KW - Antibody Specificity
KW - Blotting, Western
KW - Epithelial Cells
KW - Female
KW - Hair Follicle
KW - Humans
KW - Immunohistochemistry
KW - In Situ Hybridization
KW - Intermediate Filament Proteins
KW - Leptin
KW - Mesenchymal Stromal Cells
KW - Middle Aged
KW - Nerve Tissue Proteins
KW - Nestin
KW - Organ Culture Techniques
KW - Scalp
KW - Sebaceous Glands
KW - Sweat Glands
KW - Tissue Fixation
KW - Transcription, Genetic
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1038/jid.2009.148
DO - 10.1038/jid.2009.148
M3 - SCORING: Journal article
C2 - 19554024
VL - 129
SP - 2711
EP - 2720
JO - J INVEST DERMATOL
JF - J INVEST DERMATOL
SN - 0022-202X
IS - 11
ER -