Nanodomain coupling between Ca2+ channels and Ca2+ sensors promotes fast and efficient transmitter release at a cortical GABAergic synapse.
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Nanodomain coupling between Ca2+ channels and Ca2+ sensors promotes fast and efficient transmitter release at a cortical GABAergic synapse. / Bucurenciu, Iancu; Kulik, Akos; Schwaller, Beat; Frotscher, Michael; Jonas, Peter.
In: NEURON, Vol. 57, No. 4, 4, 2008, p. 536-545.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Nanodomain coupling between Ca2+ channels and Ca2+ sensors promotes fast and efficient transmitter release at a cortical GABAergic synapse.
AU - Bucurenciu, Iancu
AU - Kulik, Akos
AU - Schwaller, Beat
AU - Frotscher, Michael
AU - Jonas, Peter
PY - 2008
Y1 - 2008
N2 - It is generally thought that transmitter release at mammalian central synapses is triggered by Ca2+ microdomains, implying loose coupling between presynaptic Ca2+ channels and Ca2+ sensors of exocytosis. Here we show that Ca2+ channel subunit immunoreactivity is highly concentrated in the active zone of GABAergic presynaptic terminals of putative parvalbumin-containing basket cells in the hippocampus. Paired recording combined with presynaptic patch pipette perfusion revealed that GABA release at basket cell-granule cell synapses is sensitive to millimolar concentrations of the fast Ca2+ chelator BAPTA but insensitive to the slow Ca2+ chelator EGTA. These results show that Ca2+ source and Ca2+ sensor are tightly coupled at this synapse, with distances in the range of 10-20 nm. Models of Ca2+ inflow-exocytosis coupling further reveal that the tightness of coupling increases efficacy, speed, and temporal precision of transmitter release. Thus, tight coupling contributes to fast feedforward and feedback inhibition in the hippocampal network.
AB - It is generally thought that transmitter release at mammalian central synapses is triggered by Ca2+ microdomains, implying loose coupling between presynaptic Ca2+ channels and Ca2+ sensors of exocytosis. Here we show that Ca2+ channel subunit immunoreactivity is highly concentrated in the active zone of GABAergic presynaptic terminals of putative parvalbumin-containing basket cells in the hippocampus. Paired recording combined with presynaptic patch pipette perfusion revealed that GABA release at basket cell-granule cell synapses is sensitive to millimolar concentrations of the fast Ca2+ chelator BAPTA but insensitive to the slow Ca2+ chelator EGTA. These results show that Ca2+ source and Ca2+ sensor are tightly coupled at this synapse, with distances in the range of 10-20 nm. Models of Ca2+ inflow-exocytosis coupling further reveal that the tightness of coupling increases efficacy, speed, and temporal precision of transmitter release. Thus, tight coupling contributes to fast feedforward and feedback inhibition in the hippocampal network.
KW - Animals
KW - Calcium Signaling physiology
KW - Rats
KW - Synaptic Transmission physiology
KW - Calcium Channels metabolism
KW - Hippocampus metabolism
KW - Nanotechnology methods
KW - Rats, Wistar
KW - Synapses metabolism
KW - gamma-Aminobutyric Acid metabolism
KW - Animals
KW - Calcium Signaling physiology
KW - Rats
KW - Synaptic Transmission physiology
KW - Calcium Channels metabolism
KW - Hippocampus metabolism
KW - Nanotechnology methods
KW - Rats, Wistar
KW - Synapses metabolism
KW - gamma-Aminobutyric Acid metabolism
M3 - SCORING: Zeitschriftenaufsatz
VL - 57
SP - 536
EP - 545
JO - NEURON
JF - NEURON
SN - 0896-6273
IS - 4
M1 - 4
ER -
