Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons.

Wolfgang Wagner; Stephan D Brenowitz; John A Hammer

Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons.

Standard

Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons. / Wagner, Wolfgang; Brenowitz, Stephan D; Hammer, John A.

In: NAT CELL BIOL, Vol. 13, No. 1, 1, 2011, p. 40-48.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wagner, W, Brenowitz, SD & Hammer, JA 2011, 'Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons.', NAT CELL BIOL, vol. 13, no. 1, 1, pp. 40-48. <http://www.ncbi.nlm.nih.gov/pubmed/21151132?dopt=Citation>

APA

Wagner, W., Brenowitz, S. D., & Hammer, J. A. (2011). Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons. NAT CELL BIOL, 13(1), 40-48. [1]. http://www.ncbi.nlm.nih.gov/pubmed/21151132?dopt=Citation

Vancouver

Wagner W, Brenowitz SD, Hammer JA. Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons. NAT CELL BIOL. 2011;13(1):40-48. 1.

Bibtex

@article{dab7cdd63b4346a59af6393b13c36c0a,

title = "Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons.",

abstract = "Extension of the endoplasmic reticulum (ER) into dendritic spines of Purkinje neurons is required for cerebellar synaptic plasticity and is disrupted in animals with null mutations in Myo5a, the gene encoding myosin-Va. We show here that myosin-Va acts as a point-to-point organelle transporter to pull ER as cargo into Purkinje neuron spines. Specifically, myosin-Va accumulates at the ER tip as the organelle moves into spines, and hydrolysis of ATP by myosin-Va is required for spine ER targeting. Moreover, myosin-Va is responsible for almost all of the spine ER insertion events. Finally, attenuation of the ability of myosin-Va to move along actin filaments reduces the maximum velocity of ER movement into spines, providing direct evidence that myosin-Va drives ER motility. Thus, we have established that an actin-based motor moves ER within animal cells, and have uncovered the mechanism for ER localization to Purkinje neuron spines, a prerequisite for synaptic plasticity.",

keywords = "Animals, Male, Female, Cells, Cultured, Mice, Mice, Inbred C57BL, Mice, Knockout, Kinetics, Models, Neurological, Microscopy, Confocal, Biological Transport, Calcium/metabolism, Endoplasmic Reticulum/*metabolism, Hydrolysis, Adenosine Triphosphate/metabolism, Microtubules/metabolism, Green Fluorescent Proteins/genetics/metabolism, Actin Cytoskeleton/metabolism, Cerebellum/cytology/metabolism, Dendritic Spines/*metabolism, Guanylate Kinase, Intracellular Signaling Peptides and Proteins/genetics/metabolism, Membrane Proteins/genetics/metabolism, Myosin Heavy Chains/genetics/*metabolism, Myosin Type V/genetics/*metabolism, Purkinje Cells/*metabolism, Receptors, AMPA/metabolism, Animals, Male, Female, Cells, Cultured, Mice, Mice, Inbred C57BL, Mice, Knockout, Kinetics, Models, Neurological, Microscopy, Confocal, Biological Transport, Calcium/metabolism, Endoplasmic Reticulum/*metabolism, Hydrolysis, Adenosine Triphosphate/metabolism, Microtubules/metabolism, Green Fluorescent Proteins/genetics/metabolism, Actin Cytoskeleton/metabolism, Cerebellum/cytology/metabolism, Dendritic Spines/*metabolism, Guanylate Kinase, Intracellular Signaling Peptides and Proteins/genetics/metabolism, Membrane Proteins/genetics/metabolism, Myosin Heavy Chains/genetics/*metabolism, Myosin Type V/genetics/*metabolism, Purkinje Cells/*metabolism, Receptors, AMPA/metabolism",

author = "Wolfgang Wagner and Brenowitz, {Stephan D} and Hammer, {John A}",

year = "2011",

language = "English",

volume = "13",

pages = "40--48",

journal = "NAT CELL BIOL",

issn = "1465-7392",

publisher = "NATURE PUBLISHING GROUP",

number = "1",

}

RIS

TY - JOUR

T1 - Myosin-Va transports the endoplasmic reticulum into the dendritic spines of Purkinje neurons.

AU - Wagner, Wolfgang

AU - Brenowitz, Stephan D

AU - Hammer, John A

PY - 2011

Y1 - 2011

N2 - Extension of the endoplasmic reticulum (ER) into dendritic spines of Purkinje neurons is required for cerebellar synaptic plasticity and is disrupted in animals with null mutations in Myo5a, the gene encoding myosin-Va. We show here that myosin-Va acts as a point-to-point organelle transporter to pull ER as cargo into Purkinje neuron spines. Specifically, myosin-Va accumulates at the ER tip as the organelle moves into spines, and hydrolysis of ATP by myosin-Va is required for spine ER targeting. Moreover, myosin-Va is responsible for almost all of the spine ER insertion events. Finally, attenuation of the ability of myosin-Va to move along actin filaments reduces the maximum velocity of ER movement into spines, providing direct evidence that myosin-Va drives ER motility. Thus, we have established that an actin-based motor moves ER within animal cells, and have uncovered the mechanism for ER localization to Purkinje neuron spines, a prerequisite for synaptic plasticity.

AB - Extension of the endoplasmic reticulum (ER) into dendritic spines of Purkinje neurons is required for cerebellar synaptic plasticity and is disrupted in animals with null mutations in Myo5a, the gene encoding myosin-Va. We show here that myosin-Va acts as a point-to-point organelle transporter to pull ER as cargo into Purkinje neuron spines. Specifically, myosin-Va accumulates at the ER tip as the organelle moves into spines, and hydrolysis of ATP by myosin-Va is required for spine ER targeting. Moreover, myosin-Va is responsible for almost all of the spine ER insertion events. Finally, attenuation of the ability of myosin-Va to move along actin filaments reduces the maximum velocity of ER movement into spines, providing direct evidence that myosin-Va drives ER motility. Thus, we have established that an actin-based motor moves ER within animal cells, and have uncovered the mechanism for ER localization to Purkinje neuron spines, a prerequisite for synaptic plasticity.

KW - Animals

KW - Male

KW - Female

KW - Cells, Cultured

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Kinetics

KW - Models, Neurological

KW - Microscopy, Confocal

KW - Biological Transport

KW - Calcium/metabolism

KW - Endoplasmic Reticulum/metabolism

KW - Hydrolysis

KW - Adenosine Triphosphate/metabolism

KW - Microtubules/metabolism

KW - Green Fluorescent Proteins/genetics/metabolism

KW - Actin Cytoskeleton/metabolism

KW - Cerebellum/cytology/metabolism

KW - Dendritic Spines/metabolism

KW - Guanylate Kinase

KW - Intracellular Signaling Peptides and Proteins/genetics/metabolism

KW - Membrane Proteins/genetics/metabolism

KW - Myosin Heavy Chains/genetics/metabolism

KW - Myosin Type V/genetics/metabolism

KW - Purkinje Cells/metabolism

KW - Receptors, AMPA/metabolism