Myelin basic protein and neurofilament H in postmortem cerebrospinal fluid as surrogate markers of fatal traumatic brain injury
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Myelin basic protein and neurofilament H in postmortem cerebrospinal fluid as surrogate markers of fatal traumatic brain injury. / Bohnert, Simone; Wirth, Christoph; Schmitz, Werner; Trella, Stefanie; Monoranu, Camelia-Maria; Ondruschka, Benjamin; Bohnert, Michael.
In: INT J LEGAL MED, Vol. 135, No. 4, 07.2021, p. 1525-1535.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Myelin basic protein and neurofilament H in postmortem cerebrospinal fluid as surrogate markers of fatal traumatic brain injury
AU - Bohnert, Simone
AU - Wirth, Christoph
AU - Schmitz, Werner
AU - Trella, Stefanie
AU - Monoranu, Camelia-Maria
AU - Ondruschka, Benjamin
AU - Bohnert, Michael
PY - 2021/7
Y1 - 2021/7
N2 - The aim of this study was to investigate if the biomarkers myelin basic protein (MBP) and neurofilament-H (NF-H) yielded informative value in forensic diagnostics when examining cadaveric cerebrospinal fluid (CSF) biochemically via an enzyme-linked immunosorbent assay (ELISA) and comparing the corresponding brain tissue in fatal traumatic brain injury (TBI) autopsy cases by immunocytochemistry versus immunohistochemistry. In 21 trauma and 19 control cases, CSF was collected semi-sterile after suboccipital puncture and brain specimens after preparation. The CSF MBP (p = 0.006) and NF-H (p = 0.0002) levels after TBI were significantly higher than those in cardiovascular controls. Immunohistochemical staining against MBP and against NF-H was performed on cortical and subcortical samples from also biochemically investigated cases (5 TBI cases/5 controls). Compared to the controls, the TBI cases showed a visually reduced staining reaction against MBP or repeatedly ruptured neurofilaments against NF-H. Immunocytochemical tests showed MBP-positive phagocytizing macrophages in CSF with a survival time of > 24 h. In addition, numerous TMEM119-positive microglia could be detected with different degrees of staining intensity in the CSF of trauma cases. As a result, we were able to document that elevated levels of MBP and NF-H in the CSF should be considered as useful neuroinjury biomarkers of traumatic brain injury.
AB - The aim of this study was to investigate if the biomarkers myelin basic protein (MBP) and neurofilament-H (NF-H) yielded informative value in forensic diagnostics when examining cadaveric cerebrospinal fluid (CSF) biochemically via an enzyme-linked immunosorbent assay (ELISA) and comparing the corresponding brain tissue in fatal traumatic brain injury (TBI) autopsy cases by immunocytochemistry versus immunohistochemistry. In 21 trauma and 19 control cases, CSF was collected semi-sterile after suboccipital puncture and brain specimens after preparation. The CSF MBP (p = 0.006) and NF-H (p = 0.0002) levels after TBI were significantly higher than those in cardiovascular controls. Immunohistochemical staining against MBP and against NF-H was performed on cortical and subcortical samples from also biochemically investigated cases (5 TBI cases/5 controls). Compared to the controls, the TBI cases showed a visually reduced staining reaction against MBP or repeatedly ruptured neurofilaments against NF-H. Immunocytochemical tests showed MBP-positive phagocytizing macrophages in CSF with a survival time of > 24 h. In addition, numerous TMEM119-positive microglia could be detected with different degrees of staining intensity in the CSF of trauma cases. As a result, we were able to document that elevated levels of MBP and NF-H in the CSF should be considered as useful neuroinjury biomarkers of traumatic brain injury.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Autopsy
KW - Biomarkers/cerebrospinal fluid
KW - Brain Injuries, Traumatic/diagnosis
KW - Case-Control Studies
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Humans
KW - Immunohistochemistry/methods
KW - Male
KW - Middle Aged
KW - Myelin Basic Protein/cerebrospinal fluid
KW - Neurofilament Proteins/cerebrospinal fluid
U2 - 10.1007/s00414-021-02606-y
DO - 10.1007/s00414-021-02606-y
M3 - SCORING: Journal article
C2 - 33895854
VL - 135
SP - 1525
EP - 1535
JO - INT J LEGAL MED
JF - INT J LEGAL MED
SN - 0937-9827
IS - 4
ER -