Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas
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Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas. / Salg, Gabriel Alexander; Steinle, Verena; Labode, Jonas; Wagner, Willi; Studier-Fischer, Alexander; Reiser, Johanna; Farjallah, Elyes; Guettlein, Michelle; Albers, Jonas; Hilgenfeld, Tim; Giese, Nathalia A; Stiller, Wolfram; Nickel, Felix; Loos, Martin; Michalski, Christoph W; Kauczor, Hans-Ulrich; Hackert, Thilo; Dullin, Christian; Mayer, Philipp; Kenngott, Hannes Goetz.
In: SCI REP-UK, Vol. 14, No. 1, 02.05.2024, p. 10136.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas
AU - Salg, Gabriel Alexander
AU - Steinle, Verena
AU - Labode, Jonas
AU - Wagner, Willi
AU - Studier-Fischer, Alexander
AU - Reiser, Johanna
AU - Farjallah, Elyes
AU - Guettlein, Michelle
AU - Albers, Jonas
AU - Hilgenfeld, Tim
AU - Giese, Nathalia A
AU - Stiller, Wolfram
AU - Nickel, Felix
AU - Loos, Martin
AU - Michalski, Christoph W
AU - Kauczor, Hans-Ulrich
AU - Hackert, Thilo
AU - Dullin, Christian
AU - Mayer, Philipp
AU - Kenngott, Hannes Goetz
N1 - © 2024. The Author(s).
PY - 2024/5/2
Y1 - 2024/5/2
N2 - Exocrine and endocrine pancreas are interconnected anatomically and functionally, with vasculature facilitating bidirectional communication. Our understanding of this network remains limited, largely due to two-dimensional histology and missing combination with three-dimensional imaging. In this study, a multiscale 3D-imaging process was used to analyze a porcine pancreas. Clinical computed tomography, digital volume tomography, micro-computed tomography and Synchrotron-based propagation-based imaging were applied consecutively. Fields of view correlated inversely with attainable resolution from a whole organism level down to capillary structures with a voxel edge length of 2.0 µm. Segmented vascular networks from 3D-imaging data were correlated with tissue sections stained by immunohistochemistry and revealed highly vascularized regions to be intra-islet capillaries of islets of Langerhans. Generated 3D-datasets allowed for three-dimensional qualitative and quantitative organ and vessel structure analysis. Beyond this study, the method shows potential for application across a wide range of patho-morphology analyses and might possibly provide microstructural blueprints for biotissue engineering.
AB - Exocrine and endocrine pancreas are interconnected anatomically and functionally, with vasculature facilitating bidirectional communication. Our understanding of this network remains limited, largely due to two-dimensional histology and missing combination with three-dimensional imaging. In this study, a multiscale 3D-imaging process was used to analyze a porcine pancreas. Clinical computed tomography, digital volume tomography, micro-computed tomography and Synchrotron-based propagation-based imaging were applied consecutively. Fields of view correlated inversely with attainable resolution from a whole organism level down to capillary structures with a voxel edge length of 2.0 µm. Segmented vascular networks from 3D-imaging data were correlated with tissue sections stained by immunohistochemistry and revealed highly vascularized regions to be intra-islet capillaries of islets of Langerhans. Generated 3D-datasets allowed for three-dimensional qualitative and quantitative organ and vessel structure analysis. Beyond this study, the method shows potential for application across a wide range of patho-morphology analyses and might possibly provide microstructural blueprints for biotissue engineering.
KW - Animals
KW - Imaging, Three-Dimensional/methods
KW - Pancreas/diagnostic imaging
KW - Swine
KW - Multimodal Imaging/methods
KW - X-Ray Microtomography/methods
KW - Islets of Langerhans/diagnostic imaging
KW - Tomography, X-Ray Computed/methods
U2 - 10.1038/s41598-024-60254-9
DO - 10.1038/s41598-024-60254-9
M3 - SCORING: Journal article
C2 - 38698049
VL - 14
SP - 10136
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
IS - 1
ER -