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Multiplexing clonality: combining RGB marking and genetic barcoding

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Multiplexing clonality: combining RGB marking and genetic barcoding. / Cornils, Kerstin; Thielecke, Lars; Hüser, Svenja; Forgber, Michael; Thomaschewski, Michael; Kleist, Nadja; Hussein, Kais; Riecken, Kristoffer; Volz, Tassilo; Gerdes, Sebastian; Glauche, Ingmar; Dahl, Andreas; Dandri, Maura; Roeder, Ingo; Fehse, Boris.

In: NUCLEIC ACIDS RES, Vol. 42, No. 7, 01.04.2014, p. e56.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Cornils, K, Thielecke, L, Hüser, S, Forgber, M, Thomaschewski, M, Kleist, N, Hussein, K, Riecken, K, Volz, T, Gerdes, S, Glauche, I, Dahl, A, Dandri, M, Roeder, I & Fehse, B 2014, 'Multiplexing clonality: combining RGB marking and genetic barcoding', NUCLEIC ACIDS RES, vol. 42, no. 7, pp. e56. https://doi.org/10.1093/nar/gku081

APA

Cornils, K., Thielecke, L., Hüser, S., Forgber, M., Thomaschewski, M., Kleist, N., Hussein, K., Riecken, K., Volz, T., Gerdes, S., Glauche, I., Dahl, A., Dandri, M., Roeder, I., & Fehse, B. (2014). Multiplexing clonality: combining RGB marking and genetic barcoding. NUCLEIC ACIDS RES, 42(7), e56. https://doi.org/10.1093/nar/gku081

Vancouver

Cornils K, Thielecke L, Hüser S, Forgber M, Thomaschewski M, Kleist N et al. Multiplexing clonality: combining RGB marking and genetic barcoding. NUCLEIC ACIDS RES. 2014 Apr 1;42(7):e56. https://doi.org/10.1093/nar/gku081

Bibtex

@article{77453088760e4f048731074e1d54adb3,
title = "Multiplexing clonality: combining RGB marking and genetic barcoding",
abstract = "RGB marking and DNA barcoding are two cutting-edge technologies in the field of clonal cell marking. To combine the virtues of both approaches, we equipped LeGO vectors encoding red, green or blue fluorescent proteins with complex DNA barcodes carrying color-specific signatures. For these vectors, we generated highly complex plasmid libraries that were used for the production of barcoded lentiviral vector particles. In proof-of-principle experiments, we used barcoded vectors for RGB marking of cell lines and primary murine hepatocytes. We applied single-cell polymerase chain reaction to decipher barcode signatures of individual RGB-marked cells expressing defined color hues. This enabled us to prove clonal identity of cells with one and the same RGB color. Also, we made use of barcoded vectors to investigate clonal development of leukemia induced by ectopic oncogene expression in murine hematopoietic cells. In conclusion, by combining RGB marking and DNA barcoding, we have established a novel technique for the unambiguous genetic marking of individual cells in the context of normal regeneration as well as malignant outgrowth. Moreover, the introduction of color-specific signatures in barcodes will facilitate studies on the impact of different variables (e.g. vector type, transgenes, culture conditions) in the context of competitive repopulation studies.",
author = "Kerstin Cornils and Lars Thielecke and Svenja H{\"u}ser and Michael Forgber and Michael Thomaschewski and Nadja Kleist and Kais Hussein and Kristoffer Riecken and Tassilo Volz and Sebastian Gerdes and Ingmar Glauche and Andreas Dahl and Maura Dandri and Ingo Roeder and Boris Fehse",
year = "2014",
month = apr,
day = "1",
doi = "10.1093/nar/gku081",
language = "English",
volume = "42",
pages = "e56",
journal = "NUCLEIC ACIDS RES",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "7",

}

RIS

TY - JOUR

T1 - Multiplexing clonality: combining RGB marking and genetic barcoding

AU - Cornils, Kerstin

AU - Thielecke, Lars

AU - Hüser, Svenja

AU - Forgber, Michael

AU - Thomaschewski, Michael

AU - Kleist, Nadja

AU - Hussein, Kais

AU - Riecken, Kristoffer

AU - Volz, Tassilo

AU - Gerdes, Sebastian

AU - Glauche, Ingmar

AU - Dahl, Andreas

AU - Dandri, Maura

AU - Roeder, Ingo

AU - Fehse, Boris

PY - 2014/4/1

Y1 - 2014/4/1

N2 - RGB marking and DNA barcoding are two cutting-edge technologies in the field of clonal cell marking. To combine the virtues of both approaches, we equipped LeGO vectors encoding red, green or blue fluorescent proteins with complex DNA barcodes carrying color-specific signatures. For these vectors, we generated highly complex plasmid libraries that were used for the production of barcoded lentiviral vector particles. In proof-of-principle experiments, we used barcoded vectors for RGB marking of cell lines and primary murine hepatocytes. We applied single-cell polymerase chain reaction to decipher barcode signatures of individual RGB-marked cells expressing defined color hues. This enabled us to prove clonal identity of cells with one and the same RGB color. Also, we made use of barcoded vectors to investigate clonal development of leukemia induced by ectopic oncogene expression in murine hematopoietic cells. In conclusion, by combining RGB marking and DNA barcoding, we have established a novel technique for the unambiguous genetic marking of individual cells in the context of normal regeneration as well as malignant outgrowth. Moreover, the introduction of color-specific signatures in barcodes will facilitate studies on the impact of different variables (e.g. vector type, transgenes, culture conditions) in the context of competitive repopulation studies.

AB - RGB marking and DNA barcoding are two cutting-edge technologies in the field of clonal cell marking. To combine the virtues of both approaches, we equipped LeGO vectors encoding red, green or blue fluorescent proteins with complex DNA barcodes carrying color-specific signatures. For these vectors, we generated highly complex plasmid libraries that were used for the production of barcoded lentiviral vector particles. In proof-of-principle experiments, we used barcoded vectors for RGB marking of cell lines and primary murine hepatocytes. We applied single-cell polymerase chain reaction to decipher barcode signatures of individual RGB-marked cells expressing defined color hues. This enabled us to prove clonal identity of cells with one and the same RGB color. Also, we made use of barcoded vectors to investigate clonal development of leukemia induced by ectopic oncogene expression in murine hematopoietic cells. In conclusion, by combining RGB marking and DNA barcoding, we have established a novel technique for the unambiguous genetic marking of individual cells in the context of normal regeneration as well as malignant outgrowth. Moreover, the introduction of color-specific signatures in barcodes will facilitate studies on the impact of different variables (e.g. vector type, transgenes, culture conditions) in the context of competitive repopulation studies.

U2 - 10.1093/nar/gku081

DO - 10.1093/nar/gku081

M3 - SCORING: Journal article

C2 - 24476916

VL - 42

SP - e56

JO - NUCLEIC ACIDS RES

JF - NUCLEIC ACIDS RES

SN - 0305-1048

IS - 7

ER -