Mouse models of spontaneous liver and lung metastasis for colorectal cancer
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Mouse models of spontaneous liver and lung metastasis for colorectal cancer. / Lücke, Jöran; Mercanoglu, Baris; Zhang, Tao; Zazara, Dimitra E; Zigmond, Ehud; Seeger, Philipp; Mann, Oliver; Izbicki, Jakob R; Hackert, Thilo; Huber, Samuel; Giannou, Anastasios D.
In: STAR PROTOC, Vol. 5, No. 1, 15.03.2024, p. 102811.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Mouse models of spontaneous liver and lung metastasis for colorectal cancer
AU - Lücke, Jöran
AU - Mercanoglu, Baris
AU - Zhang, Tao
AU - Zazara, Dimitra E
AU - Zigmond, Ehud
AU - Seeger, Philipp
AU - Mann, Oliver
AU - Izbicki, Jakob R
AU - Hackert, Thilo
AU - Huber, Samuel
AU - Giannou, Anastasios D
N1 - Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2024/3/15
Y1 - 2024/3/15
N2 - To investigate underlying mechanisms for cancer metastasis and promising therapies in animal models, spontaneous metastasis models can be used to recreate metastasis development. Here, we present three mouse models of spontaneous lung and/or liver metastasis induction. We describe steps for cancer cell preparation, mouse analgesia, and three injection techniques (subcutaneous, intracecal, and intramucosal). We then detail procedures for evaluating metastasis. Most of these models generate metastasis in a time span of 4 weeks in the majority of injected mice. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.
AB - To investigate underlying mechanisms for cancer metastasis and promising therapies in animal models, spontaneous metastasis models can be used to recreate metastasis development. Here, we present three mouse models of spontaneous lung and/or liver metastasis induction. We describe steps for cancer cell preparation, mouse analgesia, and three injection techniques (subcutaneous, intracecal, and intramucosal). We then detail procedures for evaluating metastasis. Most of these models generate metastasis in a time span of 4 weeks in the majority of injected mice. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.
U2 - 10.1016/j.xpro.2023.102811
DO - 10.1016/j.xpro.2023.102811
M3 - SCORING: Journal article
C2 - 38236770
VL - 5
SP - 102811
JO - STAR PROTOC
JF - STAR PROTOC
SN - 2666-1667
IS - 1
ER -