Mouse models of spontaneous liver and lung metastasis for colorectal cancer

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Mouse models of spontaneous liver and lung metastasis for colorectal cancer. / Lücke, Jöran; Mercanoglu, Baris; Zhang, Tao; Zazara, Dimitra E; Zigmond, Ehud; Seeger, Philipp; Mann, Oliver; Izbicki, Jakob R; Hackert, Thilo; Huber, Samuel; Giannou, Anastasios D.

In: STAR PROTOC, Vol. 5, No. 1, 15.03.2024, p. 102811.

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@article{b512820814384fb2849bcb355d23f1e3,
title = "Mouse models of spontaneous liver and lung metastasis for colorectal cancer",
abstract = "To investigate underlying mechanisms for cancer metastasis and promising therapies in animal models, spontaneous metastasis models can be used to recreate metastasis development. Here, we present three mouse models of spontaneous lung and/or liver metastasis induction. We describe steps for cancer cell preparation, mouse analgesia, and three injection techniques (subcutaneous, intracecal, and intramucosal). We then detail procedures for evaluating metastasis. Most of these models generate metastasis in a time span of 4 weeks in the majority of injected mice. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.",
author = "J{\"o}ran L{\"u}cke and Baris Mercanoglu and Tao Zhang and Zazara, {Dimitra E} and Ehud Zigmond and Philipp Seeger and Oliver Mann and Izbicki, {Jakob R} and Thilo Hackert and Samuel Huber and Giannou, {Anastasios D}",
note = "Copyright {\textcopyright} 2023 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2024",
month = mar,
day = "15",
doi = "10.1016/j.xpro.2023.102811",
language = "English",
volume = "5",
pages = "102811",
journal = "STAR PROTOC",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - Mouse models of spontaneous liver and lung metastasis for colorectal cancer

AU - Lücke, Jöran

AU - Mercanoglu, Baris

AU - Zhang, Tao

AU - Zazara, Dimitra E

AU - Zigmond, Ehud

AU - Seeger, Philipp

AU - Mann, Oliver

AU - Izbicki, Jakob R

AU - Hackert, Thilo

AU - Huber, Samuel

AU - Giannou, Anastasios D

N1 - Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2024/3/15

Y1 - 2024/3/15

N2 - To investigate underlying mechanisms for cancer metastasis and promising therapies in animal models, spontaneous metastasis models can be used to recreate metastasis development. Here, we present three mouse models of spontaneous lung and/or liver metastasis induction. We describe steps for cancer cell preparation, mouse analgesia, and three injection techniques (subcutaneous, intracecal, and intramucosal). We then detail procedures for evaluating metastasis. Most of these models generate metastasis in a time span of 4 weeks in the majority of injected mice. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.

AB - To investigate underlying mechanisms for cancer metastasis and promising therapies in animal models, spontaneous metastasis models can be used to recreate metastasis development. Here, we present three mouse models of spontaneous lung and/or liver metastasis induction. We describe steps for cancer cell preparation, mouse analgesia, and three injection techniques (subcutaneous, intracecal, and intramucosal). We then detail procedures for evaluating metastasis. Most of these models generate metastasis in a time span of 4 weeks in the majority of injected mice. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.

U2 - 10.1016/j.xpro.2023.102811

DO - 10.1016/j.xpro.2023.102811

M3 - SCORING: Journal article

C2 - 38236770

VL - 5

SP - 102811

JO - STAR PROTOC

JF - STAR PROTOC

SN - 2666-1667

IS - 1

ER -