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Morphological and functional analysis of rat hepatocyte spheroids generated on poly(L-lactic acid) polymer in a pulsatile flow bioreactor.

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Morphological and functional analysis of rat hepatocyte spheroids generated on poly(L-lactic acid) polymer in a pulsatile flow bioreactor. / Török, Eva; Vogel, Christian; Lütgehetmann, Marc; Ma, Peter X; Dandri-Petersen, Maura; Petersen, Joerg; Burda, Martin R; Siebert, Klaus; Düllmann, Jochen; Rogiers, Xavier; Pollok, Jörg-Matthias.

In: TISSUE ENG, Vol. 12, No. 7, 7, 2006, p. 1881-1890.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Török, E, Vogel, C, Lütgehetmann, M, Ma, PX, Dandri-Petersen, M, Petersen, J, Burda, MR, Siebert, K, Düllmann, J, Rogiers, X & Pollok, J-M 2006, 'Morphological and functional analysis of rat hepatocyte spheroids generated on poly(L-lactic acid) polymer in a pulsatile flow bioreactor.', TISSUE ENG, vol. 12, no. 7, 7, pp. 1881-1890. <http://www.ncbi.nlm.nih.gov/pubmed/16889518?dopt=Citation>

APA

Török, E., Vogel, C., Lütgehetmann, M., Ma, P. X., Dandri-Petersen, M., Petersen, J., Burda, M. R., Siebert, K., Düllmann, J., Rogiers, X., & Pollok, J-M. (2006). Morphological and functional analysis of rat hepatocyte spheroids generated on poly(L-lactic acid) polymer in a pulsatile flow bioreactor. TISSUE ENG, 12(7), 1881-1890. [7]. http://www.ncbi.nlm.nih.gov/pubmed/16889518?dopt=Citation

Vancouver

Török E, Vogel C, Lütgehetmann M, Ma PX, Dandri-Petersen M, Petersen J et al. Morphological and functional analysis of rat hepatocyte spheroids generated on poly(L-lactic acid) polymer in a pulsatile flow bioreactor. TISSUE ENG. 2006;12(7):1881-1890. 7.

Bibtex

@article{9fcb0831b8b1457ab7513615aab4de4f,
title = "Morphological and functional analysis of rat hepatocyte spheroids generated on poly(L-lactic acid) polymer in a pulsatile flow bioreactor.",
abstract = "Liver neo-tissue suitable for transplantation has not been established. Primary rat hepatocytes were cultured on three-dimensional biodegradable polymer matrices in a pulsatile flow bioreactor with the intention of inducing tissue formation and improving cell survival. Functional and structural analysis of the hepatocytes forming liver neo-tissue was performed. Biodegradable poly(L-lactic acid) (PLLA) polymer discs were seeded with 4 x 10(6) primary rat hepatocytes each, were exposed to a pulsatile medium flow of 24 mL/min for 1, 2, 4, or 6 days and were investigated for monoethylglycinexylidine (MEGX) formation, ammonia detoxification, Cytokeratin 18 (CK18) expression, and preserved glycogen storage. Fine structural details were obtained using scanning and transmission electron microscopy. Spheroids of viable hepatocytes were formed. MEGX-specific production was maintained and ammonia removal capacity remained high during the entire flow-culture period of 6 days. CK18 distribution was normal. Periodic-acid- Schiff reaction demonstrated homogenous glycogen storage. The hepatocytes reassembled to form intercellular junctions and bile canaliculi. Functional and morphological analysis of rat hepatocytes forming spheroids in a pulsatile flow bioreactor indicated preserved and intact hepatocyte morphology and specific function. Pulsatile flow culture on PLLA scaffolds is a promising new method of hepatic tissue engineering leading to liver neo-tissue formation.",
author = "Eva T{\"o}r{\"o}k and Christian Vogel and Marc L{\"u}tgehetmann and Ma, {Peter X} and Maura Dandri-Petersen and Joerg Petersen and Burda, {Martin R} and Klaus Siebert and Jochen D{\"u}llmann and Xavier Rogiers and J{\"o}rg-Matthias Pollok",
year = "2006",
language = "Deutsch",
volume = "12",
pages = "1881--1890",
journal = "TISSUE ENG",
issn = "1076-3279",
publisher = "Mary Ann Liebert Inc.",
number = "7",

}

RIS

TY - JOUR

T1 - Morphological and functional analysis of rat hepatocyte spheroids generated on poly(L-lactic acid) polymer in a pulsatile flow bioreactor.

AU - Török, Eva

AU - Vogel, Christian

AU - Lütgehetmann, Marc

AU - Ma, Peter X

AU - Dandri-Petersen, Maura

AU - Petersen, Joerg

AU - Burda, Martin R

AU - Siebert, Klaus

AU - Düllmann, Jochen

AU - Rogiers, Xavier

AU - Pollok, Jörg-Matthias

PY - 2006

Y1 - 2006

N2 - Liver neo-tissue suitable for transplantation has not been established. Primary rat hepatocytes were cultured on three-dimensional biodegradable polymer matrices in a pulsatile flow bioreactor with the intention of inducing tissue formation and improving cell survival. Functional and structural analysis of the hepatocytes forming liver neo-tissue was performed. Biodegradable poly(L-lactic acid) (PLLA) polymer discs were seeded with 4 x 10(6) primary rat hepatocytes each, were exposed to a pulsatile medium flow of 24 mL/min for 1, 2, 4, or 6 days and were investigated for monoethylglycinexylidine (MEGX) formation, ammonia detoxification, Cytokeratin 18 (CK18) expression, and preserved glycogen storage. Fine structural details were obtained using scanning and transmission electron microscopy. Spheroids of viable hepatocytes were formed. MEGX-specific production was maintained and ammonia removal capacity remained high during the entire flow-culture period of 6 days. CK18 distribution was normal. Periodic-acid- Schiff reaction demonstrated homogenous glycogen storage. The hepatocytes reassembled to form intercellular junctions and bile canaliculi. Functional and morphological analysis of rat hepatocytes forming spheroids in a pulsatile flow bioreactor indicated preserved and intact hepatocyte morphology and specific function. Pulsatile flow culture on PLLA scaffolds is a promising new method of hepatic tissue engineering leading to liver neo-tissue formation.

AB - Liver neo-tissue suitable for transplantation has not been established. Primary rat hepatocytes were cultured on three-dimensional biodegradable polymer matrices in a pulsatile flow bioreactor with the intention of inducing tissue formation and improving cell survival. Functional and structural analysis of the hepatocytes forming liver neo-tissue was performed. Biodegradable poly(L-lactic acid) (PLLA) polymer discs were seeded with 4 x 10(6) primary rat hepatocytes each, were exposed to a pulsatile medium flow of 24 mL/min for 1, 2, 4, or 6 days and were investigated for monoethylglycinexylidine (MEGX) formation, ammonia detoxification, Cytokeratin 18 (CK18) expression, and preserved glycogen storage. Fine structural details were obtained using scanning and transmission electron microscopy. Spheroids of viable hepatocytes were formed. MEGX-specific production was maintained and ammonia removal capacity remained high during the entire flow-culture period of 6 days. CK18 distribution was normal. Periodic-acid- Schiff reaction demonstrated homogenous glycogen storage. The hepatocytes reassembled to form intercellular junctions and bile canaliculi. Functional and morphological analysis of rat hepatocytes forming spheroids in a pulsatile flow bioreactor indicated preserved and intact hepatocyte morphology and specific function. Pulsatile flow culture on PLLA scaffolds is a promising new method of hepatic tissue engineering leading to liver neo-tissue formation.

M3 - SCORING: Zeitschriftenaufsatz

VL - 12

SP - 1881

EP - 1890

JO - TISSUE ENG

JF - TISSUE ENG

SN - 1076-3279

IS - 7

M1 - 7

ER -