Monoclonal antibodies for characterization of the heterogeneity of normal and malignant transitional cells.
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Monoclonal antibodies for characterization of the heterogeneity of normal and malignant transitional cells. / Arndt, R; Dürkopf, H; Huland, H; Donn, F; Löning, Thomas; Kalthoff, H.
In: J UROLOGY, Vol. 137, No. 4, 4, 1987, p. 758-763.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Monoclonal antibodies for characterization of the heterogeneity of normal and malignant transitional cells.
AU - Arndt, R
AU - Dürkopf, H
AU - Huland, H
AU - Donn, F
AU - Löning, Thomas
AU - Kalthoff, H
PY - 1987
Y1 - 1987
N2 - We present two monoclonal antibodies (mab), Mano 4/4 and 486 P3/12, directed against tumor-associated antigens of different subpopulations of transitional cell bladder carcinoma tumors. A detailed description is given concerning their reactivity patterns in an ELISA assay against different cell lines. Using immunohistochemical staining against normal bladder mucosa, mab Mano 4/4 reacts with distinct cells in the basal layer and mab 486 P 3/12 recognizes single cells in the superficial layer. Analysing different transitional-cell carcinomas, mab Mano 4/4 reacts with 17 of 20 and mab 486 P 3/12 with 17 of 19 bladder tumors. It is emphasized that both mabs may add new information in respect to a better characterization of the heterogeneity of bladder carcinoma. A biochemical characterization of the mabs and their corresponding antigens is given. Mab Mano 4/4 is directed against a 28 kD glycoprotein and mab 486 P 3/12 is reacting with a 200 kD glycoprotein belonging to the family of CEA-like proteins.
AB - We present two monoclonal antibodies (mab), Mano 4/4 and 486 P3/12, directed against tumor-associated antigens of different subpopulations of transitional cell bladder carcinoma tumors. A detailed description is given concerning their reactivity patterns in an ELISA assay against different cell lines. Using immunohistochemical staining against normal bladder mucosa, mab Mano 4/4 reacts with distinct cells in the basal layer and mab 486 P 3/12 recognizes single cells in the superficial layer. Analysing different transitional-cell carcinomas, mab Mano 4/4 reacts with 17 of 20 and mab 486 P 3/12 with 17 of 19 bladder tumors. It is emphasized that both mabs may add new information in respect to a better characterization of the heterogeneity of bladder carcinoma. A biochemical characterization of the mabs and their corresponding antigens is given. Mab Mano 4/4 is directed against a 28 kD glycoprotein and mab 486 P 3/12 is reacting with a 200 kD glycoprotein belonging to the family of CEA-like proteins.
M3 - SCORING: Zeitschriftenaufsatz
VL - 137
SP - 758
EP - 763
JO - J UROLOGY
JF - J UROLOGY
SN - 0022-5347
IS - 4
M1 - 4
ER -
