Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)

T Matsuyama; A Grossman; H W Mittrücker; D P Siderovski; F Kiefer; T Kawakami; C D Richardson; T Taniguchi; S K Yoshinaga; T W Mak

Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)

Standard

Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE). / Matsuyama, T; Grossman, A; Mittrücker, H W; Siderovski, D P; Kiefer, F; Kawakami, T; Richardson, C D; Taniguchi, T; Yoshinaga, S K; Mak, T W.

In: NUCLEIC ACIDS RES, Vol. 23, No. 12, 25.06.1995, p. 2127-36.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Matsuyama, T, Grossman, A, Mittrücker, HW, Siderovski, DP, Kiefer, F, Kawakami, T, Richardson, CD, Taniguchi, T, Yoshinaga, SK & Mak, TW 1995, 'Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)', NUCLEIC ACIDS RES, vol. 23, no. 12, pp. 2127-36.

APA

Matsuyama, T., Grossman, A., Mittrücker, H. W., Siderovski, D. P., Kiefer, F., Kawakami, T., Richardson, C. D., Taniguchi, T., Yoshinaga, S. K., & Mak, T. W. (1995). Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE). NUCLEIC ACIDS RES, 23(12), 2127-36.

Vancouver

Matsuyama T, Grossman A, Mittrücker HW, Siderovski DP, Kiefer F, Kawakami T et al. Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE). NUCLEIC ACIDS RES. 1995 Jun 25;23(12):2127-36.

Bibtex

@article{4fdaa99cb18f48f79db4298f35aa941e,

title = "Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)",

abstract = "Interferon regulatory factor (IRF) genes encode a family of DNA-binding proteins that are involved in the transcriptional regulation of type-I interferon and/or interferon-inducible genes. We report here the characterization of LSIRF, a new member of the IRF gene family cloned from mouse spleen by the polymerase chain reaction using degenerate primers. LSIRF was found to encode a 51 kDa protein that shares a high degree of amino acid sequence homology in the DNA-binding domain with other IRF family members. LSIRF expression was detectable only in lymphoid cells. In contrast to other IRF genes, LSIRF expression was not induced by interferons, but rather by antigen-receptor mediated stimuli such as plant lectins, CD3 or IgM crosslinking. In in vitro DNA binding studies, LSIRF was able to bind to the interferon-stimulated response element (ISRE) of the MHC class I promoter. The expression pattern and DNA binding activities suggest that LSIRF plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.",

keywords = "Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Blotting, Northern, Cell Line, Cloning, Molecular, DNA, DNA-Binding Proteins, Gene Expression, Interferon Regulatory Factors, Interferons, Lymphocytes, Mice, Molecular Sequence Data, Promoter Regions, Genetic, RNA, Messenger, Recombinant Proteins, Repetitive Sequences, Nucleic Acid, TATA Box, Transcription Factors",

author = "T Matsuyama and A Grossman and Mittr{\"u}cker, {H W} and Siderovski, {D P} and F Kiefer and T Kawakami and Richardson, {C D} and T Taniguchi and Yoshinaga, {S K} and Mak, {T W}",

year = "1995",

month = jun,

day = "25",

language = "English",

volume = "23",

pages = "2127--36",

journal = "NUCLEIC ACIDS RES",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "12",

}

RIS

TY - JOUR

T1 - Molecular cloning of LSIRF, a lymphoid-specific member of the interferon regulatory factor family that binds the interferon-stimulated response element (ISRE)

AU - Matsuyama, T

AU - Grossman, A

AU - Mittrücker, H W

AU - Siderovski, D P

AU - Kiefer, F

AU - Kawakami, T

AU - Richardson, C D

AU - Taniguchi, T

AU - Yoshinaga, S K

AU - Mak, T W

PY - 1995/6/25

Y1 - 1995/6/25

N2 - Interferon regulatory factor (IRF) genes encode a family of DNA-binding proteins that are involved in the transcriptional regulation of type-I interferon and/or interferon-inducible genes. We report here the characterization of LSIRF, a new member of the IRF gene family cloned from mouse spleen by the polymerase chain reaction using degenerate primers. LSIRF was found to encode a 51 kDa protein that shares a high degree of amino acid sequence homology in the DNA-binding domain with other IRF family members. LSIRF expression was detectable only in lymphoid cells. In contrast to other IRF genes, LSIRF expression was not induced by interferons, but rather by antigen-receptor mediated stimuli such as plant lectins, CD3 or IgM crosslinking. In in vitro DNA binding studies, LSIRF was able to bind to the interferon-stimulated response element (ISRE) of the MHC class I promoter. The expression pattern and DNA binding activities suggest that LSIRF plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

AB - Interferon regulatory factor (IRF) genes encode a family of DNA-binding proteins that are involved in the transcriptional regulation of type-I interferon and/or interferon-inducible genes. We report here the characterization of LSIRF, a new member of the IRF gene family cloned from mouse spleen by the polymerase chain reaction using degenerate primers. LSIRF was found to encode a 51 kDa protein that shares a high degree of amino acid sequence homology in the DNA-binding domain with other IRF family members. LSIRF expression was detectable only in lymphoid cells. In contrast to other IRF genes, LSIRF expression was not induced by interferons, but rather by antigen-receptor mediated stimuli such as plant lectins, CD3 or IgM crosslinking. In in vitro DNA binding studies, LSIRF was able to bind to the interferon-stimulated response element (ISRE) of the MHC class I promoter. The expression pattern and DNA binding activities suggest that LSIRF plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Binding Sites

KW - Blotting, Northern

KW - Cell Line

KW - Cloning, Molecular

KW - DNA

KW - DNA-Binding Proteins

KW - Gene Expression

KW - Interferon Regulatory Factors

KW - Interferons

KW - Lymphocytes

KW - Mice

KW - Molecular Sequence Data

KW - Promoter Regions, Genetic

KW - RNA, Messenger

KW - Recombinant Proteins

KW - Repetitive Sequences, Nucleic Acid

KW - TATA Box

KW - Transcription Factors

M3 - SCORING: Journal article

C2 - 7541907

VL - 23

SP - 2127

EP - 2136

JO - NUCLEIC ACIDS RES

JF - NUCLEIC ACIDS RES

SN - 0305-1048

IS - 12

ER -