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Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana

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Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana. / Wolters, Manuel; Frickmann, Hagen; Christner, Martin; Both, Anna; Rohde, Holger; Oppong, Kwabena; Akenten, Charity Wiafe; May, Jürgen; Dekker, Denise.

In: MICROORGANISMS, Vol. 8, No. 12, 21.12.2020.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wolters, M, Frickmann, H, Christner, M, Both, A, Rohde, H, Oppong, K, Akenten, CW, May, J & Dekker, D 2020, 'Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana', MICROORGANISMS, vol. 8, no. 12. https://doi.org/10.3390/microorganisms8122052

APA

Wolters, M., Frickmann, H., Christner, M., Both, A., Rohde, H., Oppong, K., Akenten, C. W., May, J., & Dekker, D. (2020). Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana. MICROORGANISMS, 8(12). https://doi.org/10.3390/microorganisms8122052

Vancouver

Wolters M, Frickmann H, Christner M, Both A, Rohde H, Oppong K et al. Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana. MICROORGANISMS. 2020 Dec 21;8(12). https://doi.org/10.3390/microorganisms8122052

Bibtex

@article{d14c8ab9eecf4c8685497a1d8a427dc6,
title = "Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana",
abstract = "BACKGROUND: Globally, Staphylococcus aureus is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance of S. aureus to locally available antibiotics is increasing but the molecular basis of resistance and the population structure of S. aureus in particular in chronic wounds are poorly described. However, this information is essential to understand the underlying mechanisms of resistance and spread of resistant clones. We therefore subjected 28 S. aureus isolates from chronic infected wounds in a rural area of Ghana to whole genome sequencing.RESULTS: Overall, resistance of S. aureus to locally available antibiotics was high and 29% were Methicillin resistant Staphylococcus aureus (MRSA). The most abundant sequence type was ST88 (29%, 8/28) followed by ST152 (18%, 5/28). All ST88 carried the mecA gene, which was associated with this sequence type only. Chloramphenicol resistance gene fexB was exclusively associated with the methicillin-resistant ST88 strains. Panton-Valentine leukocidin (PVL) carriage was associated with ST121 and ST152. Other detected mechanisms of resistance included dfrG, conferring resistance to trimethoprim.CONCLUSIONS: This study provides valuable information for understanding the population structure and resistance mechanisms of S. aureus isolated from chronic wound infections in rural Ghana.",
author = "Manuel Wolters and Hagen Frickmann and Martin Christner and Anna Both and Holger Rohde and Kwabena Oppong and Akenten, {Charity Wiafe} and J{\"u}rgen May and Denise Dekker",
year = "2020",
month = dec,
day = "21",
doi = "10.3390/microorganisms8122052",
language = "English",
volume = "8",
journal = "MICROORGANISMS",
issn = "2076-2607",
publisher = "MDPI AG",
number = "12",

}

RIS

TY - JOUR

T1 - Molecular Characterization of Staphylococcus aureus Isolated from Chronic Infected Wounds in Rural Ghana

AU - Wolters, Manuel

AU - Frickmann, Hagen

AU - Christner, Martin

AU - Both, Anna

AU - Rohde, Holger

AU - Oppong, Kwabena

AU - Akenten, Charity Wiafe

AU - May, Jürgen

AU - Dekker, Denise

PY - 2020/12/21

Y1 - 2020/12/21

N2 - BACKGROUND: Globally, Staphylococcus aureus is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance of S. aureus to locally available antibiotics is increasing but the molecular basis of resistance and the population structure of S. aureus in particular in chronic wounds are poorly described. However, this information is essential to understand the underlying mechanisms of resistance and spread of resistant clones. We therefore subjected 28 S. aureus isolates from chronic infected wounds in a rural area of Ghana to whole genome sequencing.RESULTS: Overall, resistance of S. aureus to locally available antibiotics was high and 29% were Methicillin resistant Staphylococcus aureus (MRSA). The most abundant sequence type was ST88 (29%, 8/28) followed by ST152 (18%, 5/28). All ST88 carried the mecA gene, which was associated with this sequence type only. Chloramphenicol resistance gene fexB was exclusively associated with the methicillin-resistant ST88 strains. Panton-Valentine leukocidin (PVL) carriage was associated with ST121 and ST152. Other detected mechanisms of resistance included dfrG, conferring resistance to trimethoprim.CONCLUSIONS: This study provides valuable information for understanding the population structure and resistance mechanisms of S. aureus isolated from chronic wound infections in rural Ghana.

AB - BACKGROUND: Globally, Staphylococcus aureus is an important bacterial pathogen causing a wide range of community and hospital acquired infections. In Ghana, resistance of S. aureus to locally available antibiotics is increasing but the molecular basis of resistance and the population structure of S. aureus in particular in chronic wounds are poorly described. However, this information is essential to understand the underlying mechanisms of resistance and spread of resistant clones. We therefore subjected 28 S. aureus isolates from chronic infected wounds in a rural area of Ghana to whole genome sequencing.RESULTS: Overall, resistance of S. aureus to locally available antibiotics was high and 29% were Methicillin resistant Staphylococcus aureus (MRSA). The most abundant sequence type was ST88 (29%, 8/28) followed by ST152 (18%, 5/28). All ST88 carried the mecA gene, which was associated with this sequence type only. Chloramphenicol resistance gene fexB was exclusively associated with the methicillin-resistant ST88 strains. Panton-Valentine leukocidin (PVL) carriage was associated with ST121 and ST152. Other detected mechanisms of resistance included dfrG, conferring resistance to trimethoprim.CONCLUSIONS: This study provides valuable information for understanding the population structure and resistance mechanisms of S. aureus isolated from chronic wound infections in rural Ghana.

U2 - 10.3390/microorganisms8122052

DO - 10.3390/microorganisms8122052

M3 - SCORING: Journal article

C2 - 33371449

VL - 8

JO - MICROORGANISMS

JF - MICROORGANISMS

SN - 2076-2607

IS - 12

ER -