Research ExplorerPublicationsMolecular characterisation of linezolid resistance in two va...

Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing.

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Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing. / Saager, B; Rohde, Holger; Timmerbeil, B S; Franke, Gefion; Pothmann, Werner; Dahlke, Joachim; Scherpe, Stefanie; Sobottka, Ingo; Heisig, P; Horstkotte, Matthias A.

In: EUR J CLIN MICROBIOL, Vol. 27, No. 9, 9, 2008, p. 873-878.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Saager, B, Rohde, H, Timmerbeil, BS, Franke, G, Pothmann, W, Dahlke, J, Scherpe, S, Sobottka, I, Heisig, P & Horstkotte, MA 2008, 'Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing.', EUR J CLIN MICROBIOL, vol. 27, no. 9, 9, pp. 873-878. <http://www.ncbi.nlm.nih.gov/pubmed/18421487?dopt=Citation>

APA

Saager, B., Rohde, H., Timmerbeil, B. S., Franke, G., Pothmann, W., Dahlke, J., Scherpe, S., Sobottka, I., Heisig, P., & Horstkotte, M. A. (2008). Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing. EUR J CLIN MICROBIOL, 27(9), 873-878. [9]. http://www.ncbi.nlm.nih.gov/pubmed/18421487?dopt=Citation

Vancouver

Saager B, Rohde H, Timmerbeil BS, Franke G, Pothmann W, Dahlke J et al. Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing. EUR J CLIN MICROBIOL. 2008;27(9):873-878. 9.

Bibtex

@article{488e014d41da46e3a84fee7c69402a8f,
title = "Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing.",
abstract = "In this paper, we describe the phenotypic and molecular characteristics of two clinically relevant, vancomycin-resistant (VanB), linezolid-resistant Enterococcus faecium isolates. Pyrosequencing showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was used to quantify the proportion of G to T mutations among six different 23S rRNA genes in E. faecium as a marker for the molecular level of resistance to linezolid. In both isolates, the G to T mutation was found in two of six alleles, and no further mutations in the genes coding for 23S rRNA were found. The dynamic process of linezolid resistance could be demonstrated by the complete reversion of resistant alleles back to only wild type alleles in consecutive isolates of one isolate. Pyrosequencing being used to detect and quantify resistance to linezolid has been proven as a fast and reliable molecular screening method for monitoring linezolid resistance.",
author = "B Saager and Holger Rohde and Timmerbeil, {B S} and Gefion Franke and Werner Pothmann and Joachim Dahlke and Stefanie Scherpe and Ingo Sobottka and P Heisig and Horstkotte, {Matthias A.}",
year = "2008",
language = "Deutsch",
volume = "27",
pages = "873--878",
journal = "EUR J CLIN MICROBIOL",
issn = "0934-9723",
publisher = "Springer",
number = "9",

}

RIS

TY - JOUR

T1 - Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing.

AU - Saager, B

AU - Rohde, Holger

AU - Timmerbeil, B S

AU - Franke, Gefion

AU - Pothmann, Werner

AU - Dahlke, Joachim

AU - Scherpe, Stefanie

AU - Sobottka, Ingo

AU - Heisig, P

AU - Horstkotte, Matthias A.

PY - 2008

Y1 - 2008

N2 - In this paper, we describe the phenotypic and molecular characteristics of two clinically relevant, vancomycin-resistant (VanB), linezolid-resistant Enterococcus faecium isolates. Pyrosequencing showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was used to quantify the proportion of G to T mutations among six different 23S rRNA genes in E. faecium as a marker for the molecular level of resistance to linezolid. In both isolates, the G to T mutation was found in two of six alleles, and no further mutations in the genes coding for 23S rRNA were found. The dynamic process of linezolid resistance could be demonstrated by the complete reversion of resistant alleles back to only wild type alleles in consecutive isolates of one isolate. Pyrosequencing being used to detect and quantify resistance to linezolid has been proven as a fast and reliable molecular screening method for monitoring linezolid resistance.

AB - In this paper, we describe the phenotypic and molecular characteristics of two clinically relevant, vancomycin-resistant (VanB), linezolid-resistant Enterococcus faecium isolates. Pyrosequencing showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was used to quantify the proportion of G to T mutations among six different 23S rRNA genes in E. faecium as a marker for the molecular level of resistance to linezolid. In both isolates, the G to T mutation was found in two of six alleles, and no further mutations in the genes coding for 23S rRNA were found. The dynamic process of linezolid resistance could be demonstrated by the complete reversion of resistant alleles back to only wild type alleles in consecutive isolates of one isolate. Pyrosequencing being used to detect and quantify resistance to linezolid has been proven as a fast and reliable molecular screening method for monitoring linezolid resistance.

M3 - SCORING: Zeitschriftenaufsatz

VL - 27

SP - 873

EP - 878

JO - EUR J CLIN MICROBIOL

JF - EUR J CLIN MICROBIOL

SN - 0934-9723

IS - 9

M1 - 9

ER -