Modified sleeve anastomosis for reconstruction of the hepatic artery in rat liver transplantation

TY - JOUR

T1 - Modified sleeve anastomosis for reconstruction of the hepatic artery in rat liver transplantation

AU - Li, Jun

AU - Dahmen, Uta

AU - Dirsch, Olaf

AU - Shen, Kai

AU - Gu, Yanli

AU - Broelsch, Christoph Erich

N1 - Copyright 2002 Wiley-Liss, Inc.

PY - 2002

Y1 - 2002

N2 - End-to-end sleeve anastomosis between a donor common hepatic artery and a recipient proper hepatic artery was proven to be the most physiological and simple method for hepatic rearterialization in rat liver transplantation. Current technical variants of the sleeve technique, however, are hampered by the high rate of bleeding from the anastomotic site. This report deals with a technical modification which inhibits postoperative bleeding efficiently. The procedure consisted of a guiding suture, as previously described in other technical variants, and a modified fixing suture. Instead of using a single stitch to fix the feeding vessel with the receiving vessel, a running suture between the edge of the donor common hepatic artery and the adventitia of the recipient proper hepatic artery was performed to avoid a possible backflow. The patency rate of 91% was as high as reported by others using a sleeve technique, which was also reflected in the histomorphological picture, being indistinguishable from normal liver histology. This technical modification simplified the procedure of reconstructing the hepatic artery and could contribute to a wider use of the arterialized liver transplantation model in rats.

AB - End-to-end sleeve anastomosis between a donor common hepatic artery and a recipient proper hepatic artery was proven to be the most physiological and simple method for hepatic rearterialization in rat liver transplantation. Current technical variants of the sleeve technique, however, are hampered by the high rate of bleeding from the anastomotic site. This report deals with a technical modification which inhibits postoperative bleeding efficiently. The procedure consisted of a guiding suture, as previously described in other technical variants, and a modified fixing suture. Instead of using a single stitch to fix the feeding vessel with the receiving vessel, a running suture between the edge of the donor common hepatic artery and the adventitia of the recipient proper hepatic artery was performed to avoid a possible backflow. The patency rate of 91% was as high as reported by others using a sleeve technique, which was also reflected in the histomorphological picture, being indistinguishable from normal liver histology. This technical modification simplified the procedure of reconstructing the hepatic artery and could contribute to a wider use of the arterialized liver transplantation model in rats.

KW - Anastomosis, Surgical

KW - Animals

KW - Biopsy, Needle

KW - Disease Models, Animal

KW - Graft Survival

KW - Hepatic Artery

KW - Liver Transplantation

KW - Male

KW - Microsurgery

KW - Rats

KW - Rats, Inbred Lew

KW - Reconstructive Surgical Procedures

KW - Risk Assessment

KW - Sensitivity and Specificity

KW - Suture Techniques

KW - Treatment Outcome

KW - Vascular Patency

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 11921073

VL - 22

SP - 62

EP - 68

JO - MICROSURG

JF - MICROSURG

SN - 0738-1085

IS - 2

ER -