Modified oligonucleotides as tools for allele-specific amplification.
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Modified oligonucleotides as tools for allele-specific amplification. / Strerath, Michael; Detmer, Ilka; Gaster, Jens; Marx, Andreas.
In: Methods Mol Biol, Vol. 402, 2007, p. 317-328.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Modified oligonucleotides as tools for allele-specific amplification.
AU - Strerath, Michael
AU - Detmer, Ilka
AU - Gaster, Jens
AU - Marx, Andreas
PY - 2007
Y1 - 2007
N2 - Allele-specific polymerase chain reaction (PCR), a method that reports nucleotide variations through either the presence or the absence of a DNA product obtained through PCR amplification, holds the promise to combine target amplification and analysis in one single step. Recently, it has been reported that the selectivity of allele-specific PCR can be significantly increased through the employment of chemically modified primer probes. Here, we report on significant developments of primer probe design and synthesis along this line.
AB - Allele-specific polymerase chain reaction (PCR), a method that reports nucleotide variations through either the presence or the absence of a DNA product obtained through PCR amplification, holds the promise to combine target amplification and analysis in one single step. Recently, it has been reported that the selectivity of allele-specific PCR can be significantly increased through the employment of chemically modified primer probes. Here, we report on significant developments of primer probe design and synthesis along this line.
M3 - SCORING: Zeitschriftenaufsatz
VL - 402
SP - 317
EP - 328
JO - Methods Mol Biol
JF - Methods Mol Biol
SN - 1064-3745
ER -