Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
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Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. / Dominici, M; Le Blanc, K; Mueller, I; Slaper-Cortenbach, I; Marini, Fc; Krause, Ds; Deans, Rj; Keating, A; Prockop, Dj; Horwitz, Em.
In: CYTOTHERAPY, Vol. 8, No. 4, 2006, p. 315-357.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
AU - Dominici, M
AU - Le Blanc, K
AU - Mueller, I
AU - Slaper-Cortenbach, I
AU - Marini, Fc
AU - Krause, Ds
AU - Deans, Rj
AU - Keating, A
AU - Prockop, Dj
AU - Horwitz, Em
PY - 2006
Y1 - 2006
N2 - The considerable therapeutic potential of human multipotent mesenchymal stromal cells (MSC) has generated markedly increasing interest in a wide variety of biomedical disciplines. However, investigators report studies of MSC using different methods of isolation and expansion, and different approaches to characterizing the cells. Thus it is increasingly difficult to compare and contrast study outcomes, which hinders progress in the field. To begin to address this issue, the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy proposes minimal criteria to define human MSC. First, MSC must be plastic-adherent when maintained in standard culture conditions. Second, MSC must express CD105, CD73 and CD90, and lack expression of CD45, CD34, CD14 or CD11b, CD79alpha or CD19 and HLA-DR surface molecules. Third, MSC must differentiate to osteoblasts, adipocytes and chondroblasts in vitro. While these criteria will probably require modification as new knowledge unfolds, we believe this minimal set of standard criteria will foster a more uniform characterization of MSC and facilitate the exchange of data among investigators.
AB - The considerable therapeutic potential of human multipotent mesenchymal stromal cells (MSC) has generated markedly increasing interest in a wide variety of biomedical disciplines. However, investigators report studies of MSC using different methods of isolation and expansion, and different approaches to characterizing the cells. Thus it is increasingly difficult to compare and contrast study outcomes, which hinders progress in the field. To begin to address this issue, the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy proposes minimal criteria to define human MSC. First, MSC must be plastic-adherent when maintained in standard culture conditions. Second, MSC must express CD105, CD73 and CD90, and lack expression of CD45, CD34, CD14 or CD11b, CD79alpha or CD19 and HLA-DR surface molecules. Third, MSC must differentiate to osteoblasts, adipocytes and chondroblasts in vitro. While these criteria will probably require modification as new knowledge unfolds, we believe this minimal set of standard criteria will foster a more uniform characterization of MSC and facilitate the exchange of data among investigators.
KW - Antigens, CD
KW - Cell Culture Techniques
KW - Cell Differentiation
KW - Cell- and Tissue-Based Therapy
KW - Humans
KW - Mesenchymal Stromal Cells
KW - Multipotent Stem Cells
KW - Stromal Cells
U2 - 10.1080/14653240600855905
DO - 10.1080/14653240600855905
M3 - SCORING: Journal article
C2 - 16923606
VL - 8
SP - 315
EP - 357
JO - CYTOTHERAPY
JF - CYTOTHERAPY
SN - 1465-3249
IS - 4
ER -
