MINFLUX imaging of a bacterial molecular machine at nanometer resolution

Alexander Carsten; Maren Rudolph; Tobias Weihs; Roman Schmidt; Isabelle Jansen; Christian A Wurm; Andreas Diepold; Antonio Virgilio Failla; Manuel Wolters; Martin Aepfelbacher

doi:10.1088/2050-6120/aca880

MINFLUX imaging of a bacterial molecular machine at nanometer resolution

Standard

MINFLUX imaging of a bacterial molecular machine at nanometer resolution. / Carsten, Alexander; Rudolph, Maren; Weihs, Tobias; Schmidt, Roman; Jansen, Isabelle; Wurm, Christian A; Diepold, Andreas; Failla, Antonio Virgilio ; Wolters, Manuel ; Aepfelbacher, Martin.

In: METHODS APPL FLUORES, Vol. 11, No. 1, 015004, 01.2023.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Carsten, A, Rudolph, M, Weihs, T, Schmidt, R, Jansen, I, Wurm, CA, Diepold, A, Failla, AV , Wolters, M & Aepfelbacher, M 2023, 'MINFLUX imaging of a bacterial molecular machine at nanometer resolution', METHODS APPL FLUORES, vol. 11, no. 1, 015004. https://doi.org/10.1088/2050-6120/aca880

APA

Carsten, A., Rudolph, M., Weihs, T., Schmidt, R., Jansen, I., Wurm, C. A., Diepold, A., Failla, A. V., Wolters, M., & Aepfelbacher, M. (2023). MINFLUX imaging of a bacterial molecular machine at nanometer resolution. METHODS APPL FLUORES, 11(1), [015004]. https://doi.org/10.1088/2050-6120/aca880

Vancouver

Carsten A, Rudolph M, Weihs T, Schmidt R, Jansen I, Wurm CA et al. MINFLUX imaging of a bacterial molecular machine at nanometer resolution. METHODS APPL FLUORES. 2023 Jan;11(1). 015004. https://doi.org/10.1088/2050-6120/aca880

Bibtex

@article{68bf0fd6fcea4421b57f3eaf5366541e,

title = "MINFLUX imaging of a bacterial molecular machine at nanometer resolution",

abstract = "The resolution achievable with the established super-resolution fluorescence nanoscopy methods, such as STORM or STED, is in general not sufficient to resolve protein complexes or even individual proteins. Recently, minimal photon flux (MINFLUX) nanoscopy has been introduced that combines the strengths of STED and STORM nanoscopy and can achieve a localization precision of less than 5 nm. We established a generally applicable workflow for MINFLUX imaging and applied it for the first time to a bacterial molecular machinein situ, i.e., the injectisome of the enteropathogenY. enterocolitica. We demonstrate with a pore protein of the injectisome that MINFLUX can achieve a resolution down to the single molecule levelin situ. By imaging a sorting platform protein using 3D-MINFLUX, insights into the precise localization and distribution of an injectisome component in a bacterial cell could be accomplished. MINFLUX nanoscopy has the potential to revolutionize super-resolution imaging of dynamic molecular processes in bacteria and eukaryotes.",

keywords = "Microscopy, Fluorescence/methods, Bacteria",

author = "Alexander Carsten and Maren Rudolph and Tobias Weihs and Roman Schmidt and Isabelle Jansen and Wurm, {Christian A} and Andreas Diepold and Failla, {Antonio Virgilio} and Manuel Wolters and Martin Aepfelbacher",

note = "{\textcopyright} 2022 IOP Publishing Ltd.",

year = "2023",

month = jan,

doi = "10.1088/2050-6120/aca880",

language = "English",

volume = "11",

journal = "METHODS APPL FLUORES",

issn = "2050-6120",

publisher = "IOP Publishing Ltd.",

number = "1",

}

RIS

TY - JOUR

T1 - MINFLUX imaging of a bacterial molecular machine at nanometer resolution

AU - Carsten, Alexander

AU - Rudolph, Maren

AU - Weihs, Tobias

AU - Schmidt, Roman

AU - Jansen, Isabelle

AU - Wurm, Christian A

AU - Diepold, Andreas

AU - Failla, Antonio Virgilio

AU - Wolters, Manuel

AU - Aepfelbacher, Martin

PY - 2023/1

Y1 - 2023/1

N2 - The resolution achievable with the established super-resolution fluorescence nanoscopy methods, such as STORM or STED, is in general not sufficient to resolve protein complexes or even individual proteins. Recently, minimal photon flux (MINFLUX) nanoscopy has been introduced that combines the strengths of STED and STORM nanoscopy and can achieve a localization precision of less than 5 nm. We established a generally applicable workflow for MINFLUX imaging and applied it for the first time to a bacterial molecular machinein situ, i.e., the injectisome of the enteropathogenY. enterocolitica. We demonstrate with a pore protein of the injectisome that MINFLUX can achieve a resolution down to the single molecule levelin situ. By imaging a sorting platform protein using 3D-MINFLUX, insights into the precise localization and distribution of an injectisome component in a bacterial cell could be accomplished. MINFLUX nanoscopy has the potential to revolutionize super-resolution imaging of dynamic molecular processes in bacteria and eukaryotes.

AB - The resolution achievable with the established super-resolution fluorescence nanoscopy methods, such as STORM or STED, is in general not sufficient to resolve protein complexes or even individual proteins. Recently, minimal photon flux (MINFLUX) nanoscopy has been introduced that combines the strengths of STED and STORM nanoscopy and can achieve a localization precision of less than 5 nm. We established a generally applicable workflow for MINFLUX imaging and applied it for the first time to a bacterial molecular machinein situ, i.e., the injectisome of the enteropathogenY. enterocolitica. We demonstrate with a pore protein of the injectisome that MINFLUX can achieve a resolution down to the single molecule levelin situ. By imaging a sorting platform protein using 3D-MINFLUX, insights into the precise localization and distribution of an injectisome component in a bacterial cell could be accomplished. MINFLUX nanoscopy has the potential to revolutionize super-resolution imaging of dynamic molecular processes in bacteria and eukaryotes.

KW - Microscopy, Fluorescence/methods

KW - Bacteria

U2 - 10.1088/2050-6120/aca880

DO - 10.1088/2050-6120/aca880

M3 - SCORING: Journal article

C2 - 36541558

VL - 11

JO - METHODS APPL FLUORES

JF - METHODS APPL FLUORES

SN - 2050-6120

IS - 1

M1 - 015004

ER -