Research ExplorerPublicationsMicroRNA signatures in chemotherapy resistant esophageal can...

MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines

Standard

MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines. / Hummel, Richard; Sie, Corina; Watson, David I; Wang, Tingting; Ansar, Alfiya; Michael, Michael Z; Van der Hoek, Mark; Haier, Joerg; Hussey, Damian J.

In: WORLD J GASTROENTERO, Vol. 20, No. 40, 28.10.2014, p. 14904-12.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hummel, R, Sie, C, Watson, DI, Wang, T, Ansar, A, Michael, MZ, Van der Hoek, M, Haier, J & Hussey, DJ 2014, 'MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines', WORLD J GASTROENTERO, vol. 20, no. 40, pp. 14904-12. https://doi.org/10.3748/wjg.v20.i40.14904

APA

Hummel, R., Sie, C., Watson, D. I., Wang, T., Ansar, A., Michael, M. Z., Van der Hoek, M., Haier, J., & Hussey, D. J. (2014). MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines. WORLD J GASTROENTERO, 20(40), 14904-12. https://doi.org/10.3748/wjg.v20.i40.14904

Vancouver

Hummel R, Sie C, Watson DI, Wang T, Ansar A, Michael MZ et al. MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines. WORLD J GASTROENTERO. 2014 Oct 28;20(40):14904-12. https://doi.org/10.3748/wjg.v20.i40.14904

Bibtex

@article{02d20f0ef8a44faa9a22526a7d4ea849,
title = "MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines",
abstract = "AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines.METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated.RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs.CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.",
keywords = "Adenocarcinoma, Antineoplastic Agents, Biomarkers, Tumor, Carcinoma, Squamous Cell, Cell Line, Tumor, Cell Survival, Cisplatin, Drug Resistance, Neoplasm, Esophageal Neoplasms, Fluorouracil, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, MicroRNAs, Oligonucleotide Array Sequence Analysis, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction",
author = "Richard Hummel and Corina Sie and Watson, {David I} and Tingting Wang and Alfiya Ansar and Michael, {Michael Z} and {Van der Hoek}, Mark and Joerg Haier and Hussey, {Damian J}",
year = "2014",
month = oct,
day = "28",
doi = "10.3748/wjg.v20.i40.14904",
language = "English",
volume = "20",
pages = "14904--12",
journal = "WORLD J GASTROENTERO",
issn = "1007-9327",
publisher = "WJG Press",
number = "40",

}

RIS

TY - JOUR

T1 - MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines

AU - Hummel, Richard

AU - Sie, Corina

AU - Watson, David I

AU - Wang, Tingting

AU - Ansar, Alfiya

AU - Michael, Michael Z

AU - Van der Hoek, Mark

AU - Haier, Joerg

AU - Hussey, Damian J

PY - 2014/10/28

Y1 - 2014/10/28

N2 - AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines.METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated.RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs.CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.

AB - AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines.METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated.RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs.CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.

KW - Adenocarcinoma

KW - Antineoplastic Agents

KW - Biomarkers, Tumor

KW - Carcinoma, Squamous Cell

KW - Cell Line, Tumor

KW - Cell Survival

KW - Cisplatin

KW - Drug Resistance, Neoplasm

KW - Esophageal Neoplasms

KW - Fluorouracil

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Neoplastic

KW - Humans

KW - MicroRNAs

KW - Oligonucleotide Array Sequence Analysis

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.3748/wjg.v20.i40.14904

DO - 10.3748/wjg.v20.i40.14904

M3 - SCORING: Journal article

C2 - 25356050

VL - 20

SP - 14904

EP - 14912

JO - WORLD J GASTROENTERO

JF - WORLD J GASTROENTERO

SN - 1007-9327

IS - 40

ER -