MicroRNA profiling using fluorescence-labeled beads: data acquisition and processing.
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MicroRNA profiling using fluorescence-labeled beads: data acquisition and processing. / Streichert, Thomas; Otto, Benjamin; Lehmann, Ulrich.
In: Methods Mol Biol, Vol. 676, 2011, p. 253-268.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - MicroRNA profiling using fluorescence-labeled beads: data acquisition and processing.
AU - Streichert, Thomas
AU - Otto, Benjamin
AU - Lehmann, Ulrich
PY - 2011
Y1 - 2011
N2 - The discovery of small regulatory RNA molecules during the last years has changed our understanding of many biological and pathological processes. The most prominent and best analyzed class of these small regulatory noncoding RNAs is composed of the microRNAs. The analysis of microRNA expression patterns is now widely used in biology and pathology employing a range of methodologies. However, procedures for data processing and calculations are far from standardized and differ considerably between published studies. This makes comparisons and meta-analyses still quite difficult. In this chapter, we describe a modified method for normalization and processing of raw data obtained if utilizing fluorescence-labeled bead technology from Luminex. Inc.
AB - The discovery of small regulatory RNA molecules during the last years has changed our understanding of many biological and pathological processes. The most prominent and best analyzed class of these small regulatory noncoding RNAs is composed of the microRNAs. The analysis of microRNA expression patterns is now widely used in biology and pathology employing a range of methodologies. However, procedures for data processing and calculations are far from standardized and differ considerably between published studies. This makes comparisons and meta-analyses still quite difficult. In this chapter, we describe a modified method for normalization and processing of raw data obtained if utilizing fluorescence-labeled bead technology from Luminex. Inc.
M3 - SCORING: Zeitschriftenaufsatz
VL - 676
SP - 253
EP - 268
JO - Methods Mol Biol
JF - Methods Mol Biol
SN - 1064-3745
ER -