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Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii

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Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii. / Frangoulidis, Dimitrios; Splettstoesser, Wolf D; Landt, Olfert; Dehnhardt, Jasmin; Henning, Klaus; Hilbert, Angela; Bauer, Tilman; Antwerpen, Markus; Meyer, Hermann; Walter, Mathias C; Knobloch, Johannes K-M.

In: PLOS ONE, Vol. 8, No. 1, 2013, p. e53440.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Frangoulidis, D, Splettstoesser, WD, Landt, O, Dehnhardt, J, Henning, K, Hilbert, A, Bauer, T, Antwerpen, M, Meyer, H, Walter, MC & Knobloch, JK-M 2013, 'Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii', PLOS ONE, vol. 8, no. 1, pp. e53440. https://doi.org/10.1371/journal.pone.0053440

APA

Frangoulidis, D., Splettstoesser, W. D., Landt, O., Dehnhardt, J., Henning, K., Hilbert, A., Bauer, T., Antwerpen, M., Meyer, H., Walter, M. C., & Knobloch, J. K-M. (2013). Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii. PLOS ONE, 8(1), e53440. https://doi.org/10.1371/journal.pone.0053440

Vancouver

Frangoulidis D, Splettstoesser WD, Landt O, Dehnhardt J, Henning K, Hilbert A et al. Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii. PLOS ONE. 2013;8(1):e53440. https://doi.org/10.1371/journal.pone.0053440

Bibtex

@article{4d394c3f275c4deda1caf487bb5adb6c,
title = "Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii",
abstract = "The acute disease antigen A (adaA) gene is believed to be associated with Coxiella burnetii strains causing acute Q fever. The detailed analysis of the adaA genomic region of 23 human- and 86 animal-derived C. burnetii isolates presented in this study reveals a much more polymorphic appearance and distribution of the adaA gene, resulting in a classification of C. burnetii strains of better differentiation than previously anticipated. Three different genomic variants of the adaA gene were identified which could be detected in isolates from acute and chronic patients, rendering the association of adaA positive strains with acute Q fever disease disputable. In addition, all adaA positive strains in humans and animals showed the occurrence of the QpH1 plasmid. All adaA positive isolates of acute human patients except one showed a distinct SNP variation at position 431, also predominant in sheep strains, which correlates well with the observation that sheep are a major source of human infection. Furthermore, the phylogenetic analysis of the adaA gene revealed three deletion events and supported the hypothesis that strain Dugway 5J108-111 might be the ancestor of all known C. burnetii strains. Based on our findings, we could confirm the QpDV group and we were able to define a new genotypic cluster. The adaA gene polymorphisms shown here improve molecular typing of Q fever, and give new insights into microevolutionary adaption processes in C. burnetii.",
keywords = "Antigens, Bacterial, Bacterial Outer Membrane Proteins, Bacterial Proteins, Chromosomes, Bacterial, Coxiella burnetii, DNA Primers, DNA, Bacterial, Evolution, Molecular, Gene Deletion, Genetic Markers, Genotype, Humans, Molecular Typing, Phylogeny, Polymorphism, Genetic, Proportional Hazards Models, Q Fever, Real-Time Polymerase Chain Reaction, Journal Article, Research Support, Non-U.S. Gov't",
author = "Dimitrios Frangoulidis and Splettstoesser, {Wolf D} and Olfert Landt and Jasmin Dehnhardt and Klaus Henning and Angela Hilbert and Tilman Bauer and Markus Antwerpen and Hermann Meyer and Walter, {Mathias C} and Knobloch, {Johannes K-M}",
year = "2013",
doi = "10.1371/journal.pone.0053440",
language = "English",
volume = "8",
pages = "e53440",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

RIS

TY - JOUR

T1 - Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii

AU - Frangoulidis, Dimitrios

AU - Splettstoesser, Wolf D

AU - Landt, Olfert

AU - Dehnhardt, Jasmin

AU - Henning, Klaus

AU - Hilbert, Angela

AU - Bauer, Tilman

AU - Antwerpen, Markus

AU - Meyer, Hermann

AU - Walter, Mathias C

AU - Knobloch, Johannes K-M

PY - 2013

Y1 - 2013

N2 - The acute disease antigen A (adaA) gene is believed to be associated with Coxiella burnetii strains causing acute Q fever. The detailed analysis of the adaA genomic region of 23 human- and 86 animal-derived C. burnetii isolates presented in this study reveals a much more polymorphic appearance and distribution of the adaA gene, resulting in a classification of C. burnetii strains of better differentiation than previously anticipated. Three different genomic variants of the adaA gene were identified which could be detected in isolates from acute and chronic patients, rendering the association of adaA positive strains with acute Q fever disease disputable. In addition, all adaA positive strains in humans and animals showed the occurrence of the QpH1 plasmid. All adaA positive isolates of acute human patients except one showed a distinct SNP variation at position 431, also predominant in sheep strains, which correlates well with the observation that sheep are a major source of human infection. Furthermore, the phylogenetic analysis of the adaA gene revealed three deletion events and supported the hypothesis that strain Dugway 5J108-111 might be the ancestor of all known C. burnetii strains. Based on our findings, we could confirm the QpDV group and we were able to define a new genotypic cluster. The adaA gene polymorphisms shown here improve molecular typing of Q fever, and give new insights into microevolutionary adaption processes in C. burnetii.

AB - The acute disease antigen A (adaA) gene is believed to be associated with Coxiella burnetii strains causing acute Q fever. The detailed analysis of the adaA genomic region of 23 human- and 86 animal-derived C. burnetii isolates presented in this study reveals a much more polymorphic appearance and distribution of the adaA gene, resulting in a classification of C. burnetii strains of better differentiation than previously anticipated. Three different genomic variants of the adaA gene were identified which could be detected in isolates from acute and chronic patients, rendering the association of adaA positive strains with acute Q fever disease disputable. In addition, all adaA positive strains in humans and animals showed the occurrence of the QpH1 plasmid. All adaA positive isolates of acute human patients except one showed a distinct SNP variation at position 431, also predominant in sheep strains, which correlates well with the observation that sheep are a major source of human infection. Furthermore, the phylogenetic analysis of the adaA gene revealed three deletion events and supported the hypothesis that strain Dugway 5J108-111 might be the ancestor of all known C. burnetii strains. Based on our findings, we could confirm the QpDV group and we were able to define a new genotypic cluster. The adaA gene polymorphisms shown here improve molecular typing of Q fever, and give new insights into microevolutionary adaption processes in C. burnetii.

KW - Antigens, Bacterial

KW - Bacterial Outer Membrane Proteins

KW - Bacterial Proteins

KW - Chromosomes, Bacterial

KW - Coxiella burnetii

KW - DNA Primers

KW - DNA, Bacterial

KW - Evolution, Molecular

KW - Gene Deletion

KW - Genetic Markers

KW - Genotype

KW - Humans

KW - Molecular Typing

KW - Phylogeny

KW - Polymorphism, Genetic

KW - Proportional Hazards Models

KW - Q Fever

KW - Real-Time Polymerase Chain Reaction

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1371/journal.pone.0053440

DO - 10.1371/journal.pone.0053440

M3 - SCORING: Journal article

C2 - 23301072

VL - 8

SP - e53440

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 1

ER -