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Metastatic tumor cell arrest in the liver-lumen occlusion and specific adhesion are not exclusive

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Metastatic tumor cell arrest in the liver-lumen occlusion and specific adhesion are not exclusive. / Gassmann, Peter; Hemping-Bovenkerk, Andre; Mees, Soeren Torge; Haier, Joerg.

In: INT J COLORECTAL DIS, Vol. 24, No. 7, 07.2009, p. 851-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Gassmann, P, Hemping-Bovenkerk, A, Mees, ST & Haier, J 2009, 'Metastatic tumor cell arrest in the liver-lumen occlusion and specific adhesion are not exclusive', INT J COLORECTAL DIS, vol. 24, no. 7, pp. 851-8. https://doi.org/10.1007/s00384-009-0694-2

APA

Gassmann, P., Hemping-Bovenkerk, A., Mees, S. T., & Haier, J. (2009). Metastatic tumor cell arrest in the liver-lumen occlusion and specific adhesion are not exclusive. INT J COLORECTAL DIS, 24(7), 851-8. https://doi.org/10.1007/s00384-009-0694-2

Vancouver

Gassmann P, Hemping-Bovenkerk A, Mees ST, Haier J. Metastatic tumor cell arrest in the liver-lumen occlusion and specific adhesion are not exclusive. INT J COLORECTAL DIS. 2009 Jul;24(7):851-8. https://doi.org/10.1007/s00384-009-0694-2

Bibtex

@article{43e484b7f5844448b8532cd2b60a2933,
title = "Metastatic tumor cell arrest in the liver-lumen occlusion and specific adhesion are not exclusive",
abstract = "INTRODUCTION: In metastasis research, modern microscopic techniques shed a new light on the mechanisms of metastatic tumor cell arrest in the microcirculation of potential metastasis target organs. In this study, we differentiated the contribution of mechanical cell arrest, determined as lumen occlusion of liver sinusoids by tumor cells, and specific cell adhesion mediated by integrins for the arrest of human colon cancer cells in rat livers.MATERIALS AND METHODS: Using in vivo microscopy, the diameters of liver sinusoids of two different rat strains (CD, 250-300 g and RNU, 80-120 g) were determined. Cells (HT-29LMM) were intracardially injected, and the numbers of arrested cells and the rates of sinusoid occluding cells were determined.RESULTS: Mean sinusoid diameter in CD rats was 6.98 +/- 1.42 microm compared to 5.14 +/- 1.11 microm in RNU rats (p < 0.001). The numbers of arrested tumor cells and the rates of extravasated tumor cells did not differ between the two rat strains. Nevertheless, 5 and 30 min after cell injection, 35 +/- 15% and 19 +/- 8% of arrested cells, respectively, appeared lumen occluding in RNU rats and 9 +/- 6% and 3 +/- 3%, respectively, in CD rats (p < 0.05). Despite the higher rates of lumen occlusive cells in RNU rats, inhibition of beta-1 or beta-4 integrins significantly impaired cell arrest by 30-60% in both strains.DISCUSSION: In summary, these results demonstrate that lumen occlusion alone, as determined by in vivo microscopy, is insufficient to establish stable tumor cell arrest of colon carcinoma cells in metastatic target organs and does therefore not rule out the requirement of specific adhesive interactions for tumor cell arrest in the microcirculation.",
keywords = "Animals, Cell Adhesion, Cell Line, Tumor, Colonic Neoplasms, Hepatic Veno-Occlusive Disease, Humans, Integrins, Liver, Male, Microscopy, Fluorescence, Neoplasm Metastasis, Rats, Rats, Sprague-Dawley",
author = "Peter Gassmann and Andre Hemping-Bovenkerk and Mees, {Soeren Torge} and Joerg Haier",
year = "2009",
month = jul,
doi = "10.1007/s00384-009-0694-2",
language = "English",
volume = "24",
pages = "851--8",
journal = "INT J COLORECTAL DIS",
issn = "0179-1958",
publisher = "Springer",
number = "7",

}

RIS

TY - JOUR

T1 - Metastatic tumor cell arrest in the liver-lumen occlusion and specific adhesion are not exclusive

AU - Gassmann, Peter

AU - Hemping-Bovenkerk, Andre

AU - Mees, Soeren Torge

AU - Haier, Joerg

PY - 2009/7

Y1 - 2009/7

N2 - INTRODUCTION: In metastasis research, modern microscopic techniques shed a new light on the mechanisms of metastatic tumor cell arrest in the microcirculation of potential metastasis target organs. In this study, we differentiated the contribution of mechanical cell arrest, determined as lumen occlusion of liver sinusoids by tumor cells, and specific cell adhesion mediated by integrins for the arrest of human colon cancer cells in rat livers.MATERIALS AND METHODS: Using in vivo microscopy, the diameters of liver sinusoids of two different rat strains (CD, 250-300 g and RNU, 80-120 g) were determined. Cells (HT-29LMM) were intracardially injected, and the numbers of arrested cells and the rates of sinusoid occluding cells were determined.RESULTS: Mean sinusoid diameter in CD rats was 6.98 +/- 1.42 microm compared to 5.14 +/- 1.11 microm in RNU rats (p < 0.001). The numbers of arrested tumor cells and the rates of extravasated tumor cells did not differ between the two rat strains. Nevertheless, 5 and 30 min after cell injection, 35 +/- 15% and 19 +/- 8% of arrested cells, respectively, appeared lumen occluding in RNU rats and 9 +/- 6% and 3 +/- 3%, respectively, in CD rats (p < 0.05). Despite the higher rates of lumen occlusive cells in RNU rats, inhibition of beta-1 or beta-4 integrins significantly impaired cell arrest by 30-60% in both strains.DISCUSSION: In summary, these results demonstrate that lumen occlusion alone, as determined by in vivo microscopy, is insufficient to establish stable tumor cell arrest of colon carcinoma cells in metastatic target organs and does therefore not rule out the requirement of specific adhesive interactions for tumor cell arrest in the microcirculation.

AB - INTRODUCTION: In metastasis research, modern microscopic techniques shed a new light on the mechanisms of metastatic tumor cell arrest in the microcirculation of potential metastasis target organs. In this study, we differentiated the contribution of mechanical cell arrest, determined as lumen occlusion of liver sinusoids by tumor cells, and specific cell adhesion mediated by integrins for the arrest of human colon cancer cells in rat livers.MATERIALS AND METHODS: Using in vivo microscopy, the diameters of liver sinusoids of two different rat strains (CD, 250-300 g and RNU, 80-120 g) were determined. Cells (HT-29LMM) were intracardially injected, and the numbers of arrested cells and the rates of sinusoid occluding cells were determined.RESULTS: Mean sinusoid diameter in CD rats was 6.98 +/- 1.42 microm compared to 5.14 +/- 1.11 microm in RNU rats (p < 0.001). The numbers of arrested tumor cells and the rates of extravasated tumor cells did not differ between the two rat strains. Nevertheless, 5 and 30 min after cell injection, 35 +/- 15% and 19 +/- 8% of arrested cells, respectively, appeared lumen occluding in RNU rats and 9 +/- 6% and 3 +/- 3%, respectively, in CD rats (p < 0.05). Despite the higher rates of lumen occlusive cells in RNU rats, inhibition of beta-1 or beta-4 integrins significantly impaired cell arrest by 30-60% in both strains.DISCUSSION: In summary, these results demonstrate that lumen occlusion alone, as determined by in vivo microscopy, is insufficient to establish stable tumor cell arrest of colon carcinoma cells in metastatic target organs and does therefore not rule out the requirement of specific adhesive interactions for tumor cell arrest in the microcirculation.

KW - Animals

KW - Cell Adhesion

KW - Cell Line, Tumor

KW - Colonic Neoplasms

KW - Hepatic Veno-Occlusive Disease

KW - Humans

KW - Integrins

KW - Liver

KW - Male

KW - Microscopy, Fluorescence

KW - Neoplasm Metastasis

KW - Rats

KW - Rats, Sprague-Dawley

U2 - 10.1007/s00384-009-0694-2

DO - 10.1007/s00384-009-0694-2

M3 - SCORING: Journal article

C2 - 19319542

VL - 24

SP - 851

EP - 858

JO - INT J COLORECTAL DIS

JF - INT J COLORECTAL DIS

SN - 0179-1958

IS - 7

ER -