Merkel cell polyomavirus detection in Merkel cell cancer tumors in Northern Germany using PCR and protein expression
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Merkel cell polyomavirus detection in Merkel cell cancer tumors in Northern Germany using PCR and protein expression. / Leitz, Miriam; Stieler, Kristin; Grundhoff, Adam; Moll, Ingrid; Brandner, Johanna M; Fischer, Nicole.
In: J MED VIROL, Vol. 86 , No. 10, 2014, p. 1813-1819.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Merkel cell polyomavirus detection in Merkel cell cancer tumors in Northern Germany using PCR and protein expression
AU - Leitz, Miriam
AU - Stieler, Kristin
AU - Grundhoff, Adam
AU - Moll, Ingrid
AU - Brandner, Johanna M
AU - Fischer, Nicole
N1 - © 2013 Wiley Periodicals, Inc.
PY - 2014
Y1 - 2014
N2 - Merkel cell carcinoma is a highly malignant skin cancer which predominantly occurs in elderly and immunocompromised persons. The identification of the Merkel cell polyomavirus (MCPyV) has inaugurated a new understanding of Merkel cell carcinoma pathogenesis. The frequent detection of the virus in Merkel cell carcinoma tissue (70-90%), its monoclonal integration in the tumor cells and the expression of viral oncogenes highly suggest that MCPyV is causally linked to the pathogenesis of the majority of Merkel cell cancer (MCC) cases. Using qualitative and quantitative PCR together with immunohistochemical staining this study aimed at characterizing the presence of MCPyV sequences and viral early gene expression in a cohort of MCC cases (n = 32) selected in Northern Germany. 40-57% of the cases were identified as MCPyV positive with 40.6% of the cases positive by immunohistochemical staining and 51.6-57.6% positive by PCR. Interestingly, in the majority (64%) of LT-Antigen positive tumors only 25-50% of tumor cells express LT-Antigen. These data are in accord with published studies describing heterogeneity in MCPyV viral loads and suggest that detection of MCPyV in Merkel cell carcinoma by PCR should be undertaken using multiple primer pairs. J. Med. Virol. © 2013 Wiley Periodicals, Inc.
AB - Merkel cell carcinoma is a highly malignant skin cancer which predominantly occurs in elderly and immunocompromised persons. The identification of the Merkel cell polyomavirus (MCPyV) has inaugurated a new understanding of Merkel cell carcinoma pathogenesis. The frequent detection of the virus in Merkel cell carcinoma tissue (70-90%), its monoclonal integration in the tumor cells and the expression of viral oncogenes highly suggest that MCPyV is causally linked to the pathogenesis of the majority of Merkel cell cancer (MCC) cases. Using qualitative and quantitative PCR together with immunohistochemical staining this study aimed at characterizing the presence of MCPyV sequences and viral early gene expression in a cohort of MCC cases (n = 32) selected in Northern Germany. 40-57% of the cases were identified as MCPyV positive with 40.6% of the cases positive by immunohistochemical staining and 51.6-57.6% positive by PCR. Interestingly, in the majority (64%) of LT-Antigen positive tumors only 25-50% of tumor cells express LT-Antigen. These data are in accord with published studies describing heterogeneity in MCPyV viral loads and suggest that detection of MCPyV in Merkel cell carcinoma by PCR should be undertaken using multiple primer pairs. J. Med. Virol. © 2013 Wiley Periodicals, Inc.
U2 - 10.1002/jmv.23808
DO - 10.1002/jmv.23808
M3 - SCORING: Journal article
C2 - 24307009
VL - 86
SP - 1813
EP - 1819
JO - J MED VIROL
JF - J MED VIROL
SN - 0146-6615
IS - 10
ER -
