Measuring Ca2+ release evoked by cyclic adp-ribose

Andreas H Guse

doi:10.1101/pdb.prot073015

Measuring Ca2+ release evoked by cyclic adp-ribose

Standard

Measuring Ca2+ release evoked by cyclic adp-ribose. / Guse, Andreas H.

In: Cold Spring Harbor protocols, Vol. 2013, No. 6, 01.06.2013, p. 574-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Guse, AH 2013, 'Measuring Ca2+ release evoked by cyclic adp-ribose', Cold Spring Harbor protocols, vol. 2013, no. 6, pp. 574-8. https://doi.org/10.1101/pdb.prot073015

APA

Guse, A. H. (2013). Measuring Ca2+ release evoked by cyclic adp-ribose. Cold Spring Harbor protocols, 2013(6), 574-8. https://doi.org/10.1101/pdb.prot073015

Vancouver

Guse AH. Measuring Ca2+ release evoked by cyclic adp-ribose. Cold Spring Harbor protocols. 2013 Jun 1;2013(6):574-8. https://doi.org/10.1101/pdb.prot073015

Bibtex

@article{f08f394bd2fa44df99462d2a06cd45e3,

title = "Measuring Ca2+ release evoked by cyclic adp-ribose",

abstract = "As a ubiquitous second messenger, the Ca(2+) mobilizing activity of cyclic ADP-ribose (cADPR) has been observed in many different cell types. The measurement of Ca(2+) release evoked by cADPR comprises several practical challenges. At physiological pH, cADPR has a net negative charge and it therefore cannot cross the cell membrane in cells that lack a suitable cADPR-transporting system. Thus, either the plasma membrane must be permeabilized or microinjection must be used to deliver cADPR to the cytosol. In this article, two methods for cADPR delivery (using permeabilized cells or microinjection) are explained step-by-step. Because most of our work has been performed using the Jurkat T-lymphoma cell line, the methods are tailored for this specific cell type. For other cell types, the procedures may need to be adapted.",

keywords = "Calcium, Cations, Divalent, Cell Membrane Permeability, Cyclic ADP-Ribose, Cytological Techniques, Humans, Jurkat Cells, Microinjections",

author = "Guse, {Andreas H}",

year = "2013",

month = jun,

day = "1",

doi = "10.1101/pdb.prot073015",

language = "English",

volume = "2013",

pages = "574--8",

journal = "Cold Spring Harbor protocols",

issn = "1559-6095",

publisher = "Cold Spring Harbor Laboratory Press",

number = "6",

}

RIS

TY - JOUR

T1 - Measuring Ca2+ release evoked by cyclic adp-ribose

AU - Guse, Andreas H

PY - 2013/6/1

Y1 - 2013/6/1

N2 - As a ubiquitous second messenger, the Ca(2+) mobilizing activity of cyclic ADP-ribose (cADPR) has been observed in many different cell types. The measurement of Ca(2+) release evoked by cADPR comprises several practical challenges. At physiological pH, cADPR has a net negative charge and it therefore cannot cross the cell membrane in cells that lack a suitable cADPR-transporting system. Thus, either the plasma membrane must be permeabilized or microinjection must be used to deliver cADPR to the cytosol. In this article, two methods for cADPR delivery (using permeabilized cells or microinjection) are explained step-by-step. Because most of our work has been performed using the Jurkat T-lymphoma cell line, the methods are tailored for this specific cell type. For other cell types, the procedures may need to be adapted.

AB - As a ubiquitous second messenger, the Ca(2+) mobilizing activity of cyclic ADP-ribose (cADPR) has been observed in many different cell types. The measurement of Ca(2+) release evoked by cADPR comprises several practical challenges. At physiological pH, cADPR has a net negative charge and it therefore cannot cross the cell membrane in cells that lack a suitable cADPR-transporting system. Thus, either the plasma membrane must be permeabilized or microinjection must be used to deliver cADPR to the cytosol. In this article, two methods for cADPR delivery (using permeabilized cells or microinjection) are explained step-by-step. Because most of our work has been performed using the Jurkat T-lymphoma cell line, the methods are tailored for this specific cell type. For other cell types, the procedures may need to be adapted.

KW - Calcium

KW - Cations, Divalent

KW - Cell Membrane Permeability

KW - Cyclic ADP-Ribose

KW - Cytological Techniques

KW - Humans

KW - Jurkat Cells

KW - Microinjections

U2 - 10.1101/pdb.prot073015

DO - 10.1101/pdb.prot073015

M3 - SCORING: Journal article

C2 - 23734018

VL - 2013

SP - 574

EP - 578

JO - Cold Spring Harbor protocols

JF - Cold Spring Harbor protocols

SN - 1559-6095

IS - 6

ER -