Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells
Standard
Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells. / Holzwarth, Christina; Vaegler, Martin; Gieseke, Friederike; Pfister, Stefan M; Handgretinger, Rupert; Kerst, Gunter; Müller, Ingo.
In: BMC CELL BIOL, Vol. 11, 2010, p. 11.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells
AU - Holzwarth, Christina
AU - Vaegler, Martin
AU - Gieseke, Friederike
AU - Pfister, Stefan M
AU - Handgretinger, Rupert
AU - Kerst, Gunter
AU - Müller, Ingo
PY - 2010
Y1 - 2010
N2 - BACKGROUND: Human multipotent mesenchymal stromal cells (MSC) can be isolated from various tissues including bone marrow. Here, MSC participate as bone lining cells in the formation of the hematopoietic stem cell niche. In this compartment, the oxygen tension is low and oxygen partial pressure is estimated to range from 1% to 7%. We analyzed the effect of low oxygen tensions on human MSC cultured with platelet-lysate supplemented media and assessed proliferation, morphology, chromosomal stability, immunophenotype and plasticity.RESULTS: After transferring MSC from atmospheric oxygen levels of 21% to 1%, HIF-1alpha expression was induced, indicating efficient oxygen reduction. Simultaneously, MSC exhibited a significantly different morphology with shorter extensions and broader cell bodies. MSC did not proliferate as rapidly as under 21% oxygen and accumulated in G1 phase. The immunophenotype, however, was unaffected. Hypoxic stress as well as free oxygen radicals may affect chromosomal stability. However, no chromosomal abnormalities in human MSC under either culture condition were detected using high-resolution matrix-based comparative genomic hybridization. Reduced oxygen tension severely impaired adipogenic and osteogenic differentiation of human MSC. Elevation of oxygen from 1% to 3% restored osteogenic differentiation.CONCLUSION: Physiologic oxygen tension during in vitro culture of human MSC slows down cell cycle progression and differentiation. Under physiological conditions this may keep a proportion of MSC in a resting state. Further studies are needed to analyze these aspects of MSC in tissue regeneration.
AB - BACKGROUND: Human multipotent mesenchymal stromal cells (MSC) can be isolated from various tissues including bone marrow. Here, MSC participate as bone lining cells in the formation of the hematopoietic stem cell niche. In this compartment, the oxygen tension is low and oxygen partial pressure is estimated to range from 1% to 7%. We analyzed the effect of low oxygen tensions on human MSC cultured with platelet-lysate supplemented media and assessed proliferation, morphology, chromosomal stability, immunophenotype and plasticity.RESULTS: After transferring MSC from atmospheric oxygen levels of 21% to 1%, HIF-1alpha expression was induced, indicating efficient oxygen reduction. Simultaneously, MSC exhibited a significantly different morphology with shorter extensions and broader cell bodies. MSC did not proliferate as rapidly as under 21% oxygen and accumulated in G1 phase. The immunophenotype, however, was unaffected. Hypoxic stress as well as free oxygen radicals may affect chromosomal stability. However, no chromosomal abnormalities in human MSC under either culture condition were detected using high-resolution matrix-based comparative genomic hybridization. Reduced oxygen tension severely impaired adipogenic and osteogenic differentiation of human MSC. Elevation of oxygen from 1% to 3% restored osteogenic differentiation.CONCLUSION: Physiologic oxygen tension during in vitro culture of human MSC slows down cell cycle progression and differentiation. Under physiological conditions this may keep a proportion of MSC in a resting state. Further studies are needed to analyze these aspects of MSC in tissue regeneration.
KW - Adolescent
KW - Bone Marrow Cells
KW - Cell Differentiation
KW - Cell Hypoxia
KW - Cell Proliferation
KW - Child
KW - Child, Preschool
KW - Chromosomal Instability
KW - Female
KW - G1 Phase
KW - Humans
KW - Hypoxia-Inducible Factor 1, alpha Subunit
KW - Immunophenotyping
KW - Infant
KW - Male
KW - Mesenchymal Stromal Cells
KW - Multipotent Stem Cells
KW - Stromal Cells
U2 - 10.1186/1471-2121-11-11
DO - 10.1186/1471-2121-11-11
M3 - SCORING: Journal article
C2 - 20109207
VL - 11
SP - 11
JO - BMC CELL BIOL
JF - BMC CELL BIOL
SN - 1471-2121
ER -
