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Loss of TP53 expression in immortalized choroid plexus epithelial cells results in increased resistance to anticancer agents

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Loss of TP53 expression in immortalized choroid plexus epithelial cells results in increased resistance to anticancer agents. / Krzyzankova, Miroslava; Mertsch, Sonja; Koos, Björn; Jeibmann, Astrid; Kruse, Anne; Kordes, Uwe; Frühwald, Michael C; Wolff, Johannes E; Paulus, Werner; Hasselblatt, Martin.

In: J NEURO-ONCOL, Vol. 109, No. 3, 01.09.2012, p. 449-55.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Krzyzankova, M, Mertsch, S, Koos, B, Jeibmann, A, Kruse, A, Kordes, U, Frühwald, MC, Wolff, JE, Paulus, W & Hasselblatt, M 2012, 'Loss of TP53 expression in immortalized choroid plexus epithelial cells results in increased resistance to anticancer agents', J NEURO-ONCOL, vol. 109, no. 3, pp. 449-55. https://doi.org/10.1007/s11060-012-0915-3

APA

Krzyzankova, M., Mertsch, S., Koos, B., Jeibmann, A., Kruse, A., Kordes, U., Frühwald, M. C., Wolff, J. E., Paulus, W., & Hasselblatt, M. (2012). Loss of TP53 expression in immortalized choroid plexus epithelial cells results in increased resistance to anticancer agents. J NEURO-ONCOL, 109(3), 449-55. https://doi.org/10.1007/s11060-012-0915-3

Vancouver

Krzyzankova M, Mertsch S, Koos B, Jeibmann A, Kruse A, Kordes U et al. Loss of TP53 expression in immortalized choroid plexus epithelial cells results in increased resistance to anticancer agents. J NEURO-ONCOL. 2012 Sep 1;109(3):449-55. https://doi.org/10.1007/s11060-012-0915-3

Bibtex

@article{1fae477f7d7f4b7b92074eded0a5082a,
title = "Loss of TP53 expression in immortalized choroid plexus epithelial cells results in increased resistance to anticancer agents",
abstract = "Choroid plexus carcinomas are malignant brain tumors predominantly arising in young children. Because a prognostic role of p53 alterations has been demonstrated, further research into potential underlying mechanisms is essential. Our objective was, therefore, to investigate the role of p53 in the growth-inhibitory potential of a variety of anticancer agents in the rodent choroid plexus epithelial cell line Z310. Furthermore, association of p53 alterations with proliferative activity (Ki67/MIB1) in choroid plexus carcinoma samples (N = 20) was examined by use of immunohistochemistry. Silencing of TP53 expression did not significantly alter metabolic activity in Z310 cells and p53 protein expression status was not associated with increased proliferative activity in choroid plexus carcinomas. However, the growth-inhibitory activity of vincristine, doxorubicin, carboplatin, etoposide, and temozolomide was significantly impaired by silencing of TP53. In conclusion, these results indicate a potential predictive role of p53 in choroid plexus carcinomas. Alterations of p53 should be taken into account when evaluating the effect of anticancer agents in future clinical trials.",
keywords = "Adolescent, Animals, Antineoplastic Agents, Carcinoma, Cell Line, Tumor, Cell Proliferation, Child, Child, Preschool, Choroid Plexus, Choroid Plexus Neoplasms, Drug Resistance, Neoplasm, Epithelial Cells, Female, Gene Expression Regulation, Neoplastic, Gene Silencing, Humans, Immunohistochemistry, Infant, Infant, Newborn, Male, Rats, Reverse Transcriptase Polymerase Chain Reaction, Tumor Suppressor Protein p53",
author = "Miroslava Krzyzankova and Sonja Mertsch and Bj{\"o}rn Koos and Astrid Jeibmann and Anne Kruse and Uwe Kordes and Fr{\"u}hwald, {Michael C} and Wolff, {Johannes E} and Werner Paulus and Martin Hasselblatt",
year = "2012",
month = sep,
day = "1",
doi = "10.1007/s11060-012-0915-3",
language = "English",
volume = "109",
pages = "449--55",
journal = "J NEURO-ONCOL",
issn = "0167-594X",
publisher = "Kluwer Academic Publishers",
number = "3",

}

RIS

TY - JOUR

T1 - Loss of TP53 expression in immortalized choroid plexus epithelial cells results in increased resistance to anticancer agents

AU - Krzyzankova, Miroslava

AU - Mertsch, Sonja

AU - Koos, Björn

AU - Jeibmann, Astrid

AU - Kruse, Anne

AU - Kordes, Uwe

AU - Frühwald, Michael C

AU - Wolff, Johannes E

AU - Paulus, Werner

AU - Hasselblatt, Martin

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Choroid plexus carcinomas are malignant brain tumors predominantly arising in young children. Because a prognostic role of p53 alterations has been demonstrated, further research into potential underlying mechanisms is essential. Our objective was, therefore, to investigate the role of p53 in the growth-inhibitory potential of a variety of anticancer agents in the rodent choroid plexus epithelial cell line Z310. Furthermore, association of p53 alterations with proliferative activity (Ki67/MIB1) in choroid plexus carcinoma samples (N = 20) was examined by use of immunohistochemistry. Silencing of TP53 expression did not significantly alter metabolic activity in Z310 cells and p53 protein expression status was not associated with increased proliferative activity in choroid plexus carcinomas. However, the growth-inhibitory activity of vincristine, doxorubicin, carboplatin, etoposide, and temozolomide was significantly impaired by silencing of TP53. In conclusion, these results indicate a potential predictive role of p53 in choroid plexus carcinomas. Alterations of p53 should be taken into account when evaluating the effect of anticancer agents in future clinical trials.

AB - Choroid plexus carcinomas are malignant brain tumors predominantly arising in young children. Because a prognostic role of p53 alterations has been demonstrated, further research into potential underlying mechanisms is essential. Our objective was, therefore, to investigate the role of p53 in the growth-inhibitory potential of a variety of anticancer agents in the rodent choroid plexus epithelial cell line Z310. Furthermore, association of p53 alterations with proliferative activity (Ki67/MIB1) in choroid plexus carcinoma samples (N = 20) was examined by use of immunohistochemistry. Silencing of TP53 expression did not significantly alter metabolic activity in Z310 cells and p53 protein expression status was not associated with increased proliferative activity in choroid plexus carcinomas. However, the growth-inhibitory activity of vincristine, doxorubicin, carboplatin, etoposide, and temozolomide was significantly impaired by silencing of TP53. In conclusion, these results indicate a potential predictive role of p53 in choroid plexus carcinomas. Alterations of p53 should be taken into account when evaluating the effect of anticancer agents in future clinical trials.

KW - Adolescent

KW - Animals

KW - Antineoplastic Agents

KW - Carcinoma

KW - Cell Line, Tumor

KW - Cell Proliferation

KW - Child

KW - Child, Preschool

KW - Choroid Plexus

KW - Choroid Plexus Neoplasms

KW - Drug Resistance, Neoplasm

KW - Epithelial Cells

KW - Female

KW - Gene Expression Regulation, Neoplastic

KW - Gene Silencing

KW - Humans

KW - Immunohistochemistry

KW - Infant

KW - Infant, Newborn

KW - Male

KW - Rats

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Tumor Suppressor Protein p53

U2 - 10.1007/s11060-012-0915-3

DO - 10.1007/s11060-012-0915-3

M3 - SCORING: Journal article

C2 - 22763759

VL - 109

SP - 449

EP - 455

JO - J NEURO-ONCOL

JF - J NEURO-ONCOL

SN - 0167-594X

IS - 3

ER -