Loss of retinal function in aged DBA/2J mice - New insights into retinal neurodegeneration
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Loss of retinal function in aged DBA/2J mice - New insights into retinal neurodegeneration. / Heiduschka, Peter; Julien, Sylvie; Schuettauf, Frank; Schnichels, Sven.
In: EXP EYE RES, Vol. 91, No. 5, 11.2010, p. 779-83.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Loss of retinal function in aged DBA/2J mice - New insights into retinal neurodegeneration
AU - Heiduschka, Peter
AU - Julien, Sylvie
AU - Schuettauf, Frank
AU - Schnichels, Sven
N1 - Copyright © 2010 Elsevier Ltd. All rights reserved.
PY - 2010/11
Y1 - 2010/11
N2 - The DBA/2J mouse is a common animal model of glaucoma. The intraocular pressure increases with age, and retinal ganglion cells (RGC) degenerate, usually starting at an age of approximately six months. In this study, we used two-year-old DBA/2J mice presuming an end-point of RGC degeneration. We investigated visual function in these animals using electroretinography (ERG) and visual evoked potentials (VEP), and we checked the number of remaining RGC by retrograde staining. Almost no RGC were left in the retina, and VEP were hardly recordable. Surprisingly, also ERG amplitudes of scotopic a-waves and b-waves, photopic b-waves and oscillatory potentials were decreased significantly by approximately 40% compared to amplitudes measured in age-matched C57BL/6J mice. The latencies were not changed in DBA/2J mice compared to C57BL/6J mice, and so were the ratios between amplitudes of a-waves, b-waves and oscillatory potentials. Our results indicate that, in addition to degeneration of RGC, also photoreceptors are affected by pathological processes in the eye caused by the mutations present in DBA/2J mice.
AB - The DBA/2J mouse is a common animal model of glaucoma. The intraocular pressure increases with age, and retinal ganglion cells (RGC) degenerate, usually starting at an age of approximately six months. In this study, we used two-year-old DBA/2J mice presuming an end-point of RGC degeneration. We investigated visual function in these animals using electroretinography (ERG) and visual evoked potentials (VEP), and we checked the number of remaining RGC by retrograde staining. Almost no RGC were left in the retina, and VEP were hardly recordable. Surprisingly, also ERG amplitudes of scotopic a-waves and b-waves, photopic b-waves and oscillatory potentials were decreased significantly by approximately 40% compared to amplitudes measured in age-matched C57BL/6J mice. The latencies were not changed in DBA/2J mice compared to C57BL/6J mice, and so were the ratios between amplitudes of a-waves, b-waves and oscillatory potentials. Our results indicate that, in addition to degeneration of RGC, also photoreceptors are affected by pathological processes in the eye caused by the mutations present in DBA/2J mice.
KW - Aging/physiology
KW - Animals
KW - Cell Count
KW - Disease Models, Animal
KW - Electroretinography
KW - Evoked Potentials, Visual/physiology
KW - Glaucoma/physiopathology
KW - Intraocular Pressure
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Inbred DBA
KW - Photoreceptor Cells, Vertebrate/pathology
KW - Retinal Degeneration/physiopathology
KW - Retinal Ganglion Cells/pathology
U2 - 10.1016/j.exer.2010.09.001
DO - 10.1016/j.exer.2010.09.001
M3 - SCORING: Journal article
C2 - 20832401
VL - 91
SP - 779
EP - 783
JO - EXP EYE RES
JF - EXP EYE RES
SN - 0014-4835
IS - 5
ER -
