Loss of protein expression and recurrent DNA hypermethylation of the GNG7 gene in squamous cell carcinoma of the head and neck.
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Loss of protein expression and recurrent DNA hypermethylation of the GNG7 gene in squamous cell carcinoma of the head and neck. / Hartmann, Sylvia; Szaumkessel, Marcin; Salaverria, Itziar; Simon, Ronald; Sauter, Guido; Kiwerska, Katarzyna; Gawecki, Wojciech; Bodnar, Magdalena; Marszalek, Andrzej; Richter, Julia; Brauze, Damian; Zemke, Natalia; Jarmuz, Malgorzata; Hansmann, Martin-Leo; Siebert, Reiner; Szyfter, Krzysztof; Giefing, Maciej.
In: J APPL GENET, Vol. 53, No. 2, 2, 2012, p. 167-174.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Loss of protein expression and recurrent DNA hypermethylation of the GNG7 gene in squamous cell carcinoma of the head and neck.
AU - Hartmann, Sylvia
AU - Szaumkessel, Marcin
AU - Salaverria, Itziar
AU - Simon, Ronald
AU - Sauter, Guido
AU - Kiwerska, Katarzyna
AU - Gawecki, Wojciech
AU - Bodnar, Magdalena
AU - Marszalek, Andrzej
AU - Richter, Julia
AU - Brauze, Damian
AU - Zemke, Natalia
AU - Jarmuz, Malgorzata
AU - Hansmann, Martin-Leo
AU - Siebert, Reiner
AU - Szyfter, Krzysztof
AU - Giefing, Maciej
PY - 2012
Y1 - 2012
N2 - Although down-regulation of GNG7 in cancer was reported before, its role in carcinogenesis is poorly understood. It belongs to a family of large G-proteins that may be involved in cell-contact-induced growth arrest and function in tumor suppression. In the present study, we stained immunohistochemically 188 tumors derived from larynx or floor of the mouth for GNG7 protein and confronted it with clinicopathologic data. Moreover, we performed bisulfite pyrosequencing to analyze GNG7 promoter methylation. We identified recurrent loss of GNG7 protein expression in 68/188 (36%) cases and promoter hypermethylation in (42/98; 43%) primary tumors, predominantly in young patients (p?<?0.001). Loss of GNG7 expression correlated with hypermethylation of GNG7 promoter region (p?<?0.001). Moreover, loss of GNG7 protein expression correlated with tumor size (p?=?0.012) and lack of cervical metastasis (p?=?0.02) whereas sustained expression correlated with keratinization (p?=?0.008). Taken together, loss of GNG7 protein expression is a frequent event in head and neck cancer. Moreover, our data suggest that hypermethylation of the promoter region of GNG7 is probably the mechanism of the observed inactivation.
AB - Although down-regulation of GNG7 in cancer was reported before, its role in carcinogenesis is poorly understood. It belongs to a family of large G-proteins that may be involved in cell-contact-induced growth arrest and function in tumor suppression. In the present study, we stained immunohistochemically 188 tumors derived from larynx or floor of the mouth for GNG7 protein and confronted it with clinicopathologic data. Moreover, we performed bisulfite pyrosequencing to analyze GNG7 promoter methylation. We identified recurrent loss of GNG7 protein expression in 68/188 (36%) cases and promoter hypermethylation in (42/98; 43%) primary tumors, predominantly in young patients (p?<?0.001). Loss of GNG7 expression correlated with hypermethylation of GNG7 promoter region (p?<?0.001). Moreover, loss of GNG7 protein expression correlated with tumor size (p?=?0.012) and lack of cervical metastasis (p?=?0.02) whereas sustained expression correlated with keratinization (p?=?0.008). Taken together, loss of GNG7 protein expression is a frequent event in head and neck cancer. Moreover, our data suggest that hypermethylation of the promoter region of GNG7 is probably the mechanism of the observed inactivation.
M3 - SCORING: Journal article
VL - 53
SP - 167
EP - 174
JO - J APPL GENET
JF - J APPL GENET
SN - 1234-1983
IS - 2
M1 - 2
ER -
