Loss of podocyte aPKClambda/iota causes polarity defects and nephrotic syndrome
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Loss of podocyte aPKClambda/iota causes polarity defects and nephrotic syndrome. / Huber, Tobias B; Hartleben, Björn; Winkelmann, Kirstin; Schneider, Lisa; Becker, Jan U; Leitges, Michael; Walz, Gerd; Haller, Hermann; Schiffer, Mario.
In: J AM SOC NEPHROL, Vol. 20, No. 4, 04.2009, p. 798-806.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Loss of podocyte aPKClambda/iota causes polarity defects and nephrotic syndrome
AU - Huber, Tobias B
AU - Hartleben, Björn
AU - Winkelmann, Kirstin
AU - Schneider, Lisa
AU - Becker, Jan U
AU - Leitges, Michael
AU - Walz, Gerd
AU - Haller, Hermann
AU - Schiffer, Mario
PY - 2009/4
Y1 - 2009/4
N2 - Atypical protein kinase C (aPKC) is a central component of the evolutionarily conserved Par3-Par6-aPKC complex, one of the fundamental regulators of cell polarity. We recently demonstrated that these proteins interact with Neph-nephrin molecules at the slit diaphragm of the glomerular filtration barrier. Here, we report that podocyte-specific deletion of aPKClambda/iota in mice results in severe proteinuria, nephrotic syndrome, and death at 4 to 5 wk after birth. Podocyte foot processes of knockout mice developed structural defects, including mislocalization of the slit diaphragm. In the glomerulus, aPKClambda/iota was primarily expressed in developing glomerular epithelial cells and podocyte foot processes. Interestingly, under physiologic conditions, aPKClambda/iota translocated from the apical surface to the basolateral side of developing podocytes, and this translocation preceded the development of foot processes and formation of slit diaphragms. Supporting a critical role for aPKClambda/iota in the maintenance of slit diaphragms and podocyte foot processes, aPKClambda/iota associated with the Neph-nephrin slit diaphragm complex and localized to the tips of filopodia and leading edges of cultured podocytes. These results suggest that aPKC signaling is fundamental to glomerular maintenance and development.
AB - Atypical protein kinase C (aPKC) is a central component of the evolutionarily conserved Par3-Par6-aPKC complex, one of the fundamental regulators of cell polarity. We recently demonstrated that these proteins interact with Neph-nephrin molecules at the slit diaphragm of the glomerular filtration barrier. Here, we report that podocyte-specific deletion of aPKClambda/iota in mice results in severe proteinuria, nephrotic syndrome, and death at 4 to 5 wk after birth. Podocyte foot processes of knockout mice developed structural defects, including mislocalization of the slit diaphragm. In the glomerulus, aPKClambda/iota was primarily expressed in developing glomerular epithelial cells and podocyte foot processes. Interestingly, under physiologic conditions, aPKClambda/iota translocated from the apical surface to the basolateral side of developing podocytes, and this translocation preceded the development of foot processes and formation of slit diaphragms. Supporting a critical role for aPKClambda/iota in the maintenance of slit diaphragms and podocyte foot processes, aPKClambda/iota associated with the Neph-nephrin slit diaphragm complex and localized to the tips of filopodia and leading edges of cultured podocytes. These results suggest that aPKC signaling is fundamental to glomerular maintenance and development.
KW - Animals
KW - Cell Polarity
KW - Death
KW - Gene Deletion
KW - Isoenzymes
KW - Kidney Glomerulus
KW - Mice
KW - Mice, Knockout
KW - Nephrotic Syndrome
KW - Podocytes
KW - Protein Kinase C
KW - Proteinuria
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1681/ASN.2008080871
DO - 10.1681/ASN.2008080871
M3 - SCORING: Journal article
C2 - 19279126
VL - 20
SP - 798
EP - 806
JO - J AM SOC NEPHROL
JF - J AM SOC NEPHROL
SN - 1046-6673
IS - 4
ER -