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Long time persistence and evolution of carbapenemase-producing Enterobacterales in the wastewater of a tertiary care hospital in Germany

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Long time persistence and evolution of carbapenemase-producing Enterobacterales in the wastewater of a tertiary care hospital in Germany. / Carlsen, Laura; Büttner, Henning; Christner, Martin; Cordts, Lukas; Franke, Gefion; Hoffmann, Armin; Knobling, Birte; Lütgehetmann, Marc; Nakel, Jacqueline; Werner, Thomas; Knobloch, Johannes K.

In: J INFECT PUBLIC HEAL, Vol. 16, No. 8, 08.2023, p. 1142-1148.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Carlsen, L, Büttner, H, Christner, M, Cordts, L, Franke, G, Hoffmann, A, Knobling, B, Lütgehetmann, M, Nakel, J, Werner, T & Knobloch, JK 2023, 'Long time persistence and evolution of carbapenemase-producing Enterobacterales in the wastewater of a tertiary care hospital in Germany', J INFECT PUBLIC HEAL, vol. 16, no. 8, pp. 1142-1148. https://doi.org/10.1016/j.jiph.2023.05.029

APA

Carlsen, L., Büttner, H., Christner, M., Cordts, L., Franke, G., Hoffmann, A., Knobling, B., Lütgehetmann, M., Nakel, J., Werner, T., & Knobloch, J. K. (2023). Long time persistence and evolution of carbapenemase-producing Enterobacterales in the wastewater of a tertiary care hospital in Germany. J INFECT PUBLIC HEAL, 16(8), 1142-1148. https://doi.org/10.1016/j.jiph.2023.05.029

Vancouver

Carlsen L, Büttner H, Christner M, Cordts L, Franke G, Hoffmann A et al. Long time persistence and evolution of carbapenemase-producing Enterobacterales in the wastewater of a tertiary care hospital in Germany. J INFECT PUBLIC HEAL. 2023 Aug;16(8):1142-1148. https://doi.org/10.1016/j.jiph.2023.05.029

Bibtex

@article{839f27d2111b410ca90a440dcd8a9460,
title = "Long time persistence and evolution of carbapenemase-producing Enterobacterales in the wastewater of a tertiary care hospital in Germany",
abstract = "BackgroundWorldwide observations revealed increased frequencies of multi-resistant Enterobacterales and resistance genes in hospital wastewater compared to any other type of wastewater. Despite the description of clonal lineages possibly adapted to hospital wastewater, little is known about long term persistence as well as evolution of these lineages.MethodsIn this study, wastewater isolates of different Enterobacterales species from a tertiary care hospital were investigated with 2.5 years distance. Whole Genome Sequencing (WGS) and resistance gene identification were performed for E. coli, C. freundii, S. marcescens, K. pneumoniae, K. oxytoca, and E. cloacae isolates (n = 59), isolated in 2022 and compared with strains isolated from the same wastewater pipeline in 2019 (n = 240).ResultsIndividual clonal lineages with highly related isolates could be identified in all species identified more than once in 2022 that appear to persist in the wastewater drainage. A common motif of all persistent clonal lineages was the carriage of mobile genetic elements encoding carbapenemase genes with hints for horizontal gene transfer in persistent clones in this environment observed over the 2.5-year period. Multiple plasmid replicons could be detected in both years. In 2022 isolates blaVIM-1 replaced blaOXA-48 as the most common carbapenemase gene compared to 2019. Interestingly, despite a similar abundance of carbapenemase genes (>80% of all isolates) at both time points genes encoding extended spectrum β-lactamases decreased over time.ConclusionsThis data indicates that hospital wastewater continuously releases genes encoding carbapenemases to the urban wastewater system. The evolution of the resident clones as well as the reasons for the selection advantage in this specific ecological niche needs to be further investigated in the future.",
author = "Laura Carlsen and Henning B{\"u}ttner and Martin Christner and Lukas Cordts and Gefion Franke and Armin Hoffmann and Birte Knobling and Marc L{\"u}tgehetmann and Jacqueline Nakel and Thomas Werner and Knobloch, {Johannes K.}",
year = "2023",
month = aug,
doi = "10.1016/j.jiph.2023.05.029",
language = "English",
volume = "16",
pages = "1142--1148",
journal = "J INFECT PUBLIC HEAL",
issn = "1876-0341",
publisher = "Elsevier BV",
number = "8",

}

RIS

TY - JOUR

T1 - Long time persistence and evolution of carbapenemase-producing Enterobacterales in the wastewater of a tertiary care hospital in Germany

AU - Carlsen, Laura

AU - Büttner, Henning

AU - Christner, Martin

AU - Cordts, Lukas

AU - Franke, Gefion

AU - Hoffmann, Armin

AU - Knobling, Birte

AU - Lütgehetmann, Marc

AU - Nakel, Jacqueline

AU - Werner, Thomas

AU - Knobloch, Johannes K.

PY - 2023/8

Y1 - 2023/8

N2 - BackgroundWorldwide observations revealed increased frequencies of multi-resistant Enterobacterales and resistance genes in hospital wastewater compared to any other type of wastewater. Despite the description of clonal lineages possibly adapted to hospital wastewater, little is known about long term persistence as well as evolution of these lineages.MethodsIn this study, wastewater isolates of different Enterobacterales species from a tertiary care hospital were investigated with 2.5 years distance. Whole Genome Sequencing (WGS) and resistance gene identification were performed for E. coli, C. freundii, S. marcescens, K. pneumoniae, K. oxytoca, and E. cloacae isolates (n = 59), isolated in 2022 and compared with strains isolated from the same wastewater pipeline in 2019 (n = 240).ResultsIndividual clonal lineages with highly related isolates could be identified in all species identified more than once in 2022 that appear to persist in the wastewater drainage. A common motif of all persistent clonal lineages was the carriage of mobile genetic elements encoding carbapenemase genes with hints for horizontal gene transfer in persistent clones in this environment observed over the 2.5-year period. Multiple plasmid replicons could be detected in both years. In 2022 isolates blaVIM-1 replaced blaOXA-48 as the most common carbapenemase gene compared to 2019. Interestingly, despite a similar abundance of carbapenemase genes (>80% of all isolates) at both time points genes encoding extended spectrum β-lactamases decreased over time.ConclusionsThis data indicates that hospital wastewater continuously releases genes encoding carbapenemases to the urban wastewater system. The evolution of the resident clones as well as the reasons for the selection advantage in this specific ecological niche needs to be further investigated in the future.

AB - BackgroundWorldwide observations revealed increased frequencies of multi-resistant Enterobacterales and resistance genes in hospital wastewater compared to any other type of wastewater. Despite the description of clonal lineages possibly adapted to hospital wastewater, little is known about long term persistence as well as evolution of these lineages.MethodsIn this study, wastewater isolates of different Enterobacterales species from a tertiary care hospital were investigated with 2.5 years distance. Whole Genome Sequencing (WGS) and resistance gene identification were performed for E. coli, C. freundii, S. marcescens, K. pneumoniae, K. oxytoca, and E. cloacae isolates (n = 59), isolated in 2022 and compared with strains isolated from the same wastewater pipeline in 2019 (n = 240).ResultsIndividual clonal lineages with highly related isolates could be identified in all species identified more than once in 2022 that appear to persist in the wastewater drainage. A common motif of all persistent clonal lineages was the carriage of mobile genetic elements encoding carbapenemase genes with hints for horizontal gene transfer in persistent clones in this environment observed over the 2.5-year period. Multiple plasmid replicons could be detected in both years. In 2022 isolates blaVIM-1 replaced blaOXA-48 as the most common carbapenemase gene compared to 2019. Interestingly, despite a similar abundance of carbapenemase genes (>80% of all isolates) at both time points genes encoding extended spectrum β-lactamases decreased over time.ConclusionsThis data indicates that hospital wastewater continuously releases genes encoding carbapenemases to the urban wastewater system. The evolution of the resident clones as well as the reasons for the selection advantage in this specific ecological niche needs to be further investigated in the future.

U2 - 10.1016/j.jiph.2023.05.029

DO - 10.1016/j.jiph.2023.05.029

M3 - SCORING: Journal article

C2 - 37267681

VL - 16

SP - 1142

EP - 1148

JO - J INFECT PUBLIC HEAL

JF - J INFECT PUBLIC HEAL

SN - 1876-0341

IS - 8

ER -