Liquid application method for time-resolved analyses by serial synchrotron crystallography
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Abstract
We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.
Bibliographical data
Original language | English |
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ISSN | 1548-7091 |
DOIs | |
Publication status | Published - 10.2019 |
PubMed | 31527838 |
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