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Lipidome Analysis of Oropharyngeal Tumor Tissues Using Nanosecond Infrared Laser (NIRL) Tissue Sampling and Subsequent Mass Spectrometry

Standard

Lipidome Analysis of Oropharyngeal Tumor Tissues Using Nanosecond Infrared Laser (NIRL) Tissue Sampling and Subsequent Mass Spectrometry. / Stadlhofer, Rupert; Moritz, Manuela; Fuh, Marceline Manka; Heeren, Jörg; Zech, Henrike Barbara Antonia Angelika; Clauditz, Till Sebastian; Schlüter, Hartmut; Betz, Christian Stephan; Eggert, Dennis; Böttcher, Arne; Hahn, Jan.

In: INT J MOL SCI, Vol. 24, No. 9, 7820, 25.04.2023.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Stadlhofer, R, Moritz, M, Fuh, MM, Heeren, J, Zech, HBAA, Clauditz, TS, Schlüter, H, Betz, CS, Eggert, D, Böttcher, A & Hahn, J 2023, 'Lipidome Analysis of Oropharyngeal Tumor Tissues Using Nanosecond Infrared Laser (NIRL) Tissue Sampling and Subsequent Mass Spectrometry', INT J MOL SCI, vol. 24, no. 9, 7820. https://doi.org/10.3390/ijms24097820

APA

Stadlhofer, R., Moritz, M., Fuh, M. M., Heeren, J., Zech, H. B. A. A., Clauditz, T. S., Schlüter, H., Betz, C. S., Eggert, D., Böttcher, A., & Hahn, J. (2023). Lipidome Analysis of Oropharyngeal Tumor Tissues Using Nanosecond Infrared Laser (NIRL) Tissue Sampling and Subsequent Mass Spectrometry. INT J MOL SCI, 24(9), [7820]. https://doi.org/10.3390/ijms24097820

Vancouver

Stadlhofer R, Moritz M, Fuh MM, Heeren J, Zech HBAA, Clauditz TS et al. Lipidome Analysis of Oropharyngeal Tumor Tissues Using Nanosecond Infrared Laser (NIRL) Tissue Sampling and Subsequent Mass Spectrometry. INT J MOL SCI. 2023 Apr 25;24(9). 7820. https://doi.org/10.3390/ijms24097820

Bibtex

@article{615a88bc974b4b98baab7cf213a8de6f,
title = "Lipidome Analysis of Oropharyngeal Tumor Tissues Using Nanosecond Infrared Laser (NIRL) Tissue Sampling and Subsequent Mass Spectrometry",
abstract = "Ultrashort pulse infrared lasers can simultaneously sample and homogenize biological tissue using desorption by impulsive vibrational excitation (DIVE). With growing attention on alterations in lipid metabolism in malignant disease, mass spectrometry (MS)-based lipidomic analysis has become an emerging topic in cancer research. In this pilot study, we investigated the feasibility of tissue sampling with a nanosecond infrared laser (NIRL) for the subsequent lipidomic analysis of oropharyngeal tissues, and its potential to discriminate oropharyngeal squamous cell carcinoma (OPSCC) from non-tumorous oropharyngeal tissue. Eleven fresh frozen oropharyngeal tissue samples were ablated. The produced aerosols were collected by a glass fiber filter, and the lipidomes were analyzed with mass spectrometry. Data was evaluated by principal component analysis and Welch's t-tests. Lipid profiles comprised 13 lipid classes and up to 755 lipid species. We found significant inter- and intrapatient alterations in lipid profiles for tumor and non-tumor samples (p-value < 0.05, two-fold difference). Thus, NIRL tissue sampling with consecutive MS lipidomic analysis is a feasible and promising approach for the differentiation of OPSCC and non-tumorous oropharyngeal tissue and may provide new insights into lipid composition alterations in OPSCC.",
author = "Rupert Stadlhofer and Manuela Moritz and Fuh, {Marceline Manka} and J{\"o}rg Heeren and Zech, {Henrike Barbara Antonia Angelika} and Clauditz, {Till Sebastian} and Hartmut Schl{\"u}ter and Betz, {Christian Stephan} and Dennis Eggert and Arne B{\"o}ttcher and Jan Hahn",
year = "2023",
month = apr,
day = "25",
doi = "10.3390/ijms24097820",
language = "English",
volume = "24",
journal = "INT J MOL SCI",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "9",

}

RIS

TY - JOUR

T1 - Lipidome Analysis of Oropharyngeal Tumor Tissues Using Nanosecond Infrared Laser (NIRL) Tissue Sampling and Subsequent Mass Spectrometry

AU - Stadlhofer, Rupert

AU - Moritz, Manuela

AU - Fuh, Marceline Manka

AU - Heeren, Jörg

AU - Zech, Henrike Barbara Antonia Angelika

AU - Clauditz, Till Sebastian

AU - Schlüter, Hartmut

AU - Betz, Christian Stephan

AU - Eggert, Dennis

AU - Böttcher, Arne

AU - Hahn, Jan

PY - 2023/4/25

Y1 - 2023/4/25

N2 - Ultrashort pulse infrared lasers can simultaneously sample and homogenize biological tissue using desorption by impulsive vibrational excitation (DIVE). With growing attention on alterations in lipid metabolism in malignant disease, mass spectrometry (MS)-based lipidomic analysis has become an emerging topic in cancer research. In this pilot study, we investigated the feasibility of tissue sampling with a nanosecond infrared laser (NIRL) for the subsequent lipidomic analysis of oropharyngeal tissues, and its potential to discriminate oropharyngeal squamous cell carcinoma (OPSCC) from non-tumorous oropharyngeal tissue. Eleven fresh frozen oropharyngeal tissue samples were ablated. The produced aerosols were collected by a glass fiber filter, and the lipidomes were analyzed with mass spectrometry. Data was evaluated by principal component analysis and Welch's t-tests. Lipid profiles comprised 13 lipid classes and up to 755 lipid species. We found significant inter- and intrapatient alterations in lipid profiles for tumor and non-tumor samples (p-value < 0.05, two-fold difference). Thus, NIRL tissue sampling with consecutive MS lipidomic analysis is a feasible and promising approach for the differentiation of OPSCC and non-tumorous oropharyngeal tissue and may provide new insights into lipid composition alterations in OPSCC.

AB - Ultrashort pulse infrared lasers can simultaneously sample and homogenize biological tissue using desorption by impulsive vibrational excitation (DIVE). With growing attention on alterations in lipid metabolism in malignant disease, mass spectrometry (MS)-based lipidomic analysis has become an emerging topic in cancer research. In this pilot study, we investigated the feasibility of tissue sampling with a nanosecond infrared laser (NIRL) for the subsequent lipidomic analysis of oropharyngeal tissues, and its potential to discriminate oropharyngeal squamous cell carcinoma (OPSCC) from non-tumorous oropharyngeal tissue. Eleven fresh frozen oropharyngeal tissue samples were ablated. The produced aerosols were collected by a glass fiber filter, and the lipidomes were analyzed with mass spectrometry. Data was evaluated by principal component analysis and Welch's t-tests. Lipid profiles comprised 13 lipid classes and up to 755 lipid species. We found significant inter- and intrapatient alterations in lipid profiles for tumor and non-tumor samples (p-value < 0.05, two-fold difference). Thus, NIRL tissue sampling with consecutive MS lipidomic analysis is a feasible and promising approach for the differentiation of OPSCC and non-tumorous oropharyngeal tissue and may provide new insights into lipid composition alterations in OPSCC.

U2 - 10.3390/ijms24097820

DO - 10.3390/ijms24097820

M3 - SCORING: Journal article

C2 - 37175533

VL - 24

JO - INT J MOL SCI

JF - INT J MOL SCI

SN - 1661-6596

IS - 9

M1 - 7820

ER -