Research ExplorerPublicationsLIN28A immunoreactivity is a potent diagnostic marker of emb...

LIN28A immunoreactivity is a potent diagnostic marker of embryonal tumor with multilayered rosettes (ETMR)

Standard

LIN28A immunoreactivity is a potent diagnostic marker of embryonal tumor with multilayered rosettes (ETMR). / Korshunov, Andrey; Ryzhova, Marina; Jones, David T W; Northcott, Paul A; van Sluis, Peter; Volckmann, Richard; Koster, Jan; Versteeg, Rogier; Cowdrey, Cynthia J; Perry, Arie; Picard, Daniel; Rosenblum, Marc; Giangaspero, Felice; Aronica, Eleonora; Schüller, Ulrich; Hasselblatt, Martin; Collins, V Peter; Deimling, Andreas; Lichter, Peter; Huang, Annie; Pfister, Stefan M; Kool, Marcel.

In: ACTA NEUROPATHOL, Vol. 124, No. 6, 12.2012, p. 875-81.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Korshunov, A, Ryzhova, M, Jones, DTW, Northcott, PA, van Sluis, P, Volckmann, R, Koster, J, Versteeg, R, Cowdrey, CJ, Perry, A, Picard, D, Rosenblum, M, Giangaspero, F, Aronica, E, Schüller, U, Hasselblatt, M, Collins, VP, Deimling, A, Lichter, P, Huang, A, Pfister, SM & Kool, M 2012, 'LIN28A immunoreactivity is a potent diagnostic marker of embryonal tumor with multilayered rosettes (ETMR)', ACTA NEUROPATHOL, vol. 124, no. 6, pp. 875-81. https://doi.org/10.1007/s00401-012-1068-3

APA

Korshunov, A., Ryzhova, M., Jones, D. T. W., Northcott, P. A., van Sluis, P., Volckmann, R., Koster, J., Versteeg, R., Cowdrey, C. J., Perry, A., Picard, D., Rosenblum, M., Giangaspero, F., Aronica, E., Schüller, U., Hasselblatt, M., Collins, V. P., Deimling, A., Lichter, P., ... Kool, M. (2012). LIN28A immunoreactivity is a potent diagnostic marker of embryonal tumor with multilayered rosettes (ETMR). ACTA NEUROPATHOL, 124(6), 875-81. https://doi.org/10.1007/s00401-012-1068-3

Vancouver

Korshunov A, Ryzhova M, Jones DTW, Northcott PA, van Sluis P, Volckmann R et al. LIN28A immunoreactivity is a potent diagnostic marker of embryonal tumor with multilayered rosettes (ETMR). ACTA NEUROPATHOL. 2012 Dec;124(6):875-81. https://doi.org/10.1007/s00401-012-1068-3

Bibtex

@article{9576330a685d4cd1a79bc60bd219a0e0,
title = "LIN28A immunoreactivity is a potent diagnostic marker of embryonal tumor with multilayered rosettes (ETMR)",
abstract = "Embryonal tumor with multilayered rosettes (ETMR, previously known as ETANTR) is a highly aggressive embryonal CNS tumor, which almost exclusively affects infants and is associated with a dismal prognosis. Accurate diagnosis is of critical clinical importance because of its poor response to current treatment protocols and its distinct biology. Amplification of the miRNA cluster at 19q13.42 has been identified previously as a genetic hallmark for ETMR, but an immunohistochemistry-based assay for clinical routine diagnostics [such as INI-1 for atypical teratoid rhabdoid tumor (AT/RT)] is still lacking. In this study, we screened for an ETMR-specific marker using a gene-expression profiling dataset of more than 1,400 brain tumors and identified LIN28A as a highly specific marker for ETMR. The encoded protein binds small RNA and has been implicated in stem cell pluripotency, metabolism and tumorigenesis. Using an LIN28A specific antibody, we carried out immunohistochemical analysis of LIN28A in more than 800 childhood brain-tumor samples and confirmed its high specificity for ETMR. Strong LIN28A immunoexpression was found in all 37 ETMR samples tested, whereas focal reactivity was only present in a small (6/50) proportion of AT/RT samples. All other pediatric brain tumors were completely LIN28A-negative. In summary, we established LIN28A immunohistochemistry as a highly sensitive and specific, rapid, inexpensive diagnostic tool for routine pathological verification of ETMR.",
keywords = "Adolescent, Biomarkers, Tumor, Brain Neoplasms, Child, Child, Preschool, DNA-Binding Proteins, Diagnosis, Differential, Gene Expression Profiling, Humans, MicroRNAs, Neoplasms, Germ Cell and Embryonal, Neuroectodermal Tumors, Primitive, Neuropil, RNA-Binding Proteins, Rhabdoid Tumor, Journal Article, Research Support, Non-U.S. Gov't",
author = "Andrey Korshunov and Marina Ryzhova and Jones, {David T W} and Northcott, {Paul A} and {van Sluis}, Peter and Richard Volckmann and Jan Koster and Rogier Versteeg and Cowdrey, {Cynthia J} and Arie Perry and Daniel Picard and Marc Rosenblum and Felice Giangaspero and Eleonora Aronica and Ulrich Sch{\"u}ller and Martin Hasselblatt and Collins, {V Peter} and Andreas Deimling and Peter Lichter and Annie Huang and Pfister, {Stefan M} and Marcel Kool",
year = "2012",
month = dec,
doi = "10.1007/s00401-012-1068-3",
language = "English",
volume = "124",
pages = "875--81",
journal = "ACTA NEUROPATHOL",
issn = "0001-6322",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - LIN28A immunoreactivity is a potent diagnostic marker of embryonal tumor with multilayered rosettes (ETMR)

AU - Korshunov, Andrey

AU - Ryzhova, Marina

AU - Jones, David T W

AU - Northcott, Paul A

AU - van Sluis, Peter

AU - Volckmann, Richard

AU - Koster, Jan

AU - Versteeg, Rogier

AU - Cowdrey, Cynthia J

AU - Perry, Arie

AU - Picard, Daniel

AU - Rosenblum, Marc

AU - Giangaspero, Felice

AU - Aronica, Eleonora

AU - Schüller, Ulrich

AU - Hasselblatt, Martin

AU - Collins, V Peter

AU - Deimling, Andreas

AU - Lichter, Peter

AU - Huang, Annie

AU - Pfister, Stefan M

AU - Kool, Marcel

PY - 2012/12

Y1 - 2012/12

N2 - Embryonal tumor with multilayered rosettes (ETMR, previously known as ETANTR) is a highly aggressive embryonal CNS tumor, which almost exclusively affects infants and is associated with a dismal prognosis. Accurate diagnosis is of critical clinical importance because of its poor response to current treatment protocols and its distinct biology. Amplification of the miRNA cluster at 19q13.42 has been identified previously as a genetic hallmark for ETMR, but an immunohistochemistry-based assay for clinical routine diagnostics [such as INI-1 for atypical teratoid rhabdoid tumor (AT/RT)] is still lacking. In this study, we screened for an ETMR-specific marker using a gene-expression profiling dataset of more than 1,400 brain tumors and identified LIN28A as a highly specific marker for ETMR. The encoded protein binds small RNA and has been implicated in stem cell pluripotency, metabolism and tumorigenesis. Using an LIN28A specific antibody, we carried out immunohistochemical analysis of LIN28A in more than 800 childhood brain-tumor samples and confirmed its high specificity for ETMR. Strong LIN28A immunoexpression was found in all 37 ETMR samples tested, whereas focal reactivity was only present in a small (6/50) proportion of AT/RT samples. All other pediatric brain tumors were completely LIN28A-negative. In summary, we established LIN28A immunohistochemistry as a highly sensitive and specific, rapid, inexpensive diagnostic tool for routine pathological verification of ETMR.

AB - Embryonal tumor with multilayered rosettes (ETMR, previously known as ETANTR) is a highly aggressive embryonal CNS tumor, which almost exclusively affects infants and is associated with a dismal prognosis. Accurate diagnosis is of critical clinical importance because of its poor response to current treatment protocols and its distinct biology. Amplification of the miRNA cluster at 19q13.42 has been identified previously as a genetic hallmark for ETMR, but an immunohistochemistry-based assay for clinical routine diagnostics [such as INI-1 for atypical teratoid rhabdoid tumor (AT/RT)] is still lacking. In this study, we screened for an ETMR-specific marker using a gene-expression profiling dataset of more than 1,400 brain tumors and identified LIN28A as a highly specific marker for ETMR. The encoded protein binds small RNA and has been implicated in stem cell pluripotency, metabolism and tumorigenesis. Using an LIN28A specific antibody, we carried out immunohistochemical analysis of LIN28A in more than 800 childhood brain-tumor samples and confirmed its high specificity for ETMR. Strong LIN28A immunoexpression was found in all 37 ETMR samples tested, whereas focal reactivity was only present in a small (6/50) proportion of AT/RT samples. All other pediatric brain tumors were completely LIN28A-negative. In summary, we established LIN28A immunohistochemistry as a highly sensitive and specific, rapid, inexpensive diagnostic tool for routine pathological verification of ETMR.

KW - Adolescent

KW - Biomarkers, Tumor

KW - Brain Neoplasms

KW - Child

KW - Child, Preschool

KW - DNA-Binding Proteins

KW - Diagnosis, Differential

KW - Gene Expression Profiling

KW - Humans

KW - MicroRNAs

KW - Neoplasms, Germ Cell and Embryonal

KW - Neuroectodermal Tumors, Primitive

KW - Neuropil

KW - RNA-Binding Proteins

KW - Rhabdoid Tumor

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s00401-012-1068-3

DO - 10.1007/s00401-012-1068-3

M3 - SCORING: Journal article

C2 - 23161096

VL - 124

SP - 875

EP - 881

JO - ACTA NEUROPATHOL

JF - ACTA NEUROPATHOL

SN - 0001-6322

IS - 6

ER -