Research ExplorerPublicationsLevels of vascular endothelial growth factor, hepatocyte gro...

Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis

Standard

Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis. / Schmidt, N O; Westphal, M; Hagel, C; Ergün, S; Stavrou, D; Rosen, E M; Lamszus, K.

In: INT J CANCER, Vol. 84, No. 1, 1, 19.02.1999, p. 10-18.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schmidt, NO, Westphal, M, Hagel, C, Ergün, S, Stavrou, D, Rosen, EM & Lamszus, K 1999, 'Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis', INT J CANCER, vol. 84, no. 1, 1, pp. 10-18. https://doi.org/10.1002/(sici)1097-0215(19990219)84:1<10::aid-ijc3>3.0.co;2-l

APA

Schmidt, N. O., Westphal, M., Hagel, C., Ergün, S., Stavrou, D., Rosen, E. M., & Lamszus, K. (1999). Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis. INT J CANCER, 84(1), 10-18. [1]. https://doi.org/10.1002/(sici)1097-0215(19990219)84:1<10::aid-ijc3>3.0.co;2-l

Vancouver

Schmidt NO, Westphal M, Hagel C, Ergün S, Stavrou D, Rosen EM et al. Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis. INT J CANCER. 1999 Feb 19;84(1):10-18. 1. https://doi.org/10.1002/(sici)1097-0215(19990219)84:1<10::aid-ijc3>3.0.co;2-l

Bibtex

@article{f1a3b0f36399490d9ad6ddaccd3194da,
title = "Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis",
abstract = "Angiogenesis is a possible target in the treatment of human gliomas. To evaluate the role of 3 growth factors, vascular endothelial growth factor (VEGF), hepatocyte growth factor/scatter factor (HGF/SF) and basic fibroblast growth factor (bFGF), in the angiogenic cascade, we determined their levels in extracts of 71 gliomas by enzyme-linked immunosorbent assay (ELISA). The levels of bFGF were only marginally different between gliomas of World Health Organization (WHO) grade II (low grade) and grades III and IV (high grade). In contrast, the mean concentrations of VEGF were 11-fold higher in high-grade tumors and those of HGF/SF 7-fold, respectively. Both were highly significantly correlated with microvessel density (p < 0.001) as determined by immunostaining for factor VIII-related antigen. In addition, VEGF and HGF/SF appeared to be independent predictive parameters for glioma microvessel density as determined by multiple regression analysis. We measured the capacity of all 3 factors to induce endothelial tube formation in a collagen gel. In this assay, bFGF was found to be an essential cofactor with which VEGF as well as HGF/SF were able to synergize independently. According to the concentrations of angiogenic factors, extracts from high-grade tumors were significantly more potent in the tube formation assay than the low-grade extracts (p = 0.02). Adding neutralizing antibodies to bFGF, VEGF and HGF/SF together with the extracts, tube formation was inhibited by up to 98%, 62% and 54%, respectively. Our findings suggest that bFGF is an essential cofactor for angiogenesis in gliomas, but in itself is insufficient as it is present already in the sparsely vascularized low-grade tumors. Upon induction of angiogenesis in high-grade tumors, bFGF may synergize with rising levels of not only VEGF but possibly also with HGF/SF, which appears here to be an independent angiogenic factor.",
keywords = "Cells, Cultured, Chemotaxis/drug effects, Endothelial Growth Factors/metabolism, Endothelium, Vascular/metabolism, Enzyme-Linked Immunosorbent Assay, Fibroblast Growth Factor 2/metabolism, Glioma/blood supply, Hepatocyte Growth Factor/metabolism, Humans, Lymphokines/metabolism, Microcirculation/drug effects, Neovascularization, Pathologic, Recombinant Proteins/metabolism, Up-Regulation, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors",
author = "Schmidt, {N O} and M Westphal and C Hagel and S Erg{\"u}n and D Stavrou and Rosen, {E M} and K Lamszus",
year = "1999",
month = feb,
day = "19",
doi = "10.1002/(sici)1097-0215(19990219)84:1<10::aid-ijc3>3.0.co;2-l",
language = "English",
volume = "84",
pages = "10--18",
journal = "INT J CANCER",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis

AU - Schmidt, N O

AU - Westphal, M

AU - Hagel, C

AU - Ergün, S

AU - Stavrou, D

AU - Rosen, E M

AU - Lamszus, K

PY - 1999/2/19

Y1 - 1999/2/19

N2 - Angiogenesis is a possible target in the treatment of human gliomas. To evaluate the role of 3 growth factors, vascular endothelial growth factor (VEGF), hepatocyte growth factor/scatter factor (HGF/SF) and basic fibroblast growth factor (bFGF), in the angiogenic cascade, we determined their levels in extracts of 71 gliomas by enzyme-linked immunosorbent assay (ELISA). The levels of bFGF were only marginally different between gliomas of World Health Organization (WHO) grade II (low grade) and grades III and IV (high grade). In contrast, the mean concentrations of VEGF were 11-fold higher in high-grade tumors and those of HGF/SF 7-fold, respectively. Both were highly significantly correlated with microvessel density (p < 0.001) as determined by immunostaining for factor VIII-related antigen. In addition, VEGF and HGF/SF appeared to be independent predictive parameters for glioma microvessel density as determined by multiple regression analysis. We measured the capacity of all 3 factors to induce endothelial tube formation in a collagen gel. In this assay, bFGF was found to be an essential cofactor with which VEGF as well as HGF/SF were able to synergize independently. According to the concentrations of angiogenic factors, extracts from high-grade tumors were significantly more potent in the tube formation assay than the low-grade extracts (p = 0.02). Adding neutralizing antibodies to bFGF, VEGF and HGF/SF together with the extracts, tube formation was inhibited by up to 98%, 62% and 54%, respectively. Our findings suggest that bFGF is an essential cofactor for angiogenesis in gliomas, but in itself is insufficient as it is present already in the sparsely vascularized low-grade tumors. Upon induction of angiogenesis in high-grade tumors, bFGF may synergize with rising levels of not only VEGF but possibly also with HGF/SF, which appears here to be an independent angiogenic factor.

AB - Angiogenesis is a possible target in the treatment of human gliomas. To evaluate the role of 3 growth factors, vascular endothelial growth factor (VEGF), hepatocyte growth factor/scatter factor (HGF/SF) and basic fibroblast growth factor (bFGF), in the angiogenic cascade, we determined their levels in extracts of 71 gliomas by enzyme-linked immunosorbent assay (ELISA). The levels of bFGF were only marginally different between gliomas of World Health Organization (WHO) grade II (low grade) and grades III and IV (high grade). In contrast, the mean concentrations of VEGF were 11-fold higher in high-grade tumors and those of HGF/SF 7-fold, respectively. Both were highly significantly correlated with microvessel density (p < 0.001) as determined by immunostaining for factor VIII-related antigen. In addition, VEGF and HGF/SF appeared to be independent predictive parameters for glioma microvessel density as determined by multiple regression analysis. We measured the capacity of all 3 factors to induce endothelial tube formation in a collagen gel. In this assay, bFGF was found to be an essential cofactor with which VEGF as well as HGF/SF were able to synergize independently. According to the concentrations of angiogenic factors, extracts from high-grade tumors were significantly more potent in the tube formation assay than the low-grade extracts (p = 0.02). Adding neutralizing antibodies to bFGF, VEGF and HGF/SF together with the extracts, tube formation was inhibited by up to 98%, 62% and 54%, respectively. Our findings suggest that bFGF is an essential cofactor for angiogenesis in gliomas, but in itself is insufficient as it is present already in the sparsely vascularized low-grade tumors. Upon induction of angiogenesis in high-grade tumors, bFGF may synergize with rising levels of not only VEGF but possibly also with HGF/SF, which appears here to be an independent angiogenic factor.

KW - Cells, Cultured

KW - Chemotaxis/drug effects

KW - Endothelial Growth Factors/metabolism

KW - Endothelium, Vascular/metabolism

KW - Enzyme-Linked Immunosorbent Assay

KW - Fibroblast Growth Factor 2/metabolism

KW - Glioma/blood supply

KW - Hepatocyte Growth Factor/metabolism

KW - Humans

KW - Lymphokines/metabolism

KW - Microcirculation/drug effects

KW - Neovascularization, Pathologic

KW - Recombinant Proteins/metabolism

KW - Up-Regulation

KW - Vascular Endothelial Growth Factor A

KW - Vascular Endothelial Growth Factors

U2 - 10.1002/(sici)1097-0215(19990219)84:1<10::aid-ijc3>3.0.co;2-l

DO - 10.1002/(sici)1097-0215(19990219)84:1<10::aid-ijc3>3.0.co;2-l

M3 - SCORING: Journal article

C2 - 9988225

VL - 84

SP - 10

EP - 18

JO - INT J CANCER

JF - INT J CANCER

SN - 0020-7136

IS - 1

M1 - 1

ER -