Lectin histochemistry of the spleen
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Lectin histochemistry of the spleen : a new lectin visualizes the stromal architecture of white pulp and the sinuses of red pulp. / Düllmann, J; Feldhaus, S; Van Damme, E J; Peumans, W J; Schumacher, U.
In: J HISTOCHEM CYTOCHEM, Vol. 48, No. 7, 07.2000, p. 923-31.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Lectin histochemistry of the spleen
T2 - a new lectin visualizes the stromal architecture of white pulp and the sinuses of red pulp
AU - Düllmann, J
AU - Feldhaus, S
AU - Van Damme, E J
AU - Peumans, W J
AU - Schumacher, U
PY - 2000/7
Y1 - 2000/7
N2 - The subcompartmentalization of the white pulp in the spleen is the result of interactions of specific resident stromal cells and migrating subtypes of lymphocytes. Because carbohydrate residues of cell membranes and extracellular matrices are involved in cell-cell and cell-matrix interactions, they were investigated in rat spleen by a broad panel of lectins. Splenic macrophages, which were also demonstrated by Perls' Prussian blue reaction, were labeled selectively by most mannose-specific lectins and gave the characteristic distribution patterns in all splenic (sub)compartments. One recently isolated lectin, Chelidonium majus agglutinin (CMA), visualized predominantly central arterioles, the reticular meshwork (RM) in the periarteriolar lymphatic sheaths (PALS), the circumferential reticulum cells limiting PALS and follicles, and some follicular dendritic cells (FDCs) in white pulp. The endothelial cells of venous sinuses in red pulp were also labeled by CMA and, if frozen sections were used, CMA also labeled the macrophages of the red pulp. Compared to CMA, the monoclonal antibody CD11, which can be used only in frozen sections, stained almost solely the fibrous (extracellular) component of the RM. Because CMA stains the reticulum cells in particular, it is better suited to visualize the stromal architecture of splenic white pulp than the monoclonal antibody. Because CMA can be applied to paraffin-embedded material, it is a particularly useful tool to study the splenic stromal architecture in archival material.
AB - The subcompartmentalization of the white pulp in the spleen is the result of interactions of specific resident stromal cells and migrating subtypes of lymphocytes. Because carbohydrate residues of cell membranes and extracellular matrices are involved in cell-cell and cell-matrix interactions, they were investigated in rat spleen by a broad panel of lectins. Splenic macrophages, which were also demonstrated by Perls' Prussian blue reaction, were labeled selectively by most mannose-specific lectins and gave the characteristic distribution patterns in all splenic (sub)compartments. One recently isolated lectin, Chelidonium majus agglutinin (CMA), visualized predominantly central arterioles, the reticular meshwork (RM) in the periarteriolar lymphatic sheaths (PALS), the circumferential reticulum cells limiting PALS and follicles, and some follicular dendritic cells (FDCs) in white pulp. The endothelial cells of venous sinuses in red pulp were also labeled by CMA and, if frozen sections were used, CMA also labeled the macrophages of the red pulp. Compared to CMA, the monoclonal antibody CD11, which can be used only in frozen sections, stained almost solely the fibrous (extracellular) component of the RM. Because CMA stains the reticulum cells in particular, it is better suited to visualize the stromal architecture of splenic white pulp than the monoclonal antibody. Because CMA can be applied to paraffin-embedded material, it is a particularly useful tool to study the splenic stromal architecture in archival material.
KW - Animals
KW - Carbohydrates
KW - Female
KW - Histocytochemistry
KW - Iron
KW - Lectins
KW - Lymphocytes
KW - Macrophages
KW - Male
KW - Rats
KW - Rats, Wistar
KW - Spleen
KW - Staining and Labeling
M3 - SCORING: Journal article
C2 - 10858269
VL - 48
SP - 923
EP - 931
JO - J HISTOCHEM CYTOCHEM
JF - J HISTOCHEM CYTOCHEM
SN - 0022-1554
IS - 7
ER -