Research ExplorerPublicationsLamina propria plasma cells in inflammatory bowel disease: i...

Lamina propria plasma cells in inflammatory bowel disease: intracellular detection of immunoglobulins using flow cytometry

Standard

Lamina propria plasma cells in inflammatory bowel disease: intracellular detection of immunoglobulins using flow cytometry. / Dorn, Isabel; Schlenke, Peter; Mascher, Beate; Stange, Eduard Friedrich; Seyfarth, Michael.

In: IMMUNOBIOLOGY, Vol. 206, No. 5, 12.2002, p. 546-57.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Dorn, I, Schlenke, P, Mascher, B, Stange, EF & Seyfarth, M 2002, 'Lamina propria plasma cells in inflammatory bowel disease: intracellular detection of immunoglobulins using flow cytometry', IMMUNOBIOLOGY, vol. 206, no. 5, pp. 546-57. https://doi.org/10.1078/0171-2985-00203

APA

Dorn, I., Schlenke, P., Mascher, B., Stange, E. F., & Seyfarth, M. (2002). Lamina propria plasma cells in inflammatory bowel disease: intracellular detection of immunoglobulins using flow cytometry. IMMUNOBIOLOGY, 206(5), 546-57. https://doi.org/10.1078/0171-2985-00203

Vancouver

Dorn I, Schlenke P, Mascher B, Stange EF, Seyfarth M. Lamina propria plasma cells in inflammatory bowel disease: intracellular detection of immunoglobulins using flow cytometry. IMMUNOBIOLOGY. 2002 Dec;206(5):546-57. https://doi.org/10.1078/0171-2985-00203

Bibtex

@article{711efa50aab445c986962da4f7adca14,
title = "Lamina propria plasma cells in inflammatory bowel disease: intracellular detection of immunoglobulins using flow cytometry",
abstract = "This is the first application of flow cytometry for the detection of lamina propria plasma cells and their intracellular immunoglobulins in patients with inflammatory bowel disease compared to healthy controls. The study has been focused on the distribution of IgA, IgG, IgM and the four IgG subclasses. Plasma cells were detected as high CD38 positive cells. For fixation and permeabilisation a single step reagent, Ortho Permeafix, was used. By flow cytometry, in patients with inflammatory bowel disease compared to healthy controls, a higher percentage of IgG+ cells can be observed, in Crohn's disease also a higher percentage of IgM+ cells. Regarding the IgG subclass distribution, patients with Crohn's disease show an increase in IgG2+ cells, patients with ulcerative colitis an increase in IgG1+ and IgG3+ cells. These results do agree with and expand the results of earlier immunohistochemical and functional studies, which are favoured today. For the determination of lymphocyte subset proportions and the detection of intracellular antigens, flow cytometry provides a useful alternative to well-established immunohistochemical methods. By analysing a larger number of cells, this method is more reproducible and less prone to interobserver variations than immunohistochemistry, which needs the pre-selection of a mucosal area, the microscopic scoring of a limited number of cells and the circumvention of high background staining. The optimized flow cytometric protocol used in this study might be a promising tool for further investigations of various purposes.",
keywords = "ADP-ribosyl Cyclase/metabolism, ADP-ribosyl Cyclase 1, Adult, Antigens, CD/metabolism, Colon/immunology, Female, Flow Cytometry/methods, Humans, Immunoglobulins/analysis, Inflammatory Bowel Diseases/immunology, Intestinal Mucosa/immunology, Intracellular Fluid/chemistry, Male, Membrane Glycoproteins, Middle Aged, Plasma Cells/chemistry",
author = "Isabel Dorn and Peter Schlenke and Beate Mascher and Stange, {Eduard Friedrich} and Michael Seyfarth",
year = "2002",
month = dec,
doi = "10.1078/0171-2985-00203",
language = "English",
volume = "206",
pages = "546--57",
journal = "IMMUNOBIOLOGY",
issn = "0171-2985",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "5",

}

RIS

TY - JOUR

T1 - Lamina propria plasma cells in inflammatory bowel disease: intracellular detection of immunoglobulins using flow cytometry

AU - Dorn, Isabel

AU - Schlenke, Peter

AU - Mascher, Beate

AU - Stange, Eduard Friedrich

AU - Seyfarth, Michael

PY - 2002/12

Y1 - 2002/12

N2 - This is the first application of flow cytometry for the detection of lamina propria plasma cells and their intracellular immunoglobulins in patients with inflammatory bowel disease compared to healthy controls. The study has been focused on the distribution of IgA, IgG, IgM and the four IgG subclasses. Plasma cells were detected as high CD38 positive cells. For fixation and permeabilisation a single step reagent, Ortho Permeafix, was used. By flow cytometry, in patients with inflammatory bowel disease compared to healthy controls, a higher percentage of IgG+ cells can be observed, in Crohn's disease also a higher percentage of IgM+ cells. Regarding the IgG subclass distribution, patients with Crohn's disease show an increase in IgG2+ cells, patients with ulcerative colitis an increase in IgG1+ and IgG3+ cells. These results do agree with and expand the results of earlier immunohistochemical and functional studies, which are favoured today. For the determination of lymphocyte subset proportions and the detection of intracellular antigens, flow cytometry provides a useful alternative to well-established immunohistochemical methods. By analysing a larger number of cells, this method is more reproducible and less prone to interobserver variations than immunohistochemistry, which needs the pre-selection of a mucosal area, the microscopic scoring of a limited number of cells and the circumvention of high background staining. The optimized flow cytometric protocol used in this study might be a promising tool for further investigations of various purposes.

AB - This is the first application of flow cytometry for the detection of lamina propria plasma cells and their intracellular immunoglobulins in patients with inflammatory bowel disease compared to healthy controls. The study has been focused on the distribution of IgA, IgG, IgM and the four IgG subclasses. Plasma cells were detected as high CD38 positive cells. For fixation and permeabilisation a single step reagent, Ortho Permeafix, was used. By flow cytometry, in patients with inflammatory bowel disease compared to healthy controls, a higher percentage of IgG+ cells can be observed, in Crohn's disease also a higher percentage of IgM+ cells. Regarding the IgG subclass distribution, patients with Crohn's disease show an increase in IgG2+ cells, patients with ulcerative colitis an increase in IgG1+ and IgG3+ cells. These results do agree with and expand the results of earlier immunohistochemical and functional studies, which are favoured today. For the determination of lymphocyte subset proportions and the detection of intracellular antigens, flow cytometry provides a useful alternative to well-established immunohistochemical methods. By analysing a larger number of cells, this method is more reproducible and less prone to interobserver variations than immunohistochemistry, which needs the pre-selection of a mucosal area, the microscopic scoring of a limited number of cells and the circumvention of high background staining. The optimized flow cytometric protocol used in this study might be a promising tool for further investigations of various purposes.

KW - ADP-ribosyl Cyclase/metabolism

KW - ADP-ribosyl Cyclase 1

KW - Adult

KW - Antigens, CD/metabolism

KW - Colon/immunology

KW - Female

KW - Flow Cytometry/methods

KW - Humans

KW - Immunoglobulins/analysis

KW - Inflammatory Bowel Diseases/immunology

KW - Intestinal Mucosa/immunology

KW - Intracellular Fluid/chemistry

KW - Male

KW - Membrane Glycoproteins

KW - Middle Aged

KW - Plasma Cells/chemistry

U2 - 10.1078/0171-2985-00203

DO - 10.1078/0171-2985-00203

M3 - SCORING: Journal article

C2 - 12607730

VL - 206

SP - 546

EP - 557

JO - IMMUNOBIOLOGY

JF - IMMUNOBIOLOGY

SN - 0171-2985

IS - 5

ER -