LAD-ligation: a murine model of myocardial infarction
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LAD-ligation: a murine model of myocardial infarction. / Kolk, Mandy V V; Meyberg, Danja; Deuse, Tobias; Tang-Quan, Karis R; Robbins, Robert C; Reichenspurner, Hermann; Schrepfer, Sonja.
In: JOVE-J VIS EXP, No. 32, 14.10.2009.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - LAD-ligation: a murine model of myocardial infarction
AU - Kolk, Mandy V V
AU - Meyberg, Danja
AU - Deuse, Tobias
AU - Tang-Quan, Karis R
AU - Robbins, Robert C
AU - Reichenspurner, Hermann
AU - Schrepfer, Sonja
PY - 2009/10/14
Y1 - 2009/10/14
N2 - Research models of infarction and myocardial ischemia are essential to investigate the acute and chronic pathobiological and pathophysiological processes in myocardial ischemia and to develop and optimize future treatment. Two different methods of creating myocardial ischemia are performed in laboratory rodents. The first method is to create cryo infarction, a fast but inaccurate technique, where a cryo-pen is applied on the surface of the heart (1-3). Using this method the scientist can not guarantee that the cryo-scar leads to ischemia, also a vast myocardial injury is created that shows pathophysiological side effects that are not related to myocardial infarction. The second method is the permanent ligation of the left anterior descending artery (LAD). Here the LAD is ligated with one single stitch, forming an ischemia that can be seen almost immediately. By closing the LAD, no further blood flow is permitted in that area, while the surrounding myocardial tissue is nearly not affected. This surgical procedure imitates the pathobiological and pathophysiological aspects occurring in infarction-related myocardial ischemia. The method introduced in this video demonstrates the surgical procedure of a mouse infarction model by ligating the LAD. This model is convenient for pathobiological and pathophysiological as well as immunobiological studies on cardiac infarction. The shown technique provides high accuracy and correlates well with histological sections.
AB - Research models of infarction and myocardial ischemia are essential to investigate the acute and chronic pathobiological and pathophysiological processes in myocardial ischemia and to develop and optimize future treatment. Two different methods of creating myocardial ischemia are performed in laboratory rodents. The first method is to create cryo infarction, a fast but inaccurate technique, where a cryo-pen is applied on the surface of the heart (1-3). Using this method the scientist can not guarantee that the cryo-scar leads to ischemia, also a vast myocardial injury is created that shows pathophysiological side effects that are not related to myocardial infarction. The second method is the permanent ligation of the left anterior descending artery (LAD). Here the LAD is ligated with one single stitch, forming an ischemia that can be seen almost immediately. By closing the LAD, no further blood flow is permitted in that area, while the surrounding myocardial tissue is nearly not affected. This surgical procedure imitates the pathobiological and pathophysiological aspects occurring in infarction-related myocardial ischemia. The method introduced in this video demonstrates the surgical procedure of a mouse infarction model by ligating the LAD. This model is convenient for pathobiological and pathophysiological as well as immunobiological studies on cardiac infarction. The shown technique provides high accuracy and correlates well with histological sections.
KW - Animals
KW - Coronary Vessels/surgery
KW - Disease Models, Animal
KW - Ligation/methods
KW - Mice
KW - Mice, Inbred BALB C
KW - Myocardial Infarction
U2 - 10.3791/1438
DO - 10.3791/1438
M3 - SCORING: Journal article
C2 - 19829290
JO - JOVE-J VIS EXP
JF - JOVE-J VIS EXP
SN - 1940-087X
IS - 32
ER -