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L1CAM increases MAP2 expression via the MAPK pathway to promote neurite outgrowth.

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L1CAM increases MAP2 expression via the MAPK pathway to promote neurite outgrowth. / Poplawski, Gunnar Heiko Dirk; Tranziska, Ann-Kathrin; Leshchyns´ka, Iryna; Meier, Ingo Dunya; Streichert, Thomas; Sytnyk, Vladimir; Schachner, Melitta.

In: MOL CELL NEUROSCI, Vol. 50, No. 2, 2, 2012, p. 169-178.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Poplawski, GHD, Tranziska, A-K, Leshchyns´ka, I, Meier, ID, Streichert, T, Sytnyk, V & Schachner, M 2012, 'L1CAM increases MAP2 expression via the MAPK pathway to promote neurite outgrowth.', MOL CELL NEUROSCI, vol. 50, no. 2, 2, pp. 169-178. <http://www.ncbi.nlm.nih.gov/pubmed/22503709?dopt=Citation>

APA

Poplawski, G. H. D., Tranziska, A-K., Leshchyns´ka, I., Meier, I. D., Streichert, T., Sytnyk, V., & Schachner, M. (2012). L1CAM increases MAP2 expression via the MAPK pathway to promote neurite outgrowth. MOL CELL NEUROSCI, 50(2), 169-178. [2]. http://www.ncbi.nlm.nih.gov/pubmed/22503709?dopt=Citation

Vancouver

Poplawski GHD, Tranziska A-K, Leshchyns´ka I, Meier ID, Streichert T, Sytnyk V et al. L1CAM increases MAP2 expression via the MAPK pathway to promote neurite outgrowth. MOL CELL NEUROSCI. 2012;50(2):169-178. 2.

Bibtex

@article{35b7104a120744b98dffd4e84d948888,
title = "L1CAM increases MAP2 expression via the MAPK pathway to promote neurite outgrowth.",
abstract = "The neural cell adhesion molecule L1 (L1CAM) promotes neurite outgrowth via mechanisms that are not completely understood, but are known to involve the cytoskeleton. Here, we show that L1 binds directly to the microtubule associated protein 2c (MAP2c). This isoform of MAP2 is predominantly expressed in developing neurons. We found that the mRNA and protein levels of MAP2c, but not of MAP2a/b, are reduced in brains of young adult L1-deficient transgenic mice. We show via ELISA, that MAP2c, but not MAP2a/b, binds directly to the intracellular domain of L1. Remarkably, all these MAP2 isoforms co-immunoprecipitate with L1, suggesting that MAP2a/b associates with L1 via intermediate binding partners. The expression levels of MAP2a/b/c correlate with those of L1 in different brain regions of early postnatal mice, while expression levels of heat shock cognate protein 70 (Hsc70) or actin do not. L1 enhances the expression of MAP2a/b/c in cultured hippocampal neurons depending on activation of the mitogen-activated protein kinase (MAPK) pathway. Deficiency in both L1 and MAP2a/b/c expression results in reduced neurite outgrowth in vitro. We propose that the L1-triggered increase in MAP2a/b/c expression is required to promote neurite outgrowth.",
keywords = "Animals, Male, Cells, Cultured, Mice, Mice, Knockout, Heterozygote, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MAP Kinase Signaling System, Mice, Inbred Strains, Neural Cell Adhesion Molecule L1/genetics/*metabolism, Protein Isoforms/metabolism, Brain/growth & development/metabolism, Microtubule-Associated Proteins/biosynthesis/*metabolism, Neurites/enzymology/metabolism/*physiology, Animals, Male, Cells, Cultured, Mice, Mice, Knockout, Heterozygote, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MAP Kinase Signaling System, Mice, Inbred Strains, Neural Cell Adhesion Molecule L1/genetics/*metabolism, Protein Isoforms/metabolism, Brain/growth & development/metabolism, Microtubule-Associated Proteins/biosynthesis/*metabolism, Neurites/enzymology/metabolism/*physiology",
author = "Poplawski, {Gunnar Heiko Dirk} and Ann-Kathrin Tranziska and Iryna Leshchyns´ka and Meier, {Ingo Dunya} and Thomas Streichert and Vladimir Sytnyk and Melitta Schachner",
year = "2012",
language = "English",
volume = "50",
pages = "169--178",
journal = "MOL CELL NEUROSCI",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - L1CAM increases MAP2 expression via the MAPK pathway to promote neurite outgrowth.

AU - Poplawski, Gunnar Heiko Dirk

AU - Tranziska, Ann-Kathrin

AU - Leshchyns´ka, Iryna

AU - Meier, Ingo Dunya

AU - Streichert, Thomas

AU - Sytnyk, Vladimir

AU - Schachner, Melitta

PY - 2012

Y1 - 2012

N2 - The neural cell adhesion molecule L1 (L1CAM) promotes neurite outgrowth via mechanisms that are not completely understood, but are known to involve the cytoskeleton. Here, we show that L1 binds directly to the microtubule associated protein 2c (MAP2c). This isoform of MAP2 is predominantly expressed in developing neurons. We found that the mRNA and protein levels of MAP2c, but not of MAP2a/b, are reduced in brains of young adult L1-deficient transgenic mice. We show via ELISA, that MAP2c, but not MAP2a/b, binds directly to the intracellular domain of L1. Remarkably, all these MAP2 isoforms co-immunoprecipitate with L1, suggesting that MAP2a/b associates with L1 via intermediate binding partners. The expression levels of MAP2a/b/c correlate with those of L1 in different brain regions of early postnatal mice, while expression levels of heat shock cognate protein 70 (Hsc70) or actin do not. L1 enhances the expression of MAP2a/b/c in cultured hippocampal neurons depending on activation of the mitogen-activated protein kinase (MAPK) pathway. Deficiency in both L1 and MAP2a/b/c expression results in reduced neurite outgrowth in vitro. We propose that the L1-triggered increase in MAP2a/b/c expression is required to promote neurite outgrowth.

AB - The neural cell adhesion molecule L1 (L1CAM) promotes neurite outgrowth via mechanisms that are not completely understood, but are known to involve the cytoskeleton. Here, we show that L1 binds directly to the microtubule associated protein 2c (MAP2c). This isoform of MAP2 is predominantly expressed in developing neurons. We found that the mRNA and protein levels of MAP2c, but not of MAP2a/b, are reduced in brains of young adult L1-deficient transgenic mice. We show via ELISA, that MAP2c, but not MAP2a/b, binds directly to the intracellular domain of L1. Remarkably, all these MAP2 isoforms co-immunoprecipitate with L1, suggesting that MAP2a/b associates with L1 via intermediate binding partners. The expression levels of MAP2a/b/c correlate with those of L1 in different brain regions of early postnatal mice, while expression levels of heat shock cognate protein 70 (Hsc70) or actin do not. L1 enhances the expression of MAP2a/b/c in cultured hippocampal neurons depending on activation of the mitogen-activated protein kinase (MAPK) pathway. Deficiency in both L1 and MAP2a/b/c expression results in reduced neurite outgrowth in vitro. We propose that the L1-triggered increase in MAP2a/b/c expression is required to promote neurite outgrowth.

KW - Animals

KW - Male

KW - Cells, Cultured

KW - Mice

KW - Mice, Knockout

KW - Heterozygote

KW - Oligonucleotide Array Sequence Analysis

KW - Gene Expression Profiling

KW - MAP Kinase Signaling System

KW - Mice, Inbred Strains

KW - Neural Cell Adhesion Molecule L1/genetics/metabolism

KW - Protein Isoforms/metabolism

KW - Brain/growth & development/metabolism

KW - Microtubule-Associated Proteins/biosynthesis/metabolism

KW - Neurites/enzymology/metabolism/physiology

KW - Animals

KW - Male

KW - Cells, Cultured

KW - Mice

KW - Mice, Knockout

KW - Heterozygote

KW - Oligonucleotide Array Sequence Analysis

KW - Gene Expression Profiling

KW - MAP Kinase Signaling System

KW - Mice, Inbred Strains

KW - Neural Cell Adhesion Molecule L1/genetics/metabolism

KW - Protein Isoforms/metabolism

KW - Brain/growth & development/metabolism

KW - Microtubule-Associated Proteins/biosynthesis/metabolism

KW - Neurites/enzymology/metabolism/physiology

M3 - SCORING: Journal article

VL - 50

SP - 169

EP - 178

JO - MOL CELL NEUROSCI

JF - MOL CELL NEUROSCI

SN - 1044-7431

IS - 2

M1 - 2

ER -