Ketogenic diet attenuates NMDA-induced damage to rat's retinal ganglion cells in an age-dependent manner
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Ketogenic diet attenuates NMDA-induced damage to rat's retinal ganglion cells in an age-dependent manner. / Zarnowski, Tomasz; Choragiewicz, Tomasz J; Schuettauf, Frank; Zrenner, Eberhart; Rejdak, Robert; Gasior, Maciej; Zarnowska, Iwona; Thaler, Sebastian.
In: OPHTHALMIC RES, Vol. 53, No. 3, 2015, p. 162-7.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Ketogenic diet attenuates NMDA-induced damage to rat's retinal ganglion cells in an age-dependent manner
AU - Zarnowski, Tomasz
AU - Choragiewicz, Tomasz J
AU - Schuettauf, Frank
AU - Zrenner, Eberhart
AU - Rejdak, Robert
AU - Gasior, Maciej
AU - Zarnowska, Iwona
AU - Thaler, Sebastian
N1 - © 2015 S. Karger AG, Basel.
PY - 2015
Y1 - 2015
N2 - OBJECTIVE: This study was conducted to investigate neuroprotective effects of a high fat/low carbohydrate and protein diet (ketogenic diet, KD) in a model of N-methyl D-aspartate (NMDA)-induced retinal ganglion cell (RGC) damage in juvenile and young adult rats.METHODS: Juvenile (30-35 days old) and young adult (56-70 days old) female Brown Norway rats were fed the KD for 21 days; rats exposed to a standard rodent diet (SRD) served as controls. The main constituents of the KD used in the present study were approximately 80% fats, 8% proteins, and less than 1% carbohydrates. On day 14 of exposure to the KD (or the SRD in the control group), each rat received a single intravitreal injection of NMDA; RGCs were then retrogradely labelled by hydroxystilbamidine on day 19 and collected on day 21 to assess the degree of damage induced by NMDA. Blood biomarkers to confirm the expected metabolic response to the KD (i.e. ketosis and hypoglycaemia) were also assessed.RESULTS: Although both the juvenile and young adult rats developed comparable ketosis and hypoglycaemia when fed the KD, NMDA-induced loss in RGCs was significantly attenuated only in juvenile rats exposed to the KD in comparison with those fed the SRD; exposure to the KD had no protective effect in young adult rats. In summary, exposure to the KD had a neuroprotective effect in NMDA-induced RGC damage in juvenile rats, but not in young adult rats.CONCLUSION: These results support further exploration of metabolic interventions to treat optic neuropathies associated with neurodegeneration.
AB - OBJECTIVE: This study was conducted to investigate neuroprotective effects of a high fat/low carbohydrate and protein diet (ketogenic diet, KD) in a model of N-methyl D-aspartate (NMDA)-induced retinal ganglion cell (RGC) damage in juvenile and young adult rats.METHODS: Juvenile (30-35 days old) and young adult (56-70 days old) female Brown Norway rats were fed the KD for 21 days; rats exposed to a standard rodent diet (SRD) served as controls. The main constituents of the KD used in the present study were approximately 80% fats, 8% proteins, and less than 1% carbohydrates. On day 14 of exposure to the KD (or the SRD in the control group), each rat received a single intravitreal injection of NMDA; RGCs were then retrogradely labelled by hydroxystilbamidine on day 19 and collected on day 21 to assess the degree of damage induced by NMDA. Blood biomarkers to confirm the expected metabolic response to the KD (i.e. ketosis and hypoglycaemia) were also assessed.RESULTS: Although both the juvenile and young adult rats developed comparable ketosis and hypoglycaemia when fed the KD, NMDA-induced loss in RGCs was significantly attenuated only in juvenile rats exposed to the KD in comparison with those fed the SRD; exposure to the KD had no protective effect in young adult rats. In summary, exposure to the KD had a neuroprotective effect in NMDA-induced RGC damage in juvenile rats, but not in young adult rats.CONCLUSION: These results support further exploration of metabolic interventions to treat optic neuropathies associated with neurodegeneration.
KW - Animals
KW - Cell Count
KW - D-Aspartic Acid/metabolism
KW - Diet Therapy/methods
KW - Diet, Ketogenic
KW - Disease Models, Animal
KW - Female
KW - N-Methylaspartate/toxicity
KW - Neuroprotective Agents/administration & dosage
KW - Rats
KW - Rats, Inbred BN
KW - Retinal Degeneration/chemically induced
KW - Retinal Ganglion Cells/cytology
U2 - 10.1159/000379753
DO - 10.1159/000379753
M3 - SCORING: Journal article
C2 - 25832811
VL - 53
SP - 162
EP - 167
JO - OPHTHALMIC RES
JF - OPHTHALMIC RES
SN - 0030-3747
IS - 3
ER -