Keratins are novel markers of renal epithelial cell injury

Sonja Djudjaj; Marios Papasotiriou; Roman D Bülow; Alexandra Wagnerova; Maja T Lindenmeyer; Clemens D Cohen; Pavel Strnad; Dimitrios S Goumenos; Jürgen Floege; Peter Boor

doi:10.1016/j.kint.2015.10.015

Keratins are novel markers of renal epithelial cell injury

Standard

Keratins are novel markers of renal epithelial cell injury. / Djudjaj, Sonja; Papasotiriou, Marios; Bülow, Roman D; Wagnerova, Alexandra; Lindenmeyer, Maja T; Cohen, Clemens D; Strnad, Pavel; Goumenos, Dimitrios S; Floege, Jürgen; Boor, Peter.

In: KIDNEY INT, Vol. 89, No. 4, 04.2016, p. 792-808.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Djudjaj, S, Papasotiriou, M, Bülow, RD, Wagnerova, A, Lindenmeyer, MT, Cohen, CD, Strnad, P, Goumenos, DS, Floege, J & Boor, P 2016, 'Keratins are novel markers of renal epithelial cell injury', KIDNEY INT, vol. 89, no. 4, pp. 792-808. https://doi.org/10.1016/j.kint.2015.10.015

APA

Djudjaj, S., Papasotiriou, M., Bülow, R. D., Wagnerova, A., Lindenmeyer, M. T., Cohen, C. D., Strnad, P., Goumenos, D. S., Floege, J., & Boor, P. (2016). Keratins are novel markers of renal epithelial cell injury. KIDNEY INT, 89(4), 792-808. https://doi.org/10.1016/j.kint.2015.10.015

Vancouver

Djudjaj S, Papasotiriou M, Bülow RD, Wagnerova A, Lindenmeyer MT, Cohen CD et al. Keratins are novel markers of renal epithelial cell injury. KIDNEY INT. 2016 Apr;89(4):792-808. https://doi.org/10.1016/j.kint.2015.10.015

Bibtex

@article{d79bafd6c4a641438c60931ae50a3842,

title = "Keratins are novel markers of renal epithelial cell injury",

abstract = "Keratins, the intermediate filaments of the epithelial cell cytoskeleton, are up-regulated and post-translationally modified in stress situations. Renal tubular epithelial cell stress is a common finding in progressive kidney diseases, but little is known about keratin expression and phosphorylation. Here, we comprehensively describe keratin expression in healthy and diseased kidneys. In healthy mice, the major renal keratins, K7, K8, K18, and K19, were expressed in the collecting ducts and K8, K18 in the glomerular parietal epithelial cells. Tubular expression of all 4 keratins increased by 20- to 40-fold in 5 different models of renal tubular injury as assessed by immunohistochemistry, Western blot, and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The up-regulation became significant early after disease induction, increased with disease progression, was found de novo in distal tubules and was accompanied by altered subcellular localization. Phosphorylation of K8 and K18 increased under stress. In humans, injured tubules also exhibited increased keratin expression. Urinary K18 was only detected in mice and patients with tubular cell injury. Keratins labeled glomerular parietal epithelial cells forming crescents in patients and animals. Thus, all 4 major renal keratins are significantly, early, and progressively up-regulated upon tubular injury regardless of the underlying disease and may be novel sensitive markers of renal tubular cell stress. ",

keywords = "Adult, Aged, Aged, 80 and over, Animals, Biomarkers, Case-Control Studies, Epithelial Cells, Female, Humans, Keratin-18, Keratins, Kidney, Kidney Diseases, Male, Mice, Inbred C57BL, Phosphorylation, Ureteral Obstruction, Journal Article, Research Support, Non-U.S. Gov't",

author = "Sonja Djudjaj and Marios Papasotiriou and B{\"u}low, {Roman D} and Alexandra Wagnerova and Lindenmeyer, {Maja T} and Cohen, {Clemens D} and Pavel Strnad and Goumenos, {Dimitrios S} and J{\"u}rgen Floege and Peter Boor",

year = "2016",

month = apr,

doi = "10.1016/j.kint.2015.10.015",

language = "English",

volume = "89",

pages = "792--808",

journal = "KIDNEY INT",

issn = "0085-2538",

publisher = "NATURE PUBLISHING GROUP",

number = "4",

}

RIS

TY - JOUR

T1 - Keratins are novel markers of renal epithelial cell injury

AU - Djudjaj, Sonja

AU - Papasotiriou, Marios

AU - Bülow, Roman D

AU - Wagnerova, Alexandra

AU - Lindenmeyer, Maja T

AU - Cohen, Clemens D

AU - Strnad, Pavel

AU - Goumenos, Dimitrios S

AU - Floege, Jürgen

AU - Boor, Peter

PY - 2016/4

Y1 - 2016/4

N2 - Keratins, the intermediate filaments of the epithelial cell cytoskeleton, are up-regulated and post-translationally modified in stress situations. Renal tubular epithelial cell stress is a common finding in progressive kidney diseases, but little is known about keratin expression and phosphorylation. Here, we comprehensively describe keratin expression in healthy and diseased kidneys. In healthy mice, the major renal keratins, K7, K8, K18, and K19, were expressed in the collecting ducts and K8, K18 in the glomerular parietal epithelial cells. Tubular expression of all 4 keratins increased by 20- to 40-fold in 5 different models of renal tubular injury as assessed by immunohistochemistry, Western blot, and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The up-regulation became significant early after disease induction, increased with disease progression, was found de novo in distal tubules and was accompanied by altered subcellular localization. Phosphorylation of K8 and K18 increased under stress. In humans, injured tubules also exhibited increased keratin expression. Urinary K18 was only detected in mice and patients with tubular cell injury. Keratins labeled glomerular parietal epithelial cells forming crescents in patients and animals. Thus, all 4 major renal keratins are significantly, early, and progressively up-regulated upon tubular injury regardless of the underlying disease and may be novel sensitive markers of renal tubular cell stress.

AB - Keratins, the intermediate filaments of the epithelial cell cytoskeleton, are up-regulated and post-translationally modified in stress situations. Renal tubular epithelial cell stress is a common finding in progressive kidney diseases, but little is known about keratin expression and phosphorylation. Here, we comprehensively describe keratin expression in healthy and diseased kidneys. In healthy mice, the major renal keratins, K7, K8, K18, and K19, were expressed in the collecting ducts and K8, K18 in the glomerular parietal epithelial cells. Tubular expression of all 4 keratins increased by 20- to 40-fold in 5 different models of renal tubular injury as assessed by immunohistochemistry, Western blot, and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The up-regulation became significant early after disease induction, increased with disease progression, was found de novo in distal tubules and was accompanied by altered subcellular localization. Phosphorylation of K8 and K18 increased under stress. In humans, injured tubules also exhibited increased keratin expression. Urinary K18 was only detected in mice and patients with tubular cell injury. Keratins labeled glomerular parietal epithelial cells forming crescents in patients and animals. Thus, all 4 major renal keratins are significantly, early, and progressively up-regulated upon tubular injury regardless of the underlying disease and may be novel sensitive markers of renal tubular cell stress.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Animals

KW - Biomarkers

KW - Case-Control Studies

KW - Epithelial Cells

KW - Female

KW - Humans

KW - Keratin-18

KW - Keratins

KW - Kidney

KW - Kidney Diseases

KW - Male

KW - Mice, Inbred C57BL

KW - Phosphorylation

KW - Ureteral Obstruction

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.kint.2015.10.015

DO - 10.1016/j.kint.2015.10.015

M3 - SCORING: Journal article

C2 - 26924053

VL - 89

SP - 792

EP - 808

JO - KIDNEY INT

JF - KIDNEY INT

SN - 0085-2538

IS - 4

ER -