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Keratinocyte-specific onset of serine protease BSSP expression in experimental carcinogenesis

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Keratinocyte-specific onset of serine protease BSSP expression in experimental carcinogenesis. / Breitenbach, U; Tuckermann, J P; Gebhardt, C; Richter, K H; Fürstenberger, G; Christofori, G; Angel, P.

In: J INVEST DERMATOL, Vol. 117, No. 3, 09.2001, p. 634-40.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Breitenbach, U, Tuckermann, JP, Gebhardt, C, Richter, KH, Fürstenberger, G, Christofori, G & Angel, P 2001, 'Keratinocyte-specific onset of serine protease BSSP expression in experimental carcinogenesis', J INVEST DERMATOL, vol. 117, no. 3, pp. 634-40. https://doi.org/10.1046/j.0022-202x.2001.01437.x

APA

Breitenbach, U., Tuckermann, J. P., Gebhardt, C., Richter, K. H., Fürstenberger, G., Christofori, G., & Angel, P. (2001). Keratinocyte-specific onset of serine protease BSSP expression in experimental carcinogenesis. J INVEST DERMATOL, 117(3), 634-40. https://doi.org/10.1046/j.0022-202x.2001.01437.x

Vancouver

Breitenbach U, Tuckermann JP, Gebhardt C, Richter KH, Fürstenberger G, Christofori G et al. Keratinocyte-specific onset of serine protease BSSP expression in experimental carcinogenesis. J INVEST DERMATOL. 2001 Sep;117(3):634-40. https://doi.org/10.1046/j.0022-202x.2001.01437.x

Bibtex

@article{b3d728d4384e4963bb994fcb51e86b47,
title = "Keratinocyte-specific onset of serine protease BSSP expression in experimental carcinogenesis",
abstract = "Malignant transformation of mouse skin by chemical carcinogens and tumor promoters, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate, is a multistage process leading to the formation of squamous cell carcinomas. In an effort to identify target genes whose expression is associated with skin tumorigenesis we combined elements of suppression subtractive hybridization with differential screening to isolate genes that are differentially upregulated in mouse skin after short-term treatment with 12-O-tetradecanoylphorbol-13-acetate and that exhibit a high constitutive expression in squamous cell carcinomas. Here, we report the detailed analysis of one of these cDNAs encoding the serine protease BSSP in mouse skin. Phorbol ester application increases BSSP expression in keratinocytes of the epidermis and the hair follicle several-fold starting 4 h post- treatment. Transcriptional activation of BSSP by 12-O-tetradecanoylphorbol-13-acetate was found to be independent of c-Fos expression and resistant to downregulation by glucocorticoids. By monitoring BSSP expression throughout experimental skin carcinogenesis we found strong constitutive expression in hyperplastic epidermis as well as in proliferatively active keratinocytes of benign and malignant skin tumors. These results establish a novel link between expression of an as yet ill-defined serine protease and skin carcinogenesis.",
keywords = "Animals, Biomarkers, Tumor, Carcinogens, Female, Kallikreins, Keratinocytes, Mice, Neoplasms, Experimental, Serine Endopeptidases, Signal Transduction, Skin Neoplasms, Tetradecanoylphorbol Acetate, Journal Article, Research Support, Non-U.S. Gov't",
author = "U Breitenbach and Tuckermann, {J P} and C Gebhardt and Richter, {K H} and G F{\"u}rstenberger and G Christofori and P Angel",
year = "2001",
month = sep,
doi = "10.1046/j.0022-202x.2001.01437.x",
language = "English",
volume = "117",
pages = "634--40",
journal = "J INVEST DERMATOL",
issn = "0022-202X",
publisher = "NATURE PUBLISHING GROUP",
number = "3",

}

RIS

TY - JOUR

T1 - Keratinocyte-specific onset of serine protease BSSP expression in experimental carcinogenesis

AU - Breitenbach, U

AU - Tuckermann, J P

AU - Gebhardt, C

AU - Richter, K H

AU - Fürstenberger, G

AU - Christofori, G

AU - Angel, P

PY - 2001/9

Y1 - 2001/9

N2 - Malignant transformation of mouse skin by chemical carcinogens and tumor promoters, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate, is a multistage process leading to the formation of squamous cell carcinomas. In an effort to identify target genes whose expression is associated with skin tumorigenesis we combined elements of suppression subtractive hybridization with differential screening to isolate genes that are differentially upregulated in mouse skin after short-term treatment with 12-O-tetradecanoylphorbol-13-acetate and that exhibit a high constitutive expression in squamous cell carcinomas. Here, we report the detailed analysis of one of these cDNAs encoding the serine protease BSSP in mouse skin. Phorbol ester application increases BSSP expression in keratinocytes of the epidermis and the hair follicle several-fold starting 4 h post- treatment. Transcriptional activation of BSSP by 12-O-tetradecanoylphorbol-13-acetate was found to be independent of c-Fos expression and resistant to downregulation by glucocorticoids. By monitoring BSSP expression throughout experimental skin carcinogenesis we found strong constitutive expression in hyperplastic epidermis as well as in proliferatively active keratinocytes of benign and malignant skin tumors. These results establish a novel link between expression of an as yet ill-defined serine protease and skin carcinogenesis.

AB - Malignant transformation of mouse skin by chemical carcinogens and tumor promoters, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate, is a multistage process leading to the formation of squamous cell carcinomas. In an effort to identify target genes whose expression is associated with skin tumorigenesis we combined elements of suppression subtractive hybridization with differential screening to isolate genes that are differentially upregulated in mouse skin after short-term treatment with 12-O-tetradecanoylphorbol-13-acetate and that exhibit a high constitutive expression in squamous cell carcinomas. Here, we report the detailed analysis of one of these cDNAs encoding the serine protease BSSP in mouse skin. Phorbol ester application increases BSSP expression in keratinocytes of the epidermis and the hair follicle several-fold starting 4 h post- treatment. Transcriptional activation of BSSP by 12-O-tetradecanoylphorbol-13-acetate was found to be independent of c-Fos expression and resistant to downregulation by glucocorticoids. By monitoring BSSP expression throughout experimental skin carcinogenesis we found strong constitutive expression in hyperplastic epidermis as well as in proliferatively active keratinocytes of benign and malignant skin tumors. These results establish a novel link between expression of an as yet ill-defined serine protease and skin carcinogenesis.

KW - Animals

KW - Biomarkers, Tumor

KW - Carcinogens

KW - Female

KW - Kallikreins

KW - Keratinocytes

KW - Mice

KW - Neoplasms, Experimental

KW - Serine Endopeptidases

KW - Signal Transduction

KW - Skin Neoplasms

KW - Tetradecanoylphorbol Acetate

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1046/j.0022-202x.2001.01437.x

DO - 10.1046/j.0022-202x.2001.01437.x

M3 - SCORING: Journal article

C2 - 11564170

VL - 117

SP - 634

EP - 640

JO - J INVEST DERMATOL

JF - J INVEST DERMATOL

SN - 0022-202X

IS - 3

ER -