Involvement of single nucleotide polymorphisms in ovarian poor response
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Involvement of single nucleotide polymorphisms in ovarian poor response. / Ghaderian, Sayyed Mohammad Hossein; Akbarzadeh, Reza; Salehpour, Saghar.
In: J ASSIST REPROD GEN, Vol. 38, No. 9, 09.2021, p. 2405-2413.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Involvement of single nucleotide polymorphisms in ovarian poor response
AU - Ghaderian, Sayyed Mohammad Hossein
AU - Akbarzadeh, Reza
AU - Salehpour, Saghar
N1 - © 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021/9
Y1 - 2021/9
N2 - PURPOSE: Unpredictability in acquiring an adequate number of high-quality oocytes following ovarian stimulation is one of the major complications in controlled ovarian hyperstimulation (COH). Genetic predispositions of variations could alter the immunological profiles and consequently be implicated in the variability of ovarian response to the stimulation.DESIGN: Uncovering the influence of variations in AMHR2, LHCGR, MTHFR, PGR, and SERPINE1 genes with ovarian response to gonadotrophin stimulation in COH of infertile women.METHODS: Blood samples of the women with a good ovarian response (GOR) or with a poor ovarian response (POR) were collected. Genomic DNA was extracted, and gene variations were genotyped by TaqMan SNP Genotyping Assays using primer-probe sets or real-time PCR Kit.RESULTS: Except for PGR (rs10895068), allele distributions demonstrate that the majority of POR patients carried minor alleles of AMHR2 (rs2002555, G-allele), LHCGR (rs2293275, G-allele), MTHFR (rs1801131, C-allele, and rs1801133, T-allele), and SERPINE1 (rs1799889, 4G allele) genes compared to the GOR. Similarly, genotypes with a minor allele in AMHR2, LHCGR, MTHFR, and SERPINE1 genes had a higher prevalence among POR patients with the polymorphic genotypes. However, further genotype stratification indicated that the minor alleles of these genes are not associated with poor response. Multivariate logistic analysis of clinical-demographic factors and polymorphic genotypes demonstrated a correlation between FSH levels and polymorphic genotypes of SERPINE1 in poor response status.CONCLUSIONS: Despite a higher prevalence of AMHR2, LHCGR, MTHFR, and SERPINE1 variations in the patients with poor ovarian response, it seems that these variations are not associated with the ovarian response.
AB - PURPOSE: Unpredictability in acquiring an adequate number of high-quality oocytes following ovarian stimulation is one of the major complications in controlled ovarian hyperstimulation (COH). Genetic predispositions of variations could alter the immunological profiles and consequently be implicated in the variability of ovarian response to the stimulation.DESIGN: Uncovering the influence of variations in AMHR2, LHCGR, MTHFR, PGR, and SERPINE1 genes with ovarian response to gonadotrophin stimulation in COH of infertile women.METHODS: Blood samples of the women with a good ovarian response (GOR) or with a poor ovarian response (POR) were collected. Genomic DNA was extracted, and gene variations were genotyped by TaqMan SNP Genotyping Assays using primer-probe sets or real-time PCR Kit.RESULTS: Except for PGR (rs10895068), allele distributions demonstrate that the majority of POR patients carried minor alleles of AMHR2 (rs2002555, G-allele), LHCGR (rs2293275, G-allele), MTHFR (rs1801131, C-allele, and rs1801133, T-allele), and SERPINE1 (rs1799889, 4G allele) genes compared to the GOR. Similarly, genotypes with a minor allele in AMHR2, LHCGR, MTHFR, and SERPINE1 genes had a higher prevalence among POR patients with the polymorphic genotypes. However, further genotype stratification indicated that the minor alleles of these genes are not associated with poor response. Multivariate logistic analysis of clinical-demographic factors and polymorphic genotypes demonstrated a correlation between FSH levels and polymorphic genotypes of SERPINE1 in poor response status.CONCLUSIONS: Despite a higher prevalence of AMHR2, LHCGR, MTHFR, and SERPINE1 variations in the patients with poor ovarian response, it seems that these variations are not associated with the ovarian response.
KW - Adult
KW - Female
KW - Fertilization in Vitro
KW - Genetic Predisposition to Disease
KW - Genotype
KW - Gonadotropins/pharmacology
KW - Humans
KW - Infertility, Female/drug therapy
KW - Intracellular Signaling Peptides and Proteins/genetics
KW - Methylenetetrahydrofolate Reductase (NADPH2)/genetics
KW - Nuclear Proteins/genetics
KW - Ovarian Hyperstimulation Syndrome/genetics
KW - Ovarian Reserve/drug effects
KW - Ovulation Induction/statistics & numerical data
KW - Plasminogen Activator Inhibitor 1/genetics
KW - Polymorphism, Single Nucleotide
KW - RNA, Long Noncoding/genetics
KW - Receptors, LH/genetics
U2 - 10.1007/s10815-021-02242-w
DO - 10.1007/s10815-021-02242-w
M3 - SCORING: Journal article
C2 - 34050449
VL - 38
SP - 2405
EP - 2413
JO - J ASSIST REPROD GEN
JF - J ASSIST REPROD GEN
SN - 1058-0468
IS - 9
ER -