Investigating retinal toxicity of a lutein-based dye in a model of isolated and perfused bovine retina
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Investigating retinal toxicity of a lutein-based dye in a model of isolated and perfused bovine retina. / Mueller, Sebastian; Krupp, Carlo; Schnichels, Sven; Hofmann, Johanna; Spitzer, Martin; Bartz-Schmidt, Karl Ulrich; Szurman, Peter; Januschowski, Kai.
In: GRAEF ARCH CLIN EXP, Vol. 257, No. 5, 05.2019, p. 961-966.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Investigating retinal toxicity of a lutein-based dye in a model of isolated and perfused bovine retina
AU - Mueller, Sebastian
AU - Krupp, Carlo
AU - Schnichels, Sven
AU - Hofmann, Johanna
AU - Spitzer, Martin
AU - Bartz-Schmidt, Karl Ulrich
AU - Szurman, Peter
AU - Januschowski, Kai
PY - 2019/5
Y1 - 2019/5
N2 - PURPOSE: Retidyne™ is a new lutein-based dye for internal limiting membrane staining. It uses the intrinsic staining characteristics of lutein which is already known to act as an antioxidant and blue-light filter in the human retina. We investigated retinal tolerance to different staining times measured by the electroretinogram (ERG) of an isolated and perfused retina whole mount.METHODS: For functionality, testing bovine retinas were prepared and perfused with an oxygen saturated standard solution and the ERG was recorded until stable b-wave amplitudes were reached. Then the perfusion was stopped and Retidyne™ was applied directly onto the retinal surface for exposure times of 60 or 120 s. After restarting the perfusion with standard solution, the ERG amplitudes were monitored for 75 min. To investigate the effects on photoreceptor function alone, 1 mM asparate was added to block b-waves.RESULTS: For an exposure time of 60 s amplitudes of a- and b-waves remained stable throughout the experiment. Exposure times of 120 s caused an initial drop of amplitudes that reached statistical significance only for a-waves (a, - 21%, p = 0.047; b, - 14%, p = 0.052). This effect was only seen during the first minutes of the washout and the ERG recovered completely.CONCLUSIONS: In the model of isolated and perfused bovine retina, Retidyne™ showed a good safety profile for common intraoperatively used staining times. An initial toxic effect regarding the transient drop of amplitudes cannot be ruled out but the effect might also be explained by the partial blockage of the flashlight due to a more intense staining effect at the beginning of the washout.
AB - PURPOSE: Retidyne™ is a new lutein-based dye for internal limiting membrane staining. It uses the intrinsic staining characteristics of lutein which is already known to act as an antioxidant and blue-light filter in the human retina. We investigated retinal tolerance to different staining times measured by the electroretinogram (ERG) of an isolated and perfused retina whole mount.METHODS: For functionality, testing bovine retinas were prepared and perfused with an oxygen saturated standard solution and the ERG was recorded until stable b-wave amplitudes were reached. Then the perfusion was stopped and Retidyne™ was applied directly onto the retinal surface for exposure times of 60 or 120 s. After restarting the perfusion with standard solution, the ERG amplitudes were monitored for 75 min. To investigate the effects on photoreceptor function alone, 1 mM asparate was added to block b-waves.RESULTS: For an exposure time of 60 s amplitudes of a- and b-waves remained stable throughout the experiment. Exposure times of 120 s caused an initial drop of amplitudes that reached statistical significance only for a-waves (a, - 21%, p = 0.047; b, - 14%, p = 0.052). This effect was only seen during the first minutes of the washout and the ERG recovered completely.CONCLUSIONS: In the model of isolated and perfused bovine retina, Retidyne™ showed a good safety profile for common intraoperatively used staining times. An initial toxic effect regarding the transient drop of amplitudes cannot be ruled out but the effect might also be explained by the partial blockage of the flashlight due to a more intense staining effect at the beginning of the washout.
KW - Animals
KW - Cattle
KW - Coloring Agents/toxicity
KW - Disease Models, Animal
KW - Electroretinography
KW - Lutein/toxicity
KW - Perfusion
KW - Retinal Diseases/chemically induced
KW - Retinal Ganglion Cells/drug effects
U2 - 10.1007/s00417-019-04260-y
DO - 10.1007/s00417-019-04260-y
M3 - SCORING: Journal article
C2 - 30788607
VL - 257
SP - 961
EP - 966
JO - GRAEF ARCH CLIN EXP
JF - GRAEF ARCH CLIN EXP
SN - 0721-832X
IS - 5
ER -