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Intracellular cytokine repertoire in different T cell subsets from patients with sarcoidosis

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Intracellular cytokine repertoire in different T cell subsets from patients with sarcoidosis. / Möllers, M; Aries, S P; Drömann, D; Mascher, B; Braun, J; Dalhoff, K.

In: THORAX, Vol. 56, No. 6, 06.2001, p. 487-493.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Möllers, M, Aries, SP, Drömann, D, Mascher, B, Braun, J & Dalhoff, K 2001, 'Intracellular cytokine repertoire in different T cell subsets from patients with sarcoidosis', THORAX, vol. 56, no. 6, pp. 487-493. https://doi.org/10.1136/thorax.56.6.487

APA

Möllers, M., Aries, S. P., Drömann, D., Mascher, B., Braun, J., & Dalhoff, K. (2001). Intracellular cytokine repertoire in different T cell subsets from patients with sarcoidosis. THORAX, 56(6), 487-493. https://doi.org/10.1136/thorax.56.6.487

Vancouver

Möllers M, Aries SP, Drömann D, Mascher B, Braun J, Dalhoff K. Intracellular cytokine repertoire in different T cell subsets from patients with sarcoidosis. THORAX. 2001 Jun;56(6):487-493. https://doi.org/10.1136/thorax.56.6.487

Bibtex

@article{a80d446fd2d4495fa0bc725d5c945e19,
title = "Intracellular cytokine repertoire in different T cell subsets from patients with sarcoidosis",
abstract = "BACKGROUND: Pulmonary sarcoidosis is characterised by a mononuclear alveolitis with a predominance of CD4+ T cells and macrophages. We determined the intracellular expression of interferon (IFN)gamma, interleukin (IL)-2, tumour necrosis factor (TNF)alpha, IL-4, IL-5 and IL-10 in CD4+ and CD8+, naive and memory lymphocytes from blood and bronchoalveolar lavage (BAL) fluid using three colour flow cytometry.METHODS: Eighteen untreated patients with pulmonary sarcoidosis were evaluated and stratified according to whether they had acute or chronic disease.RESULTS: Significantly more T cells expressed Th1 than Th2 type cytokines in both BAL fluid and peripheral blood samples, regardless of clinical presentation. Significantly greater proportions of T cells secreted Th1 type cytokines in BAL fluid than in peripheral blood. Th1 type cytokines were more frequently expressed by peripheral and alveolar T cells in acute disease than in chronic disease. There were no significant differences between CD4+ and CD8+ T cells. Concerning naive and memory lymphocytes, significantly higher CD45RO:CD45RA ratios were found in BAL fluid than in blood, and increased expression of Th2 type cytokines was found in peripheral compared with alveolar memory T cells.CONCLUSIONS: Our data support the immunopathogenetic concept of Th1/Th2 imbalance and compartmentalisation in pulmonary sarcoidosis and suggest that the cytokine patterns change during the course of disease. Expression of Th2 type cytokines in memory lymphocytes is decreased in the alveolar compartment compared with peripheral blood.",
keywords = "Acute Disease, Adult, Bronchoalveolar Lavage Fluid/immunology, CD4-CD8 Ratio, CD4-Positive T-Lymphocytes/metabolism, CD8-Positive T-Lymphocytes/metabolism, Chronic Disease, Cytokines/blood, Female, Flow Cytometry, Humans, Male, Middle Aged, Sarcoidosis, Pulmonary/immunology, T-Lymphocyte Subsets/metabolism, Th1 Cells/metabolism, Th2 Cells/metabolism",
author = "M M{\"o}llers and Aries, {S P} and D Dr{\"o}mann and B Mascher and J Braun and K Dalhoff",
year = "2001",
month = jun,
doi = "10.1136/thorax.56.6.487",
language = "English",
volume = "56",
pages = "487--493",
journal = "THORAX",
issn = "0040-6376",
publisher = "BMJ PUBLISHING GROUP",
number = "6",

}

RIS

TY - JOUR

T1 - Intracellular cytokine repertoire in different T cell subsets from patients with sarcoidosis

AU - Möllers, M

AU - Aries, S P

AU - Drömann, D

AU - Mascher, B

AU - Braun, J

AU - Dalhoff, K

PY - 2001/6

Y1 - 2001/6

N2 - BACKGROUND: Pulmonary sarcoidosis is characterised by a mononuclear alveolitis with a predominance of CD4+ T cells and macrophages. We determined the intracellular expression of interferon (IFN)gamma, interleukin (IL)-2, tumour necrosis factor (TNF)alpha, IL-4, IL-5 and IL-10 in CD4+ and CD8+, naive and memory lymphocytes from blood and bronchoalveolar lavage (BAL) fluid using three colour flow cytometry.METHODS: Eighteen untreated patients with pulmonary sarcoidosis were evaluated and stratified according to whether they had acute or chronic disease.RESULTS: Significantly more T cells expressed Th1 than Th2 type cytokines in both BAL fluid and peripheral blood samples, regardless of clinical presentation. Significantly greater proportions of T cells secreted Th1 type cytokines in BAL fluid than in peripheral blood. Th1 type cytokines were more frequently expressed by peripheral and alveolar T cells in acute disease than in chronic disease. There were no significant differences between CD4+ and CD8+ T cells. Concerning naive and memory lymphocytes, significantly higher CD45RO:CD45RA ratios were found in BAL fluid than in blood, and increased expression of Th2 type cytokines was found in peripheral compared with alveolar memory T cells.CONCLUSIONS: Our data support the immunopathogenetic concept of Th1/Th2 imbalance and compartmentalisation in pulmonary sarcoidosis and suggest that the cytokine patterns change during the course of disease. Expression of Th2 type cytokines in memory lymphocytes is decreased in the alveolar compartment compared with peripheral blood.

AB - BACKGROUND: Pulmonary sarcoidosis is characterised by a mononuclear alveolitis with a predominance of CD4+ T cells and macrophages. We determined the intracellular expression of interferon (IFN)gamma, interleukin (IL)-2, tumour necrosis factor (TNF)alpha, IL-4, IL-5 and IL-10 in CD4+ and CD8+, naive and memory lymphocytes from blood and bronchoalveolar lavage (BAL) fluid using three colour flow cytometry.METHODS: Eighteen untreated patients with pulmonary sarcoidosis were evaluated and stratified according to whether they had acute or chronic disease.RESULTS: Significantly more T cells expressed Th1 than Th2 type cytokines in both BAL fluid and peripheral blood samples, regardless of clinical presentation. Significantly greater proportions of T cells secreted Th1 type cytokines in BAL fluid than in peripheral blood. Th1 type cytokines were more frequently expressed by peripheral and alveolar T cells in acute disease than in chronic disease. There were no significant differences between CD4+ and CD8+ T cells. Concerning naive and memory lymphocytes, significantly higher CD45RO:CD45RA ratios were found in BAL fluid than in blood, and increased expression of Th2 type cytokines was found in peripheral compared with alveolar memory T cells.CONCLUSIONS: Our data support the immunopathogenetic concept of Th1/Th2 imbalance and compartmentalisation in pulmonary sarcoidosis and suggest that the cytokine patterns change during the course of disease. Expression of Th2 type cytokines in memory lymphocytes is decreased in the alveolar compartment compared with peripheral blood.

KW - Acute Disease

KW - Adult

KW - Bronchoalveolar Lavage Fluid/immunology

KW - CD4-CD8 Ratio

KW - CD4-Positive T-Lymphocytes/metabolism

KW - CD8-Positive T-Lymphocytes/metabolism

KW - Chronic Disease

KW - Cytokines/blood

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Male

KW - Middle Aged

KW - Sarcoidosis, Pulmonary/immunology

KW - T-Lymphocyte Subsets/metabolism

KW - Th1 Cells/metabolism

KW - Th2 Cells/metabolism

U2 - 10.1136/thorax.56.6.487

DO - 10.1136/thorax.56.6.487

M3 - SCORING: Journal article

C2 - 11359967

VL - 56

SP - 487

EP - 493

JO - THORAX

JF - THORAX

SN - 0040-6376

IS - 6

ER -