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Inter-site variability of the osteocyte lacunar network in the cortical bone underpins fracture susceptibility of the superolateral femoral neck

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Inter-site variability of the osteocyte lacunar network in the cortical bone underpins fracture susceptibility of the superolateral femoral neck. / Rolvien, Tim; Vom Scheidt, Annika; Stockhausen, Kilian; Milovanovic, Petar; Djonic, Danijela; Hubert, Jan; Hawellek, Thelonius; Wacker, Alexander; Jebens, Volker; Püschel, Klaus; Zimmermann, Elizabeth A; Djuric, Marija; Amling, Michael; Busse, Björn.

In: BONE, Vol. 112, 07.2018, p. 187-193.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Rolvien, T, Vom Scheidt, A, Stockhausen, K, Milovanovic, P, Djonic, D, Hubert, J, Hawellek, T, Wacker, A, Jebens, V, Püschel, K, Zimmermann, EA, Djuric, M, Amling, M & Busse, B 2018, 'Inter-site variability of the osteocyte lacunar network in the cortical bone underpins fracture susceptibility of the superolateral femoral neck', BONE, vol. 112, pp. 187-193. https://doi.org/10.1016/j.bone.2018.04.018

APA

Rolvien, T., Vom Scheidt, A., Stockhausen, K., Milovanovic, P., Djonic, D., Hubert, J., Hawellek, T., Wacker, A., Jebens, V., Püschel, K., Zimmermann, E. A., Djuric, M., Amling, M., & Busse, B. (2018). Inter-site variability of the osteocyte lacunar network in the cortical bone underpins fracture susceptibility of the superolateral femoral neck. BONE, 112, 187-193. https://doi.org/10.1016/j.bone.2018.04.018

Vancouver

Rolvien T, Vom Scheidt A, Stockhausen K, Milovanovic P, Djonic D, Hubert J et al. Inter-site variability of the osteocyte lacunar network in the cortical bone underpins fracture susceptibility of the superolateral femoral neck. BONE. 2018 Jul;112:187-193. https://doi.org/10.1016/j.bone.2018.04.018

Bibtex

@article{237f39e531d241bbbf820bdde53007ba,
title = "Inter-site variability of the osteocyte lacunar network in the cortical bone underpins fracture susceptibility of the superolateral femoral neck",
abstract = "BACKGROUND: The osteocytic lacunar network is considered to be an integral player in the regulation of bone homeostasis, and reduction in osteocytes is associated with reduced bone strength. Here, we analyzed site-specific patterns in osteocyte characteristics and matrix composition in the cortical compartment of the femoral neck to reveal the structural basis of its fragility.METHODS: Cross-sections of the human femoral neck - one of the most common fracture sites - were acquired from 12 female cadavers (age 34-86 years) and analyzed with backscattered scanning electron microscopy and high-resolution micro-computed tomography (μ-CT). The 2D/3D density and size of the osteocyte lacunae as well as bone mineral density distribution (BMDD) were measured in two regions subject to different biomechanical loads in vivo: the inferomedial (medial) region (habitually highly loaded in compression) and the superolateral (lateral) region (lower habitual loading intensity). Using quantitative polarized light microscopy, collagen fiber orientation was quantified in these two regions, accordingly.RESULTS: In 2D measurements, the inferomedial region displayed lower mineralization heterogeneity, 19% higher osteocyte lacunar density (p = 0.005), but equal lacunar size compared to the superolateral region. 3D measurements confirmed a significantly higher osteocyte lacunar density in the inferomedial region (p = 0.015). Osteocyte lacunar density decreased in aged individuals, and inter-site differences were reduced. Site-specific osteocyte characteristics were not accompanied by changes in collagen fiber orientation.CONCLUSIONS: Since osteocyte characteristics may provide valuable insights into bone mechanical competence, the variations in osteocyte properties might reflect the increased fracture susceptibility of the superolateral neck.",
keywords = "Journal Article",
author = "Tim Rolvien and {Vom Scheidt}, Annika and Kilian Stockhausen and Petar Milovanovic and Danijela Djonic and Jan Hubert and Thelonius Hawellek and Alexander Wacker and Volker Jebens and Klaus P{\"u}schel and Zimmermann, {Elizabeth A} and Marija Djuric and Michael Amling and Bj{\"o}rn Busse",
note = "Copyright {\textcopyright} 2017. Published by Elsevier Inc.",
year = "2018",
month = jul,
doi = "10.1016/j.bone.2018.04.018",
language = "English",
volume = "112",
pages = "187--193",
journal = "BONE",
issn = "8756-3282",
publisher = "Elsevier Inc.",

}

RIS

TY - JOUR

T1 - Inter-site variability of the osteocyte lacunar network in the cortical bone underpins fracture susceptibility of the superolateral femoral neck

AU - Rolvien, Tim

AU - Vom Scheidt, Annika

AU - Stockhausen, Kilian

AU - Milovanovic, Petar

AU - Djonic, Danijela

AU - Hubert, Jan

AU - Hawellek, Thelonius

AU - Wacker, Alexander

AU - Jebens, Volker

AU - Püschel, Klaus

AU - Zimmermann, Elizabeth A

AU - Djuric, Marija

AU - Amling, Michael

AU - Busse, Björn

N1 - Copyright © 2017. Published by Elsevier Inc.

PY - 2018/7

Y1 - 2018/7

N2 - BACKGROUND: The osteocytic lacunar network is considered to be an integral player in the regulation of bone homeostasis, and reduction in osteocytes is associated with reduced bone strength. Here, we analyzed site-specific patterns in osteocyte characteristics and matrix composition in the cortical compartment of the femoral neck to reveal the structural basis of its fragility.METHODS: Cross-sections of the human femoral neck - one of the most common fracture sites - were acquired from 12 female cadavers (age 34-86 years) and analyzed with backscattered scanning electron microscopy and high-resolution micro-computed tomography (μ-CT). The 2D/3D density and size of the osteocyte lacunae as well as bone mineral density distribution (BMDD) were measured in two regions subject to different biomechanical loads in vivo: the inferomedial (medial) region (habitually highly loaded in compression) and the superolateral (lateral) region (lower habitual loading intensity). Using quantitative polarized light microscopy, collagen fiber orientation was quantified in these two regions, accordingly.RESULTS: In 2D measurements, the inferomedial region displayed lower mineralization heterogeneity, 19% higher osteocyte lacunar density (p = 0.005), but equal lacunar size compared to the superolateral region. 3D measurements confirmed a significantly higher osteocyte lacunar density in the inferomedial region (p = 0.015). Osteocyte lacunar density decreased in aged individuals, and inter-site differences were reduced. Site-specific osteocyte characteristics were not accompanied by changes in collagen fiber orientation.CONCLUSIONS: Since osteocyte characteristics may provide valuable insights into bone mechanical competence, the variations in osteocyte properties might reflect the increased fracture susceptibility of the superolateral neck.

AB - BACKGROUND: The osteocytic lacunar network is considered to be an integral player in the regulation of bone homeostasis, and reduction in osteocytes is associated with reduced bone strength. Here, we analyzed site-specific patterns in osteocyte characteristics and matrix composition in the cortical compartment of the femoral neck to reveal the structural basis of its fragility.METHODS: Cross-sections of the human femoral neck - one of the most common fracture sites - were acquired from 12 female cadavers (age 34-86 years) and analyzed with backscattered scanning electron microscopy and high-resolution micro-computed tomography (μ-CT). The 2D/3D density and size of the osteocyte lacunae as well as bone mineral density distribution (BMDD) were measured in two regions subject to different biomechanical loads in vivo: the inferomedial (medial) region (habitually highly loaded in compression) and the superolateral (lateral) region (lower habitual loading intensity). Using quantitative polarized light microscopy, collagen fiber orientation was quantified in these two regions, accordingly.RESULTS: In 2D measurements, the inferomedial region displayed lower mineralization heterogeneity, 19% higher osteocyte lacunar density (p = 0.005), but equal lacunar size compared to the superolateral region. 3D measurements confirmed a significantly higher osteocyte lacunar density in the inferomedial region (p = 0.015). Osteocyte lacunar density decreased in aged individuals, and inter-site differences were reduced. Site-specific osteocyte characteristics were not accompanied by changes in collagen fiber orientation.CONCLUSIONS: Since osteocyte characteristics may provide valuable insights into bone mechanical competence, the variations in osteocyte properties might reflect the increased fracture susceptibility of the superolateral neck.

KW - Journal Article

U2 - 10.1016/j.bone.2018.04.018

DO - 10.1016/j.bone.2018.04.018

M3 - SCORING: Journal article

C2 - 29679732

VL - 112

SP - 187

EP - 193

JO - BONE

JF - BONE

SN - 8756-3282

ER -