Internal standardization of LA-ICP-MS immuno imaging via printing of universal metal spiked inks onto tissue sections
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Internal standardization of LA-ICP-MS immuno imaging via printing of universal metal spiked inks onto tissue sections. / Hoesl, Simone; Neumann, Boris; Techritz, Sandra; Sauter, Guido; Simon, Ronald; Schlüter, Hartmut; Linscheid, Michael W; Theuring, Franz; Jakubowski, Norbert; Mueller, Larissa.
In: J ANAL ATOM SPECTROM, Vol. 31, No. 3, 03.2016, p. 801-808.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Internal standardization of LA-ICP-MS immuno imaging via printing of universal metal spiked inks onto tissue sections
AU - Hoesl, Simone
AU - Neumann, Boris
AU - Techritz, Sandra
AU - Sauter, Guido
AU - Simon, Ronald
AU - Schlüter, Hartmut
AU - Linscheid, Michael W
AU - Theuring, Franz
AU - Jakubowski, Norbert
AU - Mueller, Larissa
PY - 2016/3
Y1 - 2016/3
N2 - Formalin-fixed paraffin-embedded (FFPE) specimen from biopsy materials are a widespread sample format for pathologists and medical researchers. Pathologists are archiving vast numbers of FFPE samples which can be stored for decades. Conventional immunohistochemical staining (IHC) of biomarkers on FFPE tissue sections is one of the most important analytical techniques for cancer diagnosis and pathology in general. However standardization for IHC samples and quality management is tedious and differs significantly from clinic to clinic. Combining established IHC staining strategies with modern mass spectrometry mediated methods would increase its potential and enable access of large FFPE archives for multiplexed quantitation purposes. In this work element mass spectrometry and a new ink-jet printed internal standardization approach was successfully combined with IHC staining to facilitate quantitative multiplex assays for archived FFPE samples. The printing strategy improves elemental image resolution and reproducibility of paraffin embedded breast cancer tissue sections in laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) using conventional IHC staining as a model system to investigate the new capabilities of this technique. For the internal standardization we applied a conventional CD-ink-jet printer to print a metal spiked ink onto the top of thin layer tissue sections with constant density. Printing was carried out in a direct comparison to an iodination of the tissue section as previously described as an alternative standardization method. The use of the printed internal standard allowed correction of the fluctuation during the laser ablation process and compensated instrumental drift effects. Mediated by the ink correction approach we achieved better signal-to background-ratios (SBR) of 74 and better spatial resolution of 30 mu m compared to iodination (SBR = 23). This improved performance was demonstrated on tumorous areas in FFPE breast cancer tissue sections and allowing detection of Her-2 in tumorous areas of this tissue with significantly improved contrast
AB - Formalin-fixed paraffin-embedded (FFPE) specimen from biopsy materials are a widespread sample format for pathologists and medical researchers. Pathologists are archiving vast numbers of FFPE samples which can be stored for decades. Conventional immunohistochemical staining (IHC) of biomarkers on FFPE tissue sections is one of the most important analytical techniques for cancer diagnosis and pathology in general. However standardization for IHC samples and quality management is tedious and differs significantly from clinic to clinic. Combining established IHC staining strategies with modern mass spectrometry mediated methods would increase its potential and enable access of large FFPE archives for multiplexed quantitation purposes. In this work element mass spectrometry and a new ink-jet printed internal standardization approach was successfully combined with IHC staining to facilitate quantitative multiplex assays for archived FFPE samples. The printing strategy improves elemental image resolution and reproducibility of paraffin embedded breast cancer tissue sections in laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) using conventional IHC staining as a model system to investigate the new capabilities of this technique. For the internal standardization we applied a conventional CD-ink-jet printer to print a metal spiked ink onto the top of thin layer tissue sections with constant density. Printing was carried out in a direct comparison to an iodination of the tissue section as previously described as an alternative standardization method. The use of the printed internal standard allowed correction of the fluctuation during the laser ablation process and compensated instrumental drift effects. Mediated by the ink correction approach we achieved better signal-to background-ratios (SBR) of 74 and better spatial resolution of 30 mu m compared to iodination (SBR = 23). This improved performance was demonstrated on tumorous areas in FFPE breast cancer tissue sections and allowing detection of Her-2 in tumorous areas of this tissue with significantly improved contrast
U2 - 10.1039/c5ja00409h
DO - 10.1039/c5ja00409h
M3 - SCORING: Journal article
VL - 31
SP - 801
EP - 808
JO - J ANAL ATOM SPECTROM
JF - J ANAL ATOM SPECTROM
SN - 0267-9477
IS - 3
ER -