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Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells

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Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells. / Wolpert, Fabian; Happold, Caroline; Reifenberger, Guido; Florea, Ana-Maria; Deenen, René; Roth, Patrick; Neidert, Marian Christoph; Lamszus, Katrin; Westphal, Manfred; Weller, Michael; Eisele, Günter.

In: PLOS ONE, Vol. 10, No. 10, 2015, p. e0139603.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wolpert, F, Happold, C, Reifenberger, G, Florea, A-M, Deenen, R, Roth, P, Neidert, MC, Lamszus, K, Westphal, M, Weller, M & Eisele, G 2015, 'Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells', PLOS ONE, vol. 10, no. 10, pp. e0139603. https://doi.org/10.1371/journal.pone.0139603

APA

Wolpert, F., Happold, C., Reifenberger, G., Florea, A-M., Deenen, R., Roth, P., Neidert, M. C., Lamszus, K., Westphal, M., Weller, M., & Eisele, G. (2015). Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells. PLOS ONE, 10(10), e0139603. https://doi.org/10.1371/journal.pone.0139603

Vancouver

Wolpert F, Happold C, Reifenberger G, Florea A-M, Deenen R, Roth P et al. Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells. PLOS ONE. 2015;10(10):e0139603. https://doi.org/10.1371/journal.pone.0139603

Bibtex

@article{ca666ebfd4804a5c8abd701afee54b2d,
title = "Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells",
abstract = "Immunotherapy targeting glioblastoma initiating cells (GIC) is considered a promising strategy. However, GIC are prone to evade immune response and there is a need for potent adjuvants. IFN-β might enhance the immune response and here we define its net effect on the innate immunogenicity of GIC. The transcriptomes of GIC treated with IFN-β and controls were assessed by microarray-based expression profiling for altered expression of immune regulatory genes. Several genes involved in adaptive and innate immune responses were regulated by IFN-β. We validated these results using reverse transcription (RT)-PCR and flow cytometry for corresponding protein levels. The up-regulation of the NK cell inhibitory molecules HLA-E and MHC class I was balanced by immune stimulating effects including the up-regulation of nectin-2. In 3 out of 5 GIC lines tested we found a net immune stimulating effect of IFN-β in cytotoxicity assays using NKL cells as effectors. IFN-β therefore warrants further investigation as an adjuvant for immunotherapy targeting GIC.",
keywords = "Cell Adhesion Molecules, Cell Line, Tumor, Genes, MHC Class I, Glioblastoma, Histocompatibility Antigens Class I, Humans, Immunity, Innate, Interferon-beta, Nectins, Up-Regulation, Journal Article, Research Support, Non-U.S. Gov't",
author = "Fabian Wolpert and Caroline Happold and Guido Reifenberger and Ana-Maria Florea and Ren{\'e} Deenen and Patrick Roth and Neidert, {Marian Christoph} and Katrin Lamszus and Manfred Westphal and Michael Weller and G{\"u}nter Eisele",
year = "2015",
doi = "10.1371/journal.pone.0139603",
language = "English",
volume = "10",
pages = "e0139603",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

RIS

TY - JOUR

T1 - Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells

AU - Wolpert, Fabian

AU - Happold, Caroline

AU - Reifenberger, Guido

AU - Florea, Ana-Maria

AU - Deenen, René

AU - Roth, Patrick

AU - Neidert, Marian Christoph

AU - Lamszus, Katrin

AU - Westphal, Manfred

AU - Weller, Michael

AU - Eisele, Günter

PY - 2015

Y1 - 2015

N2 - Immunotherapy targeting glioblastoma initiating cells (GIC) is considered a promising strategy. However, GIC are prone to evade immune response and there is a need for potent adjuvants. IFN-β might enhance the immune response and here we define its net effect on the innate immunogenicity of GIC. The transcriptomes of GIC treated with IFN-β and controls were assessed by microarray-based expression profiling for altered expression of immune regulatory genes. Several genes involved in adaptive and innate immune responses were regulated by IFN-β. We validated these results using reverse transcription (RT)-PCR and flow cytometry for corresponding protein levels. The up-regulation of the NK cell inhibitory molecules HLA-E and MHC class I was balanced by immune stimulating effects including the up-regulation of nectin-2. In 3 out of 5 GIC lines tested we found a net immune stimulating effect of IFN-β in cytotoxicity assays using NKL cells as effectors. IFN-β therefore warrants further investigation as an adjuvant for immunotherapy targeting GIC.

AB - Immunotherapy targeting glioblastoma initiating cells (GIC) is considered a promising strategy. However, GIC are prone to evade immune response and there is a need for potent adjuvants. IFN-β might enhance the immune response and here we define its net effect on the innate immunogenicity of GIC. The transcriptomes of GIC treated with IFN-β and controls were assessed by microarray-based expression profiling for altered expression of immune regulatory genes. Several genes involved in adaptive and innate immune responses were regulated by IFN-β. We validated these results using reverse transcription (RT)-PCR and flow cytometry for corresponding protein levels. The up-regulation of the NK cell inhibitory molecules HLA-E and MHC class I was balanced by immune stimulating effects including the up-regulation of nectin-2. In 3 out of 5 GIC lines tested we found a net immune stimulating effect of IFN-β in cytotoxicity assays using NKL cells as effectors. IFN-β therefore warrants further investigation as an adjuvant for immunotherapy targeting GIC.

KW - Cell Adhesion Molecules

KW - Cell Line, Tumor

KW - Genes, MHC Class I

KW - Glioblastoma

KW - Histocompatibility Antigens Class I

KW - Humans

KW - Immunity, Innate

KW - Interferon-beta

KW - Nectins

KW - Up-Regulation

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1371/journal.pone.0139603

DO - 10.1371/journal.pone.0139603

M3 - SCORING: Journal article

C2 - 26441059

VL - 10

SP - e0139603

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 10

ER -