Integrated analysis of the cerebrospinal fluid peptidome and proteome.
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Integrated analysis of the cerebrospinal fluid peptidome and proteome. / Zougman, Alexandre; Pilch, Bartosz; Podtelejnikov, Alexandre; Kiehntopf, Michael; Schnabel, Claudia; Kumar, Chanchal; Mann, Matthias.
In: J PROTEOME RES, Vol. 7, No. 1, 1, 2008, p. 386-399.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Integrated analysis of the cerebrospinal fluid peptidome and proteome.
AU - Zougman, Alexandre
AU - Pilch, Bartosz
AU - Podtelejnikov, Alexandre
AU - Kiehntopf, Michael
AU - Schnabel, Claudia
AU - Kumar, Chanchal
AU - Mann, Matthias
PY - 2008
Y1 - 2008
N2 - Cerebrospinal fluid (CSF) is the only body fluid in direct contact with the brain and thus is a potential source of biomarkers. Furthermore, CSF serves as a medium of endocrine signaling and contains a multitude of regulatory peptides. A combined study of the peptidome and proteome of CSF or any other body fluid has not been reported previously. We report confident identification in CSF of 563 peptide products derived from 91 precursor proteins as well as a high confidence CSF proteome of 798 proteins. For the CSF peptidome, we use high accuracy mass spectrometry (MS) for MS and MS/MS modes, allowing unambiguous identification of neuropeptides. Combination of the peptidome and proteome data suggests that enzymatic processing of membrane proteins causes release of their extracellular parts into CSF. The CSF proteome has only partial overlap with the plasma proteome, thus it is produced locally rather than deriving from plasma. Our work offers insights into CSF composition and origin.
AB - Cerebrospinal fluid (CSF) is the only body fluid in direct contact with the brain and thus is a potential source of biomarkers. Furthermore, CSF serves as a medium of endocrine signaling and contains a multitude of regulatory peptides. A combined study of the peptidome and proteome of CSF or any other body fluid has not been reported previously. We report confident identification in CSF of 563 peptide products derived from 91 precursor proteins as well as a high confidence CSF proteome of 798 proteins. For the CSF peptidome, we use high accuracy mass spectrometry (MS) for MS and MS/MS modes, allowing unambiguous identification of neuropeptides. Combination of the peptidome and proteome data suggests that enzymatic processing of membrane proteins causes release of their extracellular parts into CSF. The CSF proteome has only partial overlap with the plasma proteome, thus it is produced locally rather than deriving from plasma. Our work offers insights into CSF composition and origin.
M3 - SCORING: Zeitschriftenaufsatz
VL - 7
SP - 386
EP - 399
JO - J PROTEOME RES
JF - J PROTEOME RES
SN - 1535-3893
IS - 1
M1 - 1
ER -
