Inherited variants affecting RNA editing may contribute to ovarian cancer susceptibility: results from a large-scale collaboration

TY - JOUR

T1 - Inherited variants affecting RNA editing may contribute to ovarian cancer susceptibility: results from a large-scale collaboration

AU - Permuth, Jennifer B

AU - Reid, Brett

AU - Earp, Madalene

AU - Chen, Ann Y

AU - Monteiro, Alvaro N A

AU - Chen, Zhihua

AU - Chenevix-Trench, Georgia

AU - Fasching, Peter A

AU - Lambrechts, Diether

AU - Vanderstichele, Adriaan

AU - Van Niewenhuyse, Els

AU - Vergote, Ignace

AU - Rossing, Mary Anne

AU - Doherty, Jennifer Anne

AU - Chang-Claude, Jenny

AU - Moysich, Kirsten B

AU - Odunsi, Kunle

AU - Goodman, Marc T

AU - Shvetsov, Yurii B

AU - Wilkens, Lynne R

AU - Thompson, Pamela J

AU - Dörk, Thilo

AU - Bogdanova, Natalia

AU - Butzow, Ralf

AU - Nevanlinna, Heli

AU - Pelttari, Liisa M

AU - Leminen, Arto

AU - Modugno, Francesmary

AU - Edwards, Robert P

AU - Ness, Roberta B

AU - Kelley, Joseph L

AU - Heitz, Florian

AU - Karlan, Beth

AU - Lester, Jenny

AU - Kjaer, Susanne Krüger

AU - Jensen, Allan

AU - Giles, Graham

AU - T Hildebrandt, Michelle A

AU - Liang, Dong

AU - Lu, Karen H

AU - Wu, Xifeng

AU - Levine, Douglas A

AU - Bisogna, Maria

AU - Berchuck, Andrew

AU - Cramer, Daniel W

AU - Terry, Kathryn L

AU - Tworoger, Shelley S

AU - Poole, Elizabeth M

AU - Bandera, Elisa V

AU - Fridley, Brooke L

AU - Cunningham, Julie M

AU - Winham, Stacey J

AU - Olson, Sara H

AU - Orlow, Irene

AU - Bjorge, Line

AU - Kiemeney, Lambertus A

AU - Massuger, Leon F A G

AU - Pejovic, Tanja

AU - Moffitt, Melissa

AU - Le, Nhu D

AU - Cook, Linda S

AU - Brooks-Wilson, Angela

AU - Kelemen, Linda E

AU - Gronwald, Jacek

AU - Lubinski, Jan

AU - Wentzensen, Nicolas

AU - Brinton, Louise A

AU - Lissowska, Jolanta

AU - Yang, Hanna P

AU - Hogdall, Estrid

AU - Hogdall, Claus

AU - Lundvall, Lene

AU - Pharoah, Paul D P

AU - Song, Honglin

AU - Campbell, Ian

AU - Eccles, Diana

AU - McNeish, Iain

AU - Whittemore, Alice S

AU - McGuire, Valerie

AU - Sieh, Weiva

AU - Rothstein, Joseph

AU - Phelan, Catherine M

AU - Risch, Harvey A

AU - Narod, Steven A

AU - McLaughlin, John R

AU - Anton-Culver, Hoda

AU - Ziogas, Argyrios

AU - Menon, Usha

AU - Gayther, Simon A

AU - Ramus, Susan J

AU - Gentry-Maharaj, Aleksandra

AU - Pearce, Celeste Leigh

AU - Wu, Anna H

AU - Kupryjanczyk, Jolanta

AU - Dansonka-Mieszkowska, Agnieszka

AU - Schildkraut, Joellen M

AU - Cheng, Jin Q

AU - Goode, Ellen L

AU - Sellers, Thomas A

AU - AOCS study group

PY - 2016/11/8

Y1 - 2016/11/8

N2 - RNA editing in mammals is a form of post-transcriptional modification in which adenosine is converted to inosine by the adenosine deaminases acting on RNA (ADAR) family of enzymes. Based on evidence of altered ADAR expression in epithelial ovarian cancers (EOC), we hypothesized that single nucleotide polymorphisms (SNPs) in ADAR genes modify EOC susceptibility, potentially by altering ovarian tissue gene expression. Using directly genotyped and imputed data from 10,891 invasive EOC cases and 21,693 controls, we evaluated the associations of 5,303 SNPs in ADAD1, ADAR, ADAR2, ADAR3, and SND1. Unconditional logistic regression was used to estimate odds ratios (OR) and 95% confidence intervals (CI), with adjustment for European ancestry. We conducted gene-level analyses using the Admixture Maximum Likelihood (AML) test and the Sequence-Kernel Association test for common and rare variants (SKAT-CR). Association analysis revealed top risk-associated SNP rs77027562 (OR (95% CI)= 1.39 (1.17-1.64), P=1.0x10-4) in ADAR3 and rs185455523 in SND1 (OR (95% CI)= 0.68 (0.56-0.83), P=2.0x10-4). When restricting to serous histology (n=6,500), the magnitude of association strengthened for rs185455523 (OR=0.60, P=1.0x10-4). Gene-level analyses revealed that variation in ADAR was associated (P<0.05) with EOC susceptibility, with PAML=0.022 and PSKAT-CR=0.020. Expression quantitative trait locus analysis in EOC tissue revealed significant associations (P<0.05) with ADAR expression for several SNPs in ADAR, including rs1127313 (G/A), a SNP in the 3' untranslated region. In summary, germline variation involving RNA editing genes may influence EOC susceptibility, warranting further investigation of inherited and acquired alterations affecting RNA editing.

AB - RNA editing in mammals is a form of post-transcriptional modification in which adenosine is converted to inosine by the adenosine deaminases acting on RNA (ADAR) family of enzymes. Based on evidence of altered ADAR expression in epithelial ovarian cancers (EOC), we hypothesized that single nucleotide polymorphisms (SNPs) in ADAR genes modify EOC susceptibility, potentially by altering ovarian tissue gene expression. Using directly genotyped and imputed data from 10,891 invasive EOC cases and 21,693 controls, we evaluated the associations of 5,303 SNPs in ADAD1, ADAR, ADAR2, ADAR3, and SND1. Unconditional logistic regression was used to estimate odds ratios (OR) and 95% confidence intervals (CI), with adjustment for European ancestry. We conducted gene-level analyses using the Admixture Maximum Likelihood (AML) test and the Sequence-Kernel Association test for common and rare variants (SKAT-CR). Association analysis revealed top risk-associated SNP rs77027562 (OR (95% CI)= 1.39 (1.17-1.64), P=1.0x10-4) in ADAR3 and rs185455523 in SND1 (OR (95% CI)= 0.68 (0.56-0.83), P=2.0x10-4). When restricting to serous histology (n=6,500), the magnitude of association strengthened for rs185455523 (OR=0.60, P=1.0x10-4). Gene-level analyses revealed that variation in ADAR was associated (P<0.05) with EOC susceptibility, with PAML=0.022 and PSKAT-CR=0.020. Expression quantitative trait locus analysis in EOC tissue revealed significant associations (P<0.05) with ADAR expression for several SNPs in ADAR, including rs1127313 (G/A), a SNP in the 3' untranslated region. In summary, germline variation involving RNA editing genes may influence EOC susceptibility, warranting further investigation of inherited and acquired alterations affecting RNA editing.

KW - Journal Article

U2 - 10.18632/oncotarget.10546

DO - 10.18632/oncotarget.10546

M3 - SCORING: Journal article

C2 - 27911851

VL - 7

SP - 72381

EP - 72394

JO - ONCOTARGET

JF - ONCOTARGET

SN - 1949-2553

IS - 45

ER -