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Induction of 8-oxo-dGTPase activity in human lymphoid cells and normal fibroblasts by oxidative stress.

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Induction of 8-oxo-dGTPase activity in human lymphoid cells and normal fibroblasts by oxidative stress. / Meyer, F; Fiala, E; Westendorf, Johannes.

In: TOXICOLOGY, Vol. 146, No. 2-3, 2-3, 2000, p. 83-92.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Meyer, F, Fiala, E & Westendorf, J 2000, 'Induction of 8-oxo-dGTPase activity in human lymphoid cells and normal fibroblasts by oxidative stress.', TOXICOLOGY, vol. 146, no. 2-3, 2-3, pp. 83-92. <http://www.ncbi.nlm.nih.gov/pubmed/10814841?dopt=Citation>

APA

Meyer, F., Fiala, E., & Westendorf, J. (2000). Induction of 8-oxo-dGTPase activity in human lymphoid cells and normal fibroblasts by oxidative stress. TOXICOLOGY, 146(2-3), 83-92. [2-3]. http://www.ncbi.nlm.nih.gov/pubmed/10814841?dopt=Citation

Vancouver

Meyer F, Fiala E, Westendorf J. Induction of 8-oxo-dGTPase activity in human lymphoid cells and normal fibroblasts by oxidative stress. TOXICOLOGY. 2000;146(2-3):83-92. 2-3.

Bibtex

@article{513bd733883e464a9cbd0a772da24c43,
title = "Induction of 8-oxo-dGTPase activity in human lymphoid cells and normal fibroblasts by oxidative stress.",
abstract = "The pre-mutagen 8-Oxo-2'-deoxyguanosine-5'-triphosphate (8-oxo-dGTP) is formed during normal cellular metabolism and its incorporation into DNA leads to transversion mutations. Human cells possess the hMTH-1 gene encoding the enzyme 8-oxo-dGTPase, which catalyzes the hydrolysis of 8-oxo-dGTP to the corresponding 8-oxo-dGMP, preventing mutations. To elucidate the involvement of 8-oxo-dGTPase in carcinogenesis, we studied hMTH-1 gene expression and enzyme activity in response to oxidative stress to human skin fibroblasts and Jurkat cells. In fibroblasts, ranges from 0 to 100 microM H(2)O(2) caused a 2-fold induction of hMTH-1-mRNA expression and a 3-fold induction of enzyme activity. A 1.7-fold induction of mRNA expression and a 3.5-fold induction of enzyme activity was obtained in Jurkat cells after treatment ranging from 0 to 300 microM H(2)O(2). Cytotoxic concentrations of hydrogen peroxide lead to an almost complete loss of enzyme activity and an inhibition of hMTH-1 mRNA expression. Induction of hMTH-1 gene expression was prevented by addition of actinomycin D and cycloheximide. These data indicate the inducibility of the hMTH-1 gene expression and enzyme activity by prooxidative molecules, such as hydrogen peroxide. These parameters can thus be used as a marker of oxidative stress.",
author = "F Meyer and E Fiala and Johannes Westendorf",
year = "2000",
language = "Deutsch",
volume = "146",
pages = "83--92",
journal = "TOXICOLOGY",
issn = "0300-483X",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

RIS

TY - JOUR

T1 - Induction of 8-oxo-dGTPase activity in human lymphoid cells and normal fibroblasts by oxidative stress.

AU - Meyer, F

AU - Fiala, E

AU - Westendorf, Johannes

PY - 2000

Y1 - 2000

N2 - The pre-mutagen 8-Oxo-2'-deoxyguanosine-5'-triphosphate (8-oxo-dGTP) is formed during normal cellular metabolism and its incorporation into DNA leads to transversion mutations. Human cells possess the hMTH-1 gene encoding the enzyme 8-oxo-dGTPase, which catalyzes the hydrolysis of 8-oxo-dGTP to the corresponding 8-oxo-dGMP, preventing mutations. To elucidate the involvement of 8-oxo-dGTPase in carcinogenesis, we studied hMTH-1 gene expression and enzyme activity in response to oxidative stress to human skin fibroblasts and Jurkat cells. In fibroblasts, ranges from 0 to 100 microM H(2)O(2) caused a 2-fold induction of hMTH-1-mRNA expression and a 3-fold induction of enzyme activity. A 1.7-fold induction of mRNA expression and a 3.5-fold induction of enzyme activity was obtained in Jurkat cells after treatment ranging from 0 to 300 microM H(2)O(2). Cytotoxic concentrations of hydrogen peroxide lead to an almost complete loss of enzyme activity and an inhibition of hMTH-1 mRNA expression. Induction of hMTH-1 gene expression was prevented by addition of actinomycin D and cycloheximide. These data indicate the inducibility of the hMTH-1 gene expression and enzyme activity by prooxidative molecules, such as hydrogen peroxide. These parameters can thus be used as a marker of oxidative stress.

AB - The pre-mutagen 8-Oxo-2'-deoxyguanosine-5'-triphosphate (8-oxo-dGTP) is formed during normal cellular metabolism and its incorporation into DNA leads to transversion mutations. Human cells possess the hMTH-1 gene encoding the enzyme 8-oxo-dGTPase, which catalyzes the hydrolysis of 8-oxo-dGTP to the corresponding 8-oxo-dGMP, preventing mutations. To elucidate the involvement of 8-oxo-dGTPase in carcinogenesis, we studied hMTH-1 gene expression and enzyme activity in response to oxidative stress to human skin fibroblasts and Jurkat cells. In fibroblasts, ranges from 0 to 100 microM H(2)O(2) caused a 2-fold induction of hMTH-1-mRNA expression and a 3-fold induction of enzyme activity. A 1.7-fold induction of mRNA expression and a 3.5-fold induction of enzyme activity was obtained in Jurkat cells after treatment ranging from 0 to 300 microM H(2)O(2). Cytotoxic concentrations of hydrogen peroxide lead to an almost complete loss of enzyme activity and an inhibition of hMTH-1 mRNA expression. Induction of hMTH-1 gene expression was prevented by addition of actinomycin D and cycloheximide. These data indicate the inducibility of the hMTH-1 gene expression and enzyme activity by prooxidative molecules, such as hydrogen peroxide. These parameters can thus be used as a marker of oxidative stress.

M3 - SCORING: Zeitschriftenaufsatz

VL - 146

SP - 83

EP - 92

JO - TOXICOLOGY

JF - TOXICOLOGY

SN - 0300-483X

IS - 2-3

M1 - 2-3

ER -